TLR8 Antibody - #DF6426

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Product: TLR8 Antibody
Catalog: DF6426
Description: Rabbit polyclonal antibody to TLR8
Application: WB IHC IF/ICC
Cited expt.: IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Dog
Mol.Wt.: 115kDa; 120kD(Calculated).
Uniprot: Q9NR97
RRID: AB_2838389

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(86%), Bovine(86%), Horse(86%), Dog(86%)
Clonality:
Polyclonal
Specificity:
TLR8 Antibody detects endogenous levels of total TLR8.
RRID:
AB_2838389
Cite Format: Affinity Biosciences Cat# DF6426, RRID:AB_2838389.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CD 288; CD288; CD288 antigen; MGC119599; MGC119600; TLR 8; Tlr8; TLR8_HUMAN; Toll like receptor 8; Toll-like receptor 8;

Immunogens

Immunogen:

A synthesized peptide derived from human TLR8, corresponding to a region within C-terminal amino acids.

Uniprot:

Q9NR97(TLR8_HUMAN) >>Visit HPA database.

Gene(ID):
TLR8(51311)
Expression:
Q9NR97 TLR8_HUMAN:

Detected in brain, heart, lung, liver, placenta, in monocytes, and at lower levels in CD11c+ immature dendritic cells.

Description:
The Toll-like Receptors (TLR) are a family of human receptors that share homology with the Drosophila Toll Receptors, which are involved in mediating dorsoventral polarization in developing Drosophila embryos and participate in host immunity. The TLR family members are characterized by a highly conserved Toll homology (TH) domain, which is essential for Toll-induced signal transductions. TLRs are type I transmembrane receptors that contain an extracellular domain consisting of several leucine-rich regions and a single cytoplasmic Toll/IL-1R like domain. Three TLR family members, TLR7, TLR8 and TLR9, belong to a subfamily of TLRs which are differentially expressed. TLR7 is expressed in lung, placenta and spleen. TLR8 is expressed in lung and peripheral blood leukocytes, and TLR9 is predominantly expressed in spleen, lymph nodes, bone marrow and peripheral blood leukocytes. TLR7, TLR8 and TLR9 stimulate the NF B signaling pathway, suggesting that they play a role in the immune response.
Sequence:
MENMFLQSSMLTCIFLLISGSCELCAEENFSRSYPCDEKKQNDSVIAECSNRRLQEVPQTVGKYVTELDLSDNFITHITNESFQGLQNLTKINLNHNPNVQHQNGNPGIQSNGLNITDGAFLNLKNLRELLLEDNQLPQIPSGLPESLTELSLIQNNIYNITKEGISRLINLKNLYLAWNCYFNKVCEKTNIEDGVFETLTNLELLSLSFNSLSHVPPKLPSSLRKLFLSNTQIKYISEEDFKGLINLTLLDLSGNCPRCFNAPFPCVPCDGGASINIDRFAFQNLTQLRYLNLSSTSLRKINAAWFKNMPHLKVLDLEFNYLVGEIASGAFLTMLPRLEILDLSFNYIKGSYPQHINISRNFSKLLSLRALHLRGYVFQELREDDFQPLMQLPNLSTINLGINFIKQIDFKLFQNFSNLEIIYLSENRISPLVKDTRQSYANSSSFQRHIRKRRSTDFEFDPHSNFYHFTRPLIKPQCAAYGKALDLSLNSIFFIGPNQFENLPDIACLNLSANSNAQVLSGTEFSAIPHVKYLDLTNNRLDFDNASALTELSDLEVLDLSYNSHYFRIAGVTHHLEFIQNFTNLKVLNLSHNNIYTLTDKYNLESKSLVELVFSGNRLDILWNDDDNRYISIFKGLKNLTRLDLSLNRLKHIPNEAFLNLPASLTELHINDNMLKFFNWTLLQQFPRLELLDLRGNKLLFLTDSLSDFTSSLRTLLLSHNRISHLPSGFLSEVSSLKHLDLSSNLLKTINKSALETKTTTKLSMLELHGNPFECTCDIGDFRRWMDEHLNVKIPRLVDVICASPGDQRGKSIVSLELTTCVSDVTAVILFFFTFFITTMVMLAALAHHLFYWDVWFIYNVCLAKVKGYRSLSTSQTFYDAYISYDTKDASVTDWVINELRYHLEESRDKNVLLCLEERDWDPGLAIIDNLMQSINQSKKTVFVLTKKYAKSWNFKTAFYLALQRLMDENMDVIIFILLEPVLQHSQYLRLRQRICKSSILQWPDNPKAEGLFWQTLRNVVLTENDSRYNNMYVDSIKQY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
86
Horse
86
Bovine
86
Dog
86
Rabbit
79
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.

Subcellular Location:

Membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in brain, heart, lung, liver, placenta, in monocytes, and at lower levels in CD11c+ immature dendritic cells.

Family&Domains:

Belongs to the Toll-like receptor family.

Research Fields

· Organismal Systems > Immune system > Toll-like receptor signaling pathway.   (View pathway)

References

1). Hypoxia is correlated with the tumor immune microenvironment: Potential application of immunotherapy in bladder cancer. Cancer medicine, 2023 (PubMed: 38063246) [IF=2.9]

Application: IHC    Species: human    Sample:

FIGURE 7 Validation of the hub genes. (A) The network of hub genes of the brown module is shown. Nodes represent the genes and node size indicate the betweenness score. (B) Fluorescence graph of TLR8 knockdown in cell proliferation experiment at 100× magnification. (C) Line graph of cell proliferation and cell proliferation fold change after TLR8 knockdown. (D) Kaplan–Meier curve showed that overall survival (OS) was worse when TLR8 was highly expressed (log-rank test, p = 0.029). (E) Immunohistochemistry images of the three immunophenotypes. scale bars, 50 μm. (F) Representative images for multiplexed immunofluorescence staining (DAPI, dark blue; TLR8, red; PD-L1, purple; CD8, yellow; PD-1, orange; foxp3, white; CD56, light blue) in BLCA. Scale bar, 100 μm. (G) The cell intensity of CK+ cells, TLR8+ cells, PD-L1+ cells, CD8+ cells, PD-1+cells, foxp3+ cells and CD56+ cells. (H) Tumor tissue was recognized by CK staining and morphology. Different colors represent different distance from tumor cells. (I) The distance of mutual neighbors was represented by white lines. scale bars, 200 μm. (J) The scatter plot shows the number of total cells at different locations from the tumor cells. (K) The intensity of TLR8+ cells (left image). The scatter plot shows the number of TLR8+ cells at different locations from the tumor cells. (L) The scatter plot shows the number of PD-L1+ cells at different locations from TLR8+ cells. (M) The scatter plot shows the number of TLR8+CK+ cells, and TLR8+ PD-L1+ cells.

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