Cholinesterase/BCHE Antibody - #DF6419

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Product: Cholinesterase/BCHE Antibody
Catalog: DF6419
Description: Rabbit polyclonal antibody to Cholinesterase/BCHE
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Rabbit
Mol.Wt.: 68kDa; 68kD(Calculated).
Uniprot: P06276
RRID: AB_2838382

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(91%), Horse(91%), Rabbit(91%)
Clonality:
Polyclonal
Specificity:
Cholinesterase/BCHE Antibody detects endogenous levels of total Cholinesterase/BCHE.
RRID:
AB_2838382
Cite Format: Affinity Biosciences Cat# DF6419, RRID:AB_2838382.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Acylcholine acylhydrolase; BCHE; Butyrylcholine esterase; CHE1; CHE2; CHLE_HUMAN; Choline esterase II; Cholinesterase (serum) 2; Cholinesterase 1; Cholinesterase; E1; Pseudocholinesterase; Pseudocholinesterase E1;

Immunogens

Immunogen:
Uniprot:

P06276(CHLE_HUMAN) >>Visit HPA database.

Gene(ID):
BCHE(590)
Expression:
P06276 CHLE_HUMAN:

Detected in blood plasma (at protein level). Present in most cells except erythrocytes.

Description:
Mutant alleles at the BCHE locus are responsible for suxamethonium sensitivity. Homozygous persons sustain prolonged apnea after administration of the muscle relaxant suxamethonium in connection with surgical anesthesia. The activity of pseudocholinesterase in the serum is low and its substrate behavior is atypical. In the absence of the relaxant, the homozygote is at no known disadvantage.
Sequence:
MHSKVTIICIRFLFWFLLLCMLIGKSHTEDDIIIATKNGKVRGMNLTVFGGTVTAFLGIPYAQPPLGRLRFKKPQSLTKWSDIWNATKYANSCCQNIDQSFPGFHGSEMWNPNTDLSEDCLYLNVWIPAPKPKNATVLIWIYGGGFQTGTSSLHVYDGKFLARVERVIVVSMNYRVGALGFLALPGNPEAPGNMGLFDQQLALQWVQKNIAAFGGNPKSVTLFGESAGAASVSLHLLSPGSHSLFTRAILQSGSFNAPWAVTSLYEARNRTLNLAKLTGCSRENETEIIKCLRNKDPQEILLNEAFVVPYGTPLSVNFGPTVDGDFLTDMPDILLELGQFKKTQILVGVNKDEGTAFLVYGAPGFSKDNNSIITRKEFQEGLKIFFPGVSEFGKESILFHYTDWVDDQRPENYREALGDVVGDYNFICPALEFTKKFSEWGNNAFFYYFEHRSSKLPWPEWMGVMHGYEIEFVFGLPLERRDNYTKAEEILSRSIVKRWANFAKYGNPNETQNNSTSWPVFKSTEQKYLTLNTESTRIMTKLRAQQCRFWTSFFPKVLEMTGNIDEAEWEWKAGFHRWNNYMMDWKNQFNDYTSKKESCVGL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
91
Bovine
91
Rabbit
91
Pig
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P06276 As Substrate

Site PTM Type Enzyme
S3 Phosphorylation
T6 Phosphorylation
N45 N-Glycosylation
N85 N-Glycosylation
K88 Acetylation
N134 N-Glycosylation
Y174 Phosphorylation
T221 Phosphorylation
S226 Phosphorylation
N269 N-Glycosylation
N284 N-Glycosylation
N369 N-Glycosylation
K436 Methylation
K455 Methylation
N483 N-Glycosylation
Y505 Phosphorylation
N509 N-Glycosylation
N513 N-Glycosylation
N514 N-Glycosylation

Research Backgrounds

Function:

Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters.

PTMs:

N-glycosylated. No other PTM detected. The major N-glycan structures are of the complex diantennary type with 1 and 2 N-acetylneuraminic acid molecules (Neu5Ac) making up approximately 33% and 47% of the total N-glycans, respectively. Only low amounts of fucosylated diantennary N-glycans are detected (approximately 2%). Triantennary N-glycans with or without fucose amount to approximately 13%, whereas 5% of the total N-glycans are of the oligomannosidic or hybrid type.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in blood plasma (at protein level). Present in most cells except erythrocytes.

Subunit Structure:

Homotetramer; disulfide-linked. Dimer of dimers.

Family&Domains:

Belongs to the type-B carboxylesterase/lipase family.

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