KDM1A Antibody - #DF6290

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Product: KDM1A Antibody
Catalog: DF6290
Description: Rabbit polyclonal antibody to KDM1A
Application: WB IHC
Cited expt.: WB, IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 95kDa; 93kD(Calculated).
Uniprot: O60341
RRID: AB_2838256

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MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
KDM1A Antibody detects endogenous levels of total KDM1A.
RRID:
AB_2838256
Cite Format: Affinity Biosciences Cat# DF6290, RRID:AB_2838256.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Amine oxidase (flavin containing) domain 2; Amine oxidase, flavin containing, 2; AOF2; BHC110; BRAF35 HDAC complex protein BHC110; BRAF35-HDAC complex protein BHC110; BRAF35/HDAC complex, 110-kD subunit; CPRF; EC1; FAD binding protein BRAF35 HDAC complex, 110 kDa subunit; Flavin-containing amine oxidase domain-containing protein 2; KDM 1; KDM1; Kdm1a; KDM1A_HUMAN; KIAA0601; LSD 1; LSD1; Lysine (K) specific demethylase 1; Lysine (K) specific demethylase 1A; Lysine demethylase 1A; Lysine specific histone demethylase 1; Lysine specific histone demethylase 1A; Lysine-specific demethylase 1; Lysine-specific demethylase 1A; Lysine-specific histone demethylase 1A;

Immunogens

Immunogen:

A synthesized peptide derived from human KDM1A, corresponding to a region within the internal amino acids.

Uniprot:

O60341(KDM1A_HUMAN) >>Visit HPA database.

Gene(ID):
KDM1A(23028)
Expression:
O60341 KDM1A_HUMAN:

Ubiquitously expressed.

Description:
Lysine-specific demethylase 1 (LSD1; also known as AOF2 and BHC110) is a nuclear amine oxidase homolog that acts as a histone demethylase and transcription cofactor (1). Gene activation and repression is specifically regulated by the methylation state of distinct histone protein lysine residues. For example, methylation of histone H3 at Lys4 facilitates transcriptional activation by coordinating the recruitment of BPTF, a component of the NURF chromatin remodeling complex, and WDR5, a component of multiple histone methyltransferase complexes (2,3). In contrast, methylation of histone H3 at Lys9 facilitates transcriptional repression by recruiting HP1 (4,5). LSD1 is a component of the CoREST transcriptional co-repressor complex that also contains CoREST, CtBP, HDAC1 and HDAC2. As part of this complex, LSD1 demethylates mono-methyl and di-methyl histone H3 at Lys4 through a FAD-dependent oxidation reaction to facilitate neuronal-specific gene repression in non-neuronal cells (1,6,7). In contrast, LSD1 associates with androgen receptor in human prostate cells to demethylate mono-methyl and di-methyl histone H3 at Lys9 and facilitate androgen receptor-dependent transcriptional activation (8). Therefore, depending on gene context LSD1 can function as either a transcriptional co-repressor or co-activator. LSD1 activity is inhibited by the amine oxidase inhibitors pargyline, deprenyl, clorgyline and tranylcypromine (8).
Sequence:
MLSGKKAAAAAAAAAAAATGTEAGPGTAGGSENGSEVAAQPAGLSGPAEVGPGAVGERTPRKKEPPRASPPGGLAEPPGSAGPQAGPTVVPGSATPMETGIAETPEGRRTSRRKRAKVEYREMDESLANLSEDEYYSEEERNAKAEKEKKLPPPPPQAPPEEENESEPEEPSGVEGAAFQSRLPHDRMTSQEAACFPDIISGPQQTQKVFLFIRNRTLQLWLDNPKIQLTFEATLQQLEAPYNSDTVLVHRVHSYLERHGLINFGIYKRIKPLPTKKTGKVIIIGSGVSGLAAARQLQSFGMDVTLLEARDRVGGRVATFRKGNYVADLGAMVVTGLGGNPMAVVSKQVNMELAKIKQKCPLYEANGQAVPKEKDEMVEQEFNRLLEATSYLSHQLDFNVLNNKPVSLGQALEVVIQLQEKHVKDEQIEHWKKIVKTQEELKELLNKMVNLKEKIKELHQQYKEASEVKPPRDITAEFLVKSKHRDLTALCKEYDELAETQGKLEEKLQELEANPPSDVYLSSRDRQILDWHFANLEFANATPLSTLSLKHWDQDDDFEFTGSHLTVRNGYSCVPVALAEGLDIKLNTAVRQVRYTASGCEVIAVNTRSTSQTFIYKCDAVLCTLPLGVLKQQPPAVQFVPPLPEWKTSAVQRMGFGNLNKVVLCFDRVFWDPSVNLFGHVGSTTASRGELFLFWNLYKAPILLALVAGEAAGIMENISDDVIVGRCLAILKGIFGSSAVPQPKETVVSRWRADPWARGSYSYVAAGSSGNDYDLMAQPITPGPSIPGAPQPIPRLFFAGEHTIRNYPATVHGALLSGLREAGRIADQFLGAMYTLPRQATPGVPAQQSPSM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Histone demethylase that can demethylate both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Effector of SNAI1-mediated transcription repression of E-cadherin/CDH1, CDN7 and KRT8. Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7.

PTMs:

Polyubiquitinated by JADE2; which leads to its proteasomal degradation.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed.

Family&Domains:

The SWIRM domain may act as an anchor site for a histone tail.

Belongs to the flavin monoamine oxidase family.

References

1). OTUB2 Facilitates Tumorigenesis of Gastric Cancer Through Promoting KDM1A-Mediated Stem Cell-Like Properties. Frontiers in Oncology, 2023 (PubMed: 34646768) [IF=3.5]

Application: WB    Species: Human    Sample: GC cells

Figure 5 Otubain 2 (OTUB2) increases the stability of KDM1A. (A) Relative protein levels of KDM1A in GC cells transfected with OTUB2 overexpression plasmids (MKN-45 and SGC-7901 cells) or shRNA targeting OTUB2 (HGC-27 and AGS cells). Actin served as internal control. (B) co-immunoprecipitation was conducted with anti-Flag (Flag-OTUB2) or anti-HA (HA-KDM1A) antibody and was analyzed by western blotting in 293T cells. (C) after transfected with shOTUB2, cells were treated with or without MG132. The ubiquitination level of KDM1A in MKN-45 and HGC-27 cells was measured by Western blot. Actin served as internal control. (D) After transfected with OTUB2 overexpression plasmids (MKN-45 cells) or shOTUB2, cells were treated with or without cycloheximide (CHX) for 0, 1, 2, 4, and 8 h, respectively. The protein expression levels of KDM1A were assessed by Western blot. Actin was used as internal control. Data was represented as mean ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001.

Application: IHC    Species: Human    Sample: GC cells

Figure 6 The expression of otubain 2 (OTUB2) and KDM1A in GC cells are positively correlated. (A) The expression of OTUB2, KDM1A, and CD44 was measured by immunohistochemistry in GC tissues collected from patients with GC. (B) The correlation between the expression of OTUB2, KDM1A, and CD44 in human tumor tissues was analyzed. (C) After OTUB2 was overexpressed (SGC-7901 cells) or downregulated (HGC-27 cells), the expression of KDM1A and CD44 was detected by immunohistochemistry in tumor tissues obtained from nude mice.

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