F3 Antibody | Affinity Biosciences

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Product:	F3 Antibody
Catalog:	DF6400
Description:	Rabbit polyclonal antibody to F3
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Mol.Wt.:	33kDa; 33kD(Calculated).
Uniprot:	P13726
RRID:	AB_2838363

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Secondary Antibodies
Blocking Peptides

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Clonality:

Polyclonal

Specificity:

F3 Antibody detects endogenous levels of total F3.

RRID:

AB_2838363
Cite Format: Affinity Biosciences Cat# DF6400, RRID:AB_2838363.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CD142; CD142 antigen; Coagulation factor III (thromboplastin tissue factor); Coagulation factor III; F3; FLJ17960; TF; TF_HUMAN; TFA; Thromboplastin; Tissue factor;

Immunogens

Immunogen:

Uniprot:

P13726(TF_HUMAN) >>Visit HPA database.

Gene(ID):

F3(2152)

Expression:

P13726 TF_HUMAN:

Lung, placenta and pancreas.

Description:

This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

Sequence:

P13726 TF_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

METPAWPRVPRPETAVARTLLLGWVFAQVAGASGTTNTVAAYNLTWKSTNFKTILEWEPKPVNQVYTVQISTKSGDWKSKCFYTTDTECDLTDEIVKDVKQTYLARVFSYPAGNVESTGSAGEPLYENSPEFTPYLETNLGQPTIQSFEQVGTKVNVTVEDERTLVRRNNTFLSLRDVFGKDLIYTLYYWKSSSSGKKTAKTNTNEFLIDVDKGENYCFSVQAVIPSRTVNRKSTDSPVECMGQEKGEFREIFYIIGAVVFVVIILVIILAISLHKCRKAGVGQSWKENSPLNVS

PTMs - P13726 As Substrate

P13726

Site	PTM Type	Enzyme	Source
K100	Ubiquitination		Uniprot
S120	Phosphorylation		Uniprot
S285	Phosphorylation	Q16539 (MAPK14) , P17252 (PRKCA)	Uniprot
K287	Methylation		Uniprot
K287	Ubiquitination		Uniprot
S290	Phosphorylation	P17252 (PRKCA) , Q16539 (MAPK14)	Uniprot
S295	Phosphorylation		Uniprot

Research Backgrounds

P13726_TF_HUMAN

Function:

Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.

Subcellular Location:

Membrane>Single-pass type I membrane protein.

Secreted.

Tissue Specificity:

Lung, placenta and pancreas.

Subunit Structure:

Interacts with HSPE; the interaction, inhibited by heparin, promotes the generation of activated factor X and activates coagulation in the presence of activated factor VII.

Family&Domains:

Belongs to the tissue factor family.

Research Fields

· Organismal Systems > Immune system > Complement and coagulation cascades. (View pathway)

References

1). TREM2 improves coagulopathy and lung inflammation in sepsis through the AKT-mTOR pathway. International immunopharmacology, 2025 (PubMed: 39970715) [IF=4.8]

2). Piperlongumin Improves Survival in the Mouse Model of Sepsis: Effect on Coagulation Factors and Lung Inflammation. INFLAMMATION, 2022 (PubMed: 35831643) [IF=4.5]

Application: WB Species: Mouse Sample: RAW264.7 cells

Fig. 6 PL reduces the surge in TF and PAI-1 in macrophages stimulated by LPS. A, B Each group was tested using qRT-PCR to detect TF and PAI-1 mRNA levels. C ELISA determined concentrations of PAI-1 in the supernatant of the RAW264.7 cell. D, E Western blot analysis showed protein levels of TF in RAW264.7 cells. F–I AKT, mTOR, and STAT3 phosphorylation was detected by western blot in RAW264.7 cells. Every experiment was repeated three times. Values are indicated as mean ± SD. **P

3). Pretreatment with Eupatilin Attenuates Inflammation and Coagulation in Sepsis by Suppressing JAK2/STAT3 Signaling Pathway. Journal of Inflammation Research, 2023 (PubMed: 36926276) [IF=4.5]

4). Colchicine inhibits the prothrombotic effects of oxLDL in human endothelial cells. VASCULAR PHARMACOLOGY, 2021 (PubMed: 33232770) [IF=3.5]

5). Fibroblast growth factor 2 (FGF2) ameliorates the coagulation abnormalities in sepsis. Toxicology and applied pharmacology, 2023 (PubMed: 36621722) [IF=3.3]

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F3 Antibody - #DF6400