GAS2 Antibody - #DF6302

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Product: GAS2 Antibody
Catalog: DF6302
Description: Rabbit polyclonal antibody to GAS2
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 35kDa; 35kD(Calculated).
Uniprot: O43903
RRID: AB_2838268

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Bovine(92%), Horse(92%), Sheep(92%), Rabbit(100%), Dog(100%), Chicken(92%)
Clonality:
Polyclonal
Specificity:
GAS2 Antibody detects endogenous levels of total GAS2.
RRID:
AB_2838268
Cite Format: Affinity Biosciences Cat# DF6302, RRID:AB_2838268.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

GAS 2; GAS-2; Gas2; GAS2_HUMAN; Growth arrest specific 2; Growth arrest specific protein 2; Growth arrest-specific protein 2; MGC32610;

Immunogens

Immunogen:
Uniprot:

O43903(GAS2_HUMAN) >>Visit HPA database.

Gene(ID):
GAS2(2620)
Expression:
O43903 GAS2_HUMAN:

Ubiquitously expressed with highest levels in liver, lung, and kidney. Not found in spleen.

Description:
The protein encoded by this gene is a caspase-3 substrate that plays a role in regulating microfilament and cell shape changes during apoptosis. It can also modulate cell susceptibility to p53-dependent apoptosis by inhibiting calpain activity. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jan 2009]
Sequence:
MCTALSPKVRSGPGLSDMHQYSQWLASRHEANLLPMKEDLALWLTNLLGKEITAETFMEKLDNGALLCQLAETMQEKFKESMDANKPTKNLPLKKIPCKTSAPSGSFFARDNTANFLSWCRDLGVDETCLFESEGLVLHKQPREVCLCLLELGRIAARYGVEPPGLIKLEKEIEQEETLSAPSPSPSPSSKSSGKKSTGNLLDDAVKRISEDPPCKCPNKFCVERLSQGRYRVGEKILFIRMLHNKHVMVRVGGGWETFAGYLLKHDPCRMLQISRVDGKTSPIQSKSPTLKDMNPDNYLVVSASYKAKKEIK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Dog
100
Pig
92
Horse
92
Bovine
92
Sheep
92
Chicken
92
Xenopus
75
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O43903 As Substrate

Site PTM Type Enzyme
K86 Ubiquitination
T100 Phosphorylation
S101 Phosphorylation
S104 Phosphorylation
S106 Phosphorylation
S183 Phosphorylation
S185 Phosphorylation
S187 Phosphorylation
S190 Phosphorylation
T258 Phosphorylation
Y262 Phosphorylation
S282 Phosphorylation
S288 Phosphorylation

Research Backgrounds

Function:

May play a role in apoptosis by acting as a cell death substrate for caspases. Is cleaved during apoptosis and the cleaved form induces dramatic rearrangements of the actin cytoskeleton and potent changes in the shape of the affected cells. May be involved in the membrane ruffling process (By similarity).

PTMs:

Cleaved, during apoptosis, on a specific aspartic residue by caspases.

Phosphorylated on serine residues during the G0-G1 transition phase.

Subcellular Location:

Cytoplasm>Cytoskeleton>Stress fiber. Membrane>Peripheral membrane protein.
Note: Component of the microfilament system. Colocalizes with actin fibers at the cell border and along the stress fibers in growth-arrested fibroblasts. Mainly membrane-associated. When hyperphosphorylated, accumulates at membrane ruffles.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed with highest levels in liver, lung, and kidney. Not found in spleen.

Family&Domains:

Belongs to the GAS2 family.

Restrictive clause

 

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