STIP1 Antibody - #DF6351

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Product: STIP1 Antibody
Catalog: DF6351
Description: Rabbit polyclonal antibody to STIP1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 63kDa; 63kD(Calculated).
Uniprot: P31948
RRID: AB_2838315

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Bovine(92%), Horse(92%), Sheep(92%), Rabbit(83%), Dog(92%)
Clonality:
Polyclonal
Specificity:
STIP1 Antibody detects endogenous levels of total STIP1.
RRID:
AB_2838315
Cite Format: Affinity Biosciences Cat# DF6351, RRID:AB_2838315.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Epididymis secretory sperm binding protein Li 94n; HEL S 94n; Hop; Hsc70/Hsp90 organizing protein; Hsc70/Hsp90-organizing protein; IEF SSP 3521; NY REN 11 antigen; P60; Renal carcinoma antigen NY-REN-11; STI1; STI1L; STIP1; STIP1_HUMAN; Stress induced phosphoprotein 1; Stress-induced-phosphoprotein 1; Transformation sensitive protein IEF SSP 3521; Transformation-sensitive protein IEF SSP 3521;

Immunogens

Immunogen:
Uniprot:

P31948(STIP1_HUMAN) >>Visit HPA database.

Gene(ID):
STIP1(10963)
Description:
HOP, also known as stress-induced phospho protein 1 (STIP), is a co-chaperone to the major heat shock proteins, Hsp70 and Hsp90, and appears in early receptor complexes (1,2). Through mutual binding to both Hsp70 and Hsp90, Hop functions as an adaptor that can integrate Hsp70 and Hsp90 interactions (3,4). HOP is an abundant and highly conserved protein which is composed of three tetratricopeptide repeat (TPR) domains (TPR1, TPR2a and TPR2b) and two DP repeat domains (DP1 and DP2), whose function has not been fully resolved (5).
Sequence:
MEQVNELKEKGNKALSVGNIDDALQCYSEAIKLDPHNHVLYSNRSAAYAKKGDYQKAYEDGCKTVDLKPDWGKGYSRKAAALEFLNRFEEAKRTYEEGLKHEANNPQLKEGLQNMEARLAERKFMNPFNMPNLYQKLESDPRTRTLLSDPTYRELIEQLRNKPSDLGTKLQDPRIMTTLSVLLGVDLGSMDEEEEIATPPPPPPPKKETKPEPMEEDLPENKKQALKEKELGNDAYKKKDFDTALKHYDKAKELDPTNMTYITNQAAVYFEKGDYNKCRELCEKAIEVGRENREDYRQIAKAYARIGNSYFKEEKYKDAIHFYNKSLAEHRTPDVLKKCQQAEKILKEQERLAYINPDLALEEKNKGNECFQKGDYPQAMKHYTEAIKRNPKDAKLYSNRAACYTKLLEFQLALKDCEECIQLEPTFIKGYTRKAAALEAMKDYTKAMDVYQKALDLDSSCKEAADGYQRCMMAQYNRHDSPEDVKRRAMADPEVQQIMSDPAMRLILEQMQKDPQALSEHLKNPVIAQKIQKLMDVGLIAIR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
92
Horse
92
Bovine
92
Sheep
92
Dog
92
Rabbit
83
Zebrafish
67
Xenopus
56
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P31948 As Substrate

Site PTM Type Enzyme
M1 Acetylation
K8 Acetylation
K10 Acetylation
K13 Ubiquitination
S16 Phosphorylation
C26 S-Nitrosylation
K32 Ubiquitination
Y41 Phosphorylation
S42 Phosphorylation
R44 Methylation
Y48 Phosphorylation
Y54 Phosphorylation
Y58 Phosphorylation
C62 S-Nitrosylation
K68 Acetylation
K68 Ubiquitination
K73 Acetylation
K73 Sumoylation
K73 Ubiquitination
K78 Ubiquitination
R87 Methylation
R93 Methylation
Y95 Phosphorylation
K100 Acetylation
K100 Ubiquitination
K109 Ubiquitination
R118 Methylation
K123 Sumoylation
K123 Ubiquitination
K136 Ubiquitination
T145 Phosphorylation
S148 Phosphorylation
Y152 Phosphorylation
K162 Ubiquitination
K169 Ubiquitination
T198 Phosphorylation P06493 (CDK1)
K207 Acetylation
K210 Acetylation
K210 Sumoylation
K210 Ubiquitination
K229 Acetylation
K237 Acetylation
K246 Acetylation
K246 Ubiquitination
K252 Ubiquitination
Y261 Phosphorylation
Y269 Phosphorylation
Y275 Phosphorylation
K284 Acetylation
K284 Ubiquitination
R293 Methylation
K301 Acetylation
K301 Ubiquitination
K312 Acetylation
K312 Methylation
K317 Ubiquitination
K325 Acetylation
K325 Methylation
K325 Ubiquitination
T332 Phosphorylation P06493 (CDK1)
K337 Acetylation
K337 Ubiquitination
K344 Acetylation
K344 Ubiquitination
K347 Ubiquitination
Y354 Phosphorylation
K364 Acetylation
K364 Ubiquitination
K373 Acetylation
K373 Ubiquitination
K381 Acetylation
Y383 Phosphorylation
T384 Phosphorylation
K388 Acetylation
K388 Ubiquitination
K395 Acetylation
C417 S-Nitrosylation
C420 S-Nitrosylation
K429 Acetylation
K429 Ubiquitination
K434 Ubiquitination
K442 Ubiquitination
Y444 Phosphorylation
K446 Acetylation
K446 Ubiquitination
K453 Ubiquitination
C461 S-Nitrosylation
K462 Acetylation
K462 Ubiquitination
Y476 Phosphorylation
S481 Phosphorylation
K486 Ubiquitination
K513 Acetylation
K513 Ubiquitination
K523 Ubiquitination
K530 Acetylation
K530 Ubiquitination
K533 Ubiquitination
R543 Methylation

Research Backgrounds

Function:

Acts as a co-chaperone for HSP90AA1. Mediates the association of the molecular chaperones HSPA8/HSC70 and HSP90 (By similarity).

Subcellular Location:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Probably forms a complex composed of chaperones HSP90 and HSP70, co-chaperones STIP1/HOP, CDC37, PPP5C, PTGES3/p23, TSC1 and client protein TSC2. Forms a complex with HSPA8/HSC70, HSPCA/HSP-86 and HSPCB/HSP-84 (By similarity). Interacts with PACRG. Interacts with METTL21B. Interacts with HSP90/HSP90AA1; the interaction dissociates the PPP5C:HSP90AA1 interaction. Interacts with FLCN, FNIP1 and FNIP2. Interacts with HSPA8/HSC70 (By similarity). Interacts with HSP90AB1; upon SMYD2-dependent HSP90AB1 methylation.

Family&Domains:

The TPR 1 repeat interacts with the C-terminal of HSC70. The TPR 4, 5 and 6 repeats (also called TPR2A domain) and TPR 7, 8 and 9 repeats (also called TPR2B domain) interact with HSP90.

Research Fields

· Human Diseases > Neurodegenerative diseases > Prion diseases.

References

1). Comparative proteomics identify HSP90A, STIP1 and TAGLN‑2 in serum extracellular vesicles as potential circulating biomarkers for human adenomyosis. Experimental and Therapeutic Medicine, 2022 (PubMed: 35495589) [IF=2.4]

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