PRDX4 Antibody | Affinity Biosciences

WB
IHC
Cites

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PRDX4 Antibody - Western blot analysis of extracts from Rat liver, using PRDX4 Antibody.

PRDX4 Antibody - DF6425 at 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P.

PRDX4 Antibody - DF6425 at 1/100 staining Mouse liver tissue by IHC-P.

PRDX4 Antibody - Fig.

Product:	PRDX4 Antibody
Catalog:	DF6425
Description:	Rabbit polyclonal antibody to PRDX4
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	31kDa; 31kD(Calculated).
Uniprot:	Q13162
RRID:	AB_2838388

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(91%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

PRDX4 Antibody detects endogenous levels of total PRDX4.

RRID:

AB_2838388
Cite Format: Affinity Biosciences Cat# DF6425, RRID:AB_2838388.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Antioxidant enzyme 372; Antioxidant enzyme AOE372; AOE37 2; AOE37-2; AOE372; EC 1.11.1.15; Peroxiredoxin IV; Peroxiredoxin-4; Peroxiredoxin4; PRDX 4; Prdx4; PRDX4_HUMAN; PRX 4; Prx IV; Prx-IV; PRX4; PrxIV; Thioredoxin dependent peroxide reductase A0372; Thioredoxin Peroxidase (Antioxidant Enzyme); Thioredoxin peroxidase; Thioredoxin peroxidase AO372; Thioredoxin-dependent peroxide reductase A0372; TRANK;

Immunogens

Immunogen:

Uniprot:

Q13162(PRDX4_HUMAN) >>Visit HPA database.

Gene(ID):

PRDX4(10549)

Description:

PRDX4(Peroxiredoxin-4) is also named as AOE37-2, Prx-IV and belongs to the AhpC/TSA family. PRDX4 is associated with acrosome formation during rat spermatogenesis and has a protective role in the male reproductive tract, because the phenotype of mice lacking this isoform includes testicular atrophy and increased sperm DNA damage(PMID:20864641). It is initially synthesized as a membrane-binding 31-kDa protein and processed into a 27-kDa secretory form and is discarded with the residual bodies(PMID:19208552). PRDX4 is a pentamer of dimers(PMID:23025503). This antibody is speific to PRDX4.

Sequence:

Q13162 PRDX4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEALPLLAATTPDHGRHRRLLLLPLLLFLLPAGAVQGWETEERPRTREEECHFYAGGQVYPGEASRVSVADHSLHLSKAKISKPAPYWEGTAVIDGEFKELKLTDYRGKYLVFFFYPLDFTFVCPTEIIAFGDRLEEFRSINTEVVACSVDSQFTHLAWINTPRRQGGLGPIRIPLLSDLTHQISKDYGVYLEDSGHTLRGLFIIDDKGILRQITLNDLPVGRSVDETLRLVQAFQYTDKHGEVCPAGWKPGSETIIPDPAGKLKYFDKLN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Xenopus

100

Zebrafish

100

Chicken

100

Rabbit

100

Dog

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13162 As Substrate

Q13162

Site	PTM Type	Source
C51	S-Nitrosylation	Uniprot
S68	Phosphorylation	Uniprot
S73	Phosphorylation	Uniprot
K78	Ubiquitination	Uniprot
K99	Acetylation	Uniprot
K99	Ubiquitination	Uniprot
K102	Ubiquitination	Uniprot
C148	S-Nitrosylation	Uniprot
R173	Methylation	Uniprot
Y188	Phosphorylation	Uniprot
Y191	Phosphorylation	Uniprot
K208	Acetylation	Uniprot
K208	Ubiquitination	Uniprot
S224	Phosphorylation	Uniprot
R230	Methylation	Uniprot
C245	S-Nitrosylation	Uniprot
K250	Methylation	Uniprot
K263	Ubiquitination	Uniprot
K265	Ubiquitination	Uniprot
Y266	Phosphorylation	Uniprot

Research Backgrounds

Q13162_PRDX4_HUMAN

Function:

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation.

PTMs:

The enzyme can be inactivated by further oxidation of the cysteine sulfenic acid (C(P)-SOH) to sulphinic acid (C(P)-SO2H) and sulphonic acid (C(P)-SO3H) instead of its condensation to a disulfide bond.

Subcellular Location:

Cytoplasm. Endoplasmic reticulum.
Note: Cotranslationally translocated to and retained within the endoplasmic reticulum. A small fraction of the protein is cytoplasmic.

Subunit Structure:

Homodimer; disulfide-linked, upon oxidation. 5 homodimers assemble to form a ring-like decamer. Can form heterodimers with PRDX1.

Family&Domains:

Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily.

References

1). Single cell analysis unveils B cell-dominated immune subtypes in HNSCC for enhanced prognostic and therapeutic stratification. International journal of oral science, 2024 (PubMed: 38622125) [IF=10.8]

Application: IHC Species: Human Sample:

Fig. 6 Unveiling a four-gene risk model for prognostication in HNSCC patients. a Comparative boxplot delineating the modeling-related gene expression levels within the two groups. b Forest plot mapping out OS-related clinical factors via single-factor Cox regression analysis. c Kaplan–Meier survival plots manifesting prognosis-linked risk scores’ impact. d ROC curve spectra, a predictive gauge for 1, 3, 5, and 10-year OS dynamics. e Comparative analysis spotlighting differential gene expressions between high and low-risk groups. f Kaplan-Meier survival curves of HNSCC patients based on four genes Risk-scores. Representative IHC staining images (g) and IHC score (h) in the two groups. Scale bar, 100 μm

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For Research Use Only.
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PRDX4 Antibody - #DF6425