UCP3 Antibody - #DF6443

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Product: UCP3 Antibody
Catalog: DF6443
Description: Rabbit polyclonal antibody to UCP3
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Dog
Mol.Wt.: 34kDa; 34kD(Calculated).
Uniprot: P55916
RRID: AB_2838406

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(88%), Horse(88%), Dog(88%)
Clonality:
Polyclonal
Specificity:
UCP3 Antibody detects endogenous levels of total UCP3.
RRID:
AB_2838406
Cite Format: Affinity Biosciences Cat# DF6443, RRID:AB_2838406.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Mitochondrial uncoupling protein 3; SLC25A9; Solute carrier family 25 member 9; UCP 3; UCP3; UCP3_HUMAN; Uncoupling protein 3; Uncoupling protein 3 mitochondrial proton carrier;

Immunogens

Immunogen:
Uniprot:

P55916(UCP3_HUMAN) >>Visit HPA database.

Gene(ID):
UCP3(7352)
Expression:
P55916 UCP3_HUMAN:

Only in skeletal muscle and heart. Is more expressed in glycolytic than in oxidative skeletal muscles.

Description:
The uncoupling protein UCP1 (formerly designated UCP) is an integral membrane protein unique to brown adipose tissue mitochondria. UCP1 forms a dimer that acts as a proton channel, which can uncouple oxidative phosphorylation by dissipating the electrochemical potential across the inner mitochondrial membrane. This process induces heat production in brown adipose tissue and is involved in regulation of body temperature and glucose metabolism. UCP2 is a structurally related protein that also uncouples mitochondrial respiration. It is more widely expressed in human and mouse tissues, including white adipose tissue and muscle, than is UCP1. UCP2 is thought to play a role in body weight regulation. An additional UCP family member, UCP3, is highly muscle specific and is possibly involved in the uncoupling of oxidative phosphorylation in skeletal muscle.
Sequence:
MVGLKPSDVPPTMAVKFLGAGTAACFADLVTFPLDTAKVRLQIQGENQAVQTARLVQYRGVLGTILTMVRTEGPCSPYNGLVAGLQRQMSFASIRIGLYDSVKQVYTPKGADNSSLTTRILAGCTTGAMAVTCAQPTDVVKVRFQASIHLGPSRSDRKYSGTMDAYRTIAREEGVRGLWKGTLPNIMRNAIVNCAEVVTYDILKEKLLDYHLLTDNFPCHFVSAFGAGFCATVVASPVDVVKTRYMNSPPGQYFSPLDCMIKMVAQEGPTAFYKGFTPSFLRLGSWNVVMFVTYEQLKRALMKVQMLRESPF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
88
Horse
88
Dog
88
Bovine
75
Sheep
75
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P55916 As Substrate

Site PTM Type Enzyme
Y166 Phosphorylation
T168 Phosphorylation
T270 Phosphorylation
S310 Phosphorylation

Research Backgrounds

Function:

UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation. As a result, energy is dissipated in the form of heat. May play a role in the modulation of tissue respiratory control. Participates in thermogenesis and energy balance.

Subcellular Location:

Mitochondrion inner membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Only in skeletal muscle and heart. Is more expressed in glycolytic than in oxidative skeletal muscles.

Family&Domains:

Belongs to the mitochondrial carrier (TC 2.A.29) family.

References

1). Berberine attenuates the abnormal ectopic lipid deposition in skeletal muscle. Free radical biology & medicine, 2020 (PubMed: 32745766) [IF=7.1]

Application: WB    Species: mouse    Sample: skeletal muscle

Fig. 3. |Berberine promotes the expression of PGC-1α and other factors related to mitochondrial biogenesis and function in skeletal muscle.E: Representative western blots showing the key protein changes in the skeletal muscle of db/db mice treated with berberine or vehicle. All data represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 versus db/db control mice.

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