SAA1/2 Antibody - #DF6533

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Product: SAA1/2 Antibody
Catalog: DF6533
Description: Rabbit polyclonal antibody to SAA1/2
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 14kDa; 14kD(Calculated).
Uniprot: P0DJI8 | P0DJI9
RRID: AB_2838495

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(92%), Horse(92%), Sheep(92%), Rabbit(92%), Dog(100%)
Clonality:
Polyclonal
Specificity:
SAA1/2 Antibody detects endogenous levels of total SAA1/2.
RRID:
AB_2838495
Cite Format: Affinity Biosciences Cat# DF6533, RRID:AB_2838495.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

PIG4; SAA; SAA1; SAA2; Serum amyloid A 2 protein; Serum amyloid A protein; Serum amyloid A1; Serum amyloid A2; TP53I4; Tumor protein p53 inducible protein 4;

Immunogens

Immunogen:
Uniprot:

P0DJI8(SAA1_HUMAN) >>Visit HPA database.

P0DJI9(SAA2_HUMAN) >>Visit HPA database.

Gene(ID):
SAA1(6288) | SAA2(6289)
Expression:
P0DJI8 SAA1_HUMAN:

Expressed by the liver; secreted in plasma (at protein level).

P0DJI9 SAA2_HUMAN:

Expressed by the liver; secreted in plasma.

Description:
This gene encodes a member of the serum amyloid A family of apolipoproteins. The encoded protein is a major acute phase protein that is highly expressed in response to inflammation and tissue injury. This protein also plays an important role in HDL metabolism and cholesterol homeostasis. High levels of this protein are associated with chronic inflammatory diseases including atherosclerosis, rheumatoid arthritis, Alzheimer's disease and Crohn's disease. This protein may also be a potential biomarker for certain tumors. Alternate splicing results in multiple transcript variants that encode the same protein. A pseudogene of this gene is found on chromosome 11.[provided by RefSeq, Jun 2012]
Sequence:
MKLLTGLVFCSLVLGVSSRSFFSFLGEAFDGARDMWRAYSDMREANYIGSDKYFHARGNYDAAKRGPGGVWAAEAISDARENIQRFFGHGAEDSLADQAANEWGRSGKDPNHFRPAGLPEKY

MKLLTGLVFCSLVLSVSSRSFFSFLGEAFDGARDMWRAYSDMREANYIGSDKYFHARGNYDAAKRGPGGAWAAEVISNARENIQRLTGRGAEDSLADQAANKWGRSGRDPNHFRPAGLPEKY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Horse
92
Bovine
92
Sheep
92
Rabbit
92
Pig
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P0DJI8/P0DJI9 As Substrate

Site PTM Type Enzyme
Y47 Phosphorylation
Y53 Phosphorylation
S77 Phosphorylation
S94 Phosphorylation
N101 Methylation
S106 Phosphorylation
Y122 Phosphorylation
Site PTM Type Enzyme
Y47 Phosphorylation
Y53 Phosphorylation
Y122 Phosphorylation

Research Backgrounds

Function:

Major acute phase protein.

PTMs:

This protein is the precursor of amyloid protein A, which is formed by the removal of approximately 24 residues from the C-terminal end.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed by the liver; secreted in plasma (at protein level).

Subunit Structure:

Homohexamer; dimer of trimers. Can form amyloid fibrils after partial proteolysis; the native, undenatured protein does not form amyloid fibrils (in vitro). Apolipoprotein of the HDL complex. Binds to heparin.

Family&Domains:

Belongs to the SAA family.

Function:

Major acute phase reactant. Apolipoprotein of the HDL complex.

PTMs:

This protein is the precursor of amyloid protein A, which is formed by the removal of approximately 24 residues from the C-terminal end.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed by the liver; secreted in plasma.

Family&Domains:

Belongs to the SAA family.

References

1). Fusobacterium nucleatum extracellular vesicles are enriched in colorectal cancer and facilitate bacterial adhesion. Science advances, 2024 (PubMed: 39303027) [IF=11.7]

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