RBP4 Antibody - #DF6491

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Product: RBP4 Antibody
Catalog: DF6491
Description: Rabbit polyclonal antibody to RBP4
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 23kDa; 23kD(Calculated).
Uniprot: P02753
RRID: AB_2838453

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(91%), Bovine(91%), Horse(91%), Sheep(91%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
RBP4 Antibody detects endogenous levels of total RBP4.
RRID:
AB_2838453
Cite Format: Affinity Biosciences Cat# DF6491, RRID:AB_2838453.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

OTTHUMP00000020114; OTTHUMP00000020115; OTTHUMP00000020116; Plasma retinol binding protein 4; Plasma retinol-binding protein; Plasma retinol-binding protein(1-176); PRBP; PRO2222; RBP; RBP4; RDCCAS; RET4_HUMAN; Retinol binding protein 4; Retinol binding protein 4 interstitial; Retinol binding protein 4 plasma;

Immunogens

Immunogen:
Uniprot:

P02753(RET4_HUMAN) >>Visit HPA database.

Gene(ID):
RBP4(5950)
Expression:
P02753 RET4_HUMAN:

Detected in blood plasma and in urine (at protein level).

Description:
This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells.
Sequence:
MKWVWALLLLAALGSGRAERDCRVSSFRVKENFDKARFSGTWYAMAKKDPEGLFLQDNIVAEFSVDETGQMSATAKGRVRLLNNWDVCADMVGTFTDTEDPAKFKMKYWGVASFLQKGNDDHWIVDTDYDTYAVQYSCRLLNLDGTCADSYSFVFSRDPNGLPPEAQKIVRQRQEELCLARQYRLIVHNGYCDGRSERNLL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Pig
91
Horse
91
Bovine
91
Sheep
91
Zebrafish
73
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P02753 As Substrate

Site PTM Type Enzyme
Y108 Phosphorylation
Y191 Phosphorylation
S196 Phosphorylation

Research Backgrounds

Function:

Retinol-binding protein that mediates retinol transport in blood plasma. Delivers retinol from the liver stores to the peripheral tissues (Probable). Transfers the bound all-trans retinol to STRA6, that then facilitates retinol transport across the cell membrane.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in blood plasma and in urine (at protein level).

Subunit Structure:

Interacts with TTR. Interaction with TTR prevents its loss by filtration through the kidney glomeruli (Probable). Interacts with STRA6.

Family&Domains:

Belongs to the calycin superfamily. Lipocalin family.

Restrictive clause

 

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