NET1 Antibody - #DF6345
| Product: | NET1 Antibody |
| Catalog: | DF6345 |
| Description: | Rabbit polyclonal antibody to NET1 |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Zebrafish |
| Mol.Wt.: | 68kDa; 68kD(Calculated). |
| Uniprot: | Q7Z628 |
| RRID: | AB_2838309 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF6345, RRID:AB_2838309.
Fold/Unfold
ARHG8_HUMAN; ARHGEF8; GLYT2; Guanine nucleotide regulatory protein oncogene; MGC114559; MGC127767; MGC53602; mNET1; Net1; NET1A; Neuroepithelial cell transforming gene 1; Neuroepithelial cell-transforming gene 1 protein; p65 Net1 proto oncogene; Proto-oncogene p65 Net1; Rho guanine nucleotide exchange factor 8; Rho guanine nucleotide exchange factor GEF 8;
Immunogens
- Q7Z628 ARHG8_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEPELAAQKQPRPRRRSRRASGLSTEGATGPSADTSGSELDGRCSLRRGSSFTFLTPGPNWDFTLKRKRREKDDDVVSLSSLDLKEPSNKRVRPLARVTSLANLISPVRNGAVRRFGQTIQSFTLRGDHRSPASAQKFSSRSTVPTPAKRRSSALWSEMLDITMKESLTTREIRRQEAIYEMSRGEQDLIEDLKLARKAYHDPMLKLSIMSEEELTHIFGDLDSYIPLHEDLLTRIGEATKPDGTVEQIGHILVSWLPRLNAYRGYCSNQLAAKALLDQKKQDPRVQDFLQRCLESPFSRKLDLWSFLDIPRSRLVKYPLLLKEILKHTPKEHPDVQLLEDAILIIQGVLSDINLKKGESECQYYIDKLEYLDEKQRDPRIEASKVLLCHGELRSKSGHKLYIFLFQDILVLTRPVTRNERHSYQVYRQPIPVQELVLEDLQDGDVRMGGSFRGAFSNSEKAKNIFRIRFHDPSPAQSHTLQANDVFHKQQWFNCIRAAIAPFQSAGSPPELQGLPELHEECEGNHPSARKLTAQRRASTVSSVTQVEVDENAYRCGSGMQMAEDSKSLKTHQTQPGIRRARDKALSGGKRKETLV
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q7Z628 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| K9 | Ubiquitination | Uniprot | |
| S17 | Phosphorylation | Uniprot | |
| S21 | Phosphorylation | Uniprot | |
| S24 | Phosphorylation | Uniprot | |
| T25 | Phosphorylation | Uniprot | |
| T29 | Phosphorylation | Uniprot | |
| S32 | Phosphorylation | Uniprot | |
| T35 | Phosphorylation | Uniprot | |
| S36 | Phosphorylation | Uniprot | |
| S38 | Phosphorylation | Uniprot | |
| S50 | Phosphorylation | Uniprot | |
| S51 | Phosphorylation | Uniprot | |
| T53 | Phosphorylation | Uniprot | |
| T64 | Phosphorylation | Uniprot | |
| S78 | Phosphorylation | Uniprot | |
| K85 | Ubiquitination | Uniprot | |
| S100 | Phosphorylation | Q13131 (PRKAA1) | Uniprot |
| S106 | Phosphorylation | Uniprot | |
| T119 | Phosphorylation | Uniprot | |
| S122 | Phosphorylation | Uniprot | |
| R126 | Methylation | Uniprot | |
| K137 | Ubiquitination | Uniprot | |
| K149 | Ubiquitination | Uniprot | |
| S152 | Phosphorylation | Q13153 (PAK1) | Uniprot |
| Y180 | Phosphorylation | Uniprot | |
| S183 | Phosphorylation | Uniprot | |
| K194 | Ubiquitination | Uniprot | |
| K198 | Ubiquitination | Uniprot | |
| K241 | Ubiquitination | Uniprot | |
| K274 | Ubiquitination | Uniprot | |
| K280 | Ubiquitination | Uniprot | |
| K301 | Ubiquitination | Uniprot | |
| K317 | Ubiquitination | Uniprot | |
| K357 | Ubiquitination | Uniprot | |
| S360 | Phosphorylation | Uniprot | |
| K368 | Ubiquitination | Uniprot | |
| K375 | Ubiquitination | Uniprot | |
| K385 | Ubiquitination | Uniprot | |
| K396 | Ubiquitination | Uniprot | |
| S451 | Phosphorylation | Uniprot | |
| R453 | Methylation | Uniprot | |
| K489 | Ubiquitination | Uniprot | |
| S508 | Phosphorylation | Uniprot | |
| S528 | Phosphorylation | Uniprot | |
| T533 | Phosphorylation | Uniprot | |
| S539 | Phosphorylation | Uniprot | |
| T540 | Phosphorylation | Uniprot | |
| K567 | Sumoylation | Uniprot | |
| K567 | Ubiquitination | Uniprot | |
| K570 | Sumoylation | Uniprot | |
| K570 | Ubiquitination | Uniprot |
Research Backgrounds
Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the SAPK/JNK pathway Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.
Cytoplasm. Nucleus.
Widely expressed.
Interacts with RHOA in its GTP- and GDP-bound states, and with CDC42 in its GTP-bound state. Interacts with the PDZ 1 domain of BAIAP1 (By similarity).
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