PROC Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from ES-2, using PROC antibody.

Product:	PROC Antibody
Catalog:	DF6412
Description:	Rabbit polyclonal antibody to PROC
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Dog
Mol.Wt.:	52kDa; 52kD(Calculated).
Uniprot:	P04070
RRID:	AB_2838375

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Dog(80%)

Clonality:

Polyclonal

Specificity:

PROC Antibody detects endogenous levels of total PROC.

RRID:

AB_2838375
Cite Format: Affinity Biosciences Cat# DF6412, RRID:AB_2838375.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Activation peptide; Anticoagulant protein C; APC; Autoprothrombin IIA; Blood coagulation factor XIV; EC 3.4.21.69; PC; proC; PROC_HUMAN; PROC1; Protein C (inactivator of coagulation factors Va and VIIIa); THPH3; THPH4; Vitamin K dependent protein C;

Immunogens

Immunogen:

Uniprot:

P04070(PROC_HUMAN) >>Visit HPA database.

Gene(ID):

PROC(5624)

Expression:

P04070 PROC_HUMAN:

Plasma; synthesized in the liver.

Description:

This gene encodes a vitamin K-dependent plasma glycoprotein. The encoded protein is cleaved to its activated form by the thrombin-thrombomodulin complex. This activated form contains a serine protease domain and functions in degradation of the activated forms of coagulation factors V and VIII. Mutations in this gene have been associated with thrombophilia due to protein C deficiency, neonatal purpura fulminans, and recurrent venous thrombosis.[provided by RefSeq, Dec 2009]

Sequence:

P04070 PROC_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MWQLTSLLLFVATWGISGTPAPLDSVFSSSERAHQVLRIRKRANSFLEELRHSSLERECIEEICDFEEAKEIFQNVDDTLAFWSKHVDGDQCLVLPLEHPCASLCCGHGTCIDGIGSFSCDCRSGWEGRFCQREVSFLNCSLDNGGCTHYCLEEVGWRRCSCAPGYKLGDDLLQCHPAVKFPCGRPWKRMEKKRSHLKRDTEDQEDQVDPRLIDGKMTRRGDSPWQVVLLDSKKKLACGAVLIHPSWVLTAAHCMDESKKLLVRLGEYDLRRWEKWELDLDIKEVFVHPNYSKSTTDNDIALLHLAQPATLSQTIVPICLPDSGLAERELNQAGQETLVTGWGYHSSREKEAKRNRTFVLNFIKIPVVPHNECSEVMSNMVSENMLCAGILGDRQDACEGDSGGPMVASFHGTWFLVGLVSWGEGCGLLHNYGVYTKVSRYLDWIHGHIRDKEAPQKSWAP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Dog

Xenopus

Chicken

Rabbit

Pig

Bovine

Horse

Sheep

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P04070 As Substrate

P04070

Site	PTM Type	Source
T19	O-Glycosylation	Uniprot
K233	Acetylation	Uniprot
K234	Acetylation	Uniprot
N290	N-Glycosylation	Uniprot
S347	Phosphorylation	Uniprot
N355	N-Glycosylation	Uniprot
N371	N-Glycosylation	Uniprot

Research Backgrounds

P04070_PROC_HUMAN

Function:

Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids. Exerts a protective effect on the endothelial cell barrier function.

PTMs:

The vitamin K-dependent, enzymatic carboxylation of some Glu residues allows the modified protein to bind calcium.

N- and O-glycosylated. Partial (70%) N-glycosylation of Asn-371 with an atypical N-X-C site produces a higher molecular weight form referred to as alpha. The lower molecular weight form, not N-glycosylated at Asn-371, is beta. O-glycosylated with core 1 or possibly core 8 glycans.

The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.

May be phosphorylated on a Ser or Thr in a region (AA 25-30) of the propeptide.

Subcellular Location:

Secreted. Golgi apparatus. Endoplasmic reticulum.

Tissue Specificity:

Plasma; synthesized in the liver.

Subunit Structure:

Synthesized as a single chain precursor, which is cleaved into a light chain and a heavy chain held together by a disulfide bond. The enzyme is then activated by thrombin, which cleaves a tetradecapeptide from the amino end of the heavy chain; this reaction, which occurs at the surface of endothelial cells, is strongly promoted by thrombomodulin.

Family&Domains:

Belongs to the peptidase S1 family.

Research Fields

· Organismal Systems > Immune system > Complement and coagulation cascades. (View pathway)

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For Research Use Only.
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PROC Antibody - #DF6412