RBP4 Antibody - #BF0247

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Product: RBP4 Antibody
Catalog: BF0247
Description: Mouse monoclonal antibody to RBP4
Application: WB IHC IF/ICC ELISA FACS
Reactivity: Human
Mol.Wt.: 23kDa; 23kD(Calculated).
Uniprot: P02753
RRID: AB_2833784

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Lead Time: Same day delivery

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
ELISA 1:10000, WB 1:500-1:2000, IHC 1:200-1:1000, IF/ICC 1:200-1:1000, FCM 1:200-1:400
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Monoclonal [AFB1760]
Specificity:
RBP4 antibody detects endogenous levels of total RBP4.
RRID:
AB_2833784
Cite Format: Affinity Biosciences Cat# BF0247, RRID:AB_2833784.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

OTTHUMP00000020114; OTTHUMP00000020115; OTTHUMP00000020116; Plasma retinol binding protein 4; Plasma retinol-binding protein; Plasma retinol-binding protein(1-176); PRBP; PRO2222; RBP; RBP4; RDCCAS; RET4_HUMAN; Retinol binding protein 4; Retinol binding protein 4 interstitial; Retinol binding protein 4 plasma;

Immunogens

Immunogen:

Purified recombinant fragment of human RBP4 expressed in E. Coli.

Uniprot:

P02753(RET4_HUMAN) >>Visit HPA database.

Gene(ID):
RBP4(5950)
Expression:
P02753 RET4_HUMAN:

Detected in blood plasma and in urine (at protein level).

Description:
This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. (provided by RefSeq)
Sequence:
MKWVWALLLLAALGSGRAERDCRVSSFRVKENFDKARFSGTWYAMAKKDPEGLFLQDNIVAEFSVDETGQMSATAKGRVRLLNNWDVCADMVGTFTDTEDPAKFKMKYWGVASFLQKGNDDHWIVDTDYDTYAVQYSCRLLNLDGTCADSYSFVFSRDPNGLPPEAQKIVRQRQEELCLARQYRLIVHNGYCDGRSERNLL

PTMs - P02753 As Substrate

Site PTM Type Enzyme
Y108 Phosphorylation
Y191 Phosphorylation
S196 Phosphorylation

Research Backgrounds

Function:

Retinol-binding protein that mediates retinol transport in blood plasma. Delivers retinol from the liver stores to the peripheral tissues (Probable). Transfers the bound all-trans retinol to STRA6, that then facilitates retinol transport across the cell membrane.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in blood plasma and in urine (at protein level).

Subunit Structure:

Interacts with TTR. Interaction with TTR prevents its loss by filtration through the kidney glomeruli (Probable). Interacts with STRA6.

Family&Domains:

Belongs to the calycin superfamily. Lipocalin family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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