Substance P Antibody | Affinity Biosciences

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Product:	Substance P Antibody
Catalog:	DF6541
Description:	Rabbit polyclonal antibody to Substance P
Application:	WB IHC IF/ICC
Cited expt.:	WB, IHC, IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Sheep, Rabbit, Dog
Mol.Wt.:	15kDa; 15kD(Calculated).
Uniprot:	P20366
RRID:	AB_2838503

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Bovine(86%), Sheep(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

Substance P Antibody detects endogenous levels of total Substance P.

RRID:

AB_2838503
Cite Format: Affinity Biosciences Cat# DF6541, RRID:AB_2838503.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

C-terminal-flanking peptide; neurokinin A; neurokinin alpha; NKA; NKNA; PPT; preprotachykinin; protachykinin; protachykinin-1; SubstanceP; TAC1; TAC2; Tachykinin 1; Tachykinin precursor 1; Tachykinin1; TKN1_HUMAN; SP;

Immunogens

Immunogen:

A synthesized peptide derived from human Substance P, corresponding to a region within N-terminal amino acids.

Uniprot:

P20366(TKN1_HUMAN) >>Visit HPA database.

Gene(ID):

TAC1(6863)

Description:

This gene encodes four products of the tachykinin peptide hormone family, substance P and neurokinin A, as well as the related peptides, neuropeptide K and neuropeptide gamma. These hormones are thought to function as neurotransmitters which interact with nerve receptors and smooth muscle cells. They are known to induce behavioral responses and function as vasodilators and secretagogues. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Sequence:

P20366 TKN1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKILVALAVFFLVSTQLFAEEIGANDDLNYWSDWYDSDQIKEELPEPFEHLLQRIARRPKPQQFFGLMGKRDADSSIEKQVALLKALYGHGQISHKRHKTDSFVGLMGKRALNSVAYERSAMQNYERRR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Sheep

100

Dog

100

Rabbit

100

Bovine

Pig

Horse

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

P20366_TKN1_HUMAN

Function:

Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.

PTMs:

The substance P form is cleaved at Pro-59 by the prolyl endopeptidase FAP (seprase) activity (in vitro).

Subcellular Location:

Secreted.

Family&Domains:

Belongs to the tachykinin family.

References

1). Ginkgolide B effectively mitigates neuropathic pain by suppressing the activation of the NLRP3 inflammasome through the induction of mitophagy in rats. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2024 (PubMed: 38908197) [IF=6.9]

2). Estrogen receptor β/substance P signaling in spinal cord mediates antinociceptive effect in a mouse model of discogenic low back pain. Frontiers in cellular neuroscience, 2023 (PubMed: 36756381) [IF=5.3]

Application: IF/ICC Species: Mouse Sample: spinal cord

Figure 3. Spatial colocalization of substance P with ERβ in the spinal cord was investigated using dual-label confocal immunofluorescence examination. The specificities of the immunopositive staining of ERβ (green) and substance P (red) were demonstrated. Substance P colocalized with ERβ in the dorsal horn of the spinal cord, mainly in the laminae I and II, a connection site of pain transmission. (A) Coronal sections through the spinal cord of DLBP mice. Scale bar: 200 μm. (B) The magnified dorsal horn areas of the spinal cord of DLBP mice. Scale bar: 20 μm.

3). Substance P Mediates Estrogen Modulation Proinflammatory Cytokines Release in Intervertebral Disc. INFLAMMATION, 2021 (PubMed: 32965648) [IF=4.5]

Application: IHC Species: mouse Sample: NP cells

Fig. 1.| Effects of ERT on SP and its receptor NK1R in NP cells. After OVX, the staining intensity of NK1R (a–c) and SP (d–f) in NP cells significantly increased compared with the sham group. While OVX+E2 could reverse these changes. Bar represents 50 μm.

4). Electroacupuncture Stimulation Alleviates Inflammatory Pain in Male Rats by Suppressing Oxidative Stress. Physiological research, 2023 (PubMed: 38015764) [IF=1.9]

Application: WB Species: Rat Sample: C6 glioma cells

Fig. 5 ROS induced proinflammatory cytokine and CIP-related substance production in C6 glioma cells. C6 cells were treated with t-BOOH (50 μM) and PBN (100 μM) for 24 h. (A): ROS were assayed by a DCFHA probe (A1: CON; C2: t-BOOH; C3: PBN); (B–D): cytokines were measured by ELISA kits; (E): expression of p-p38, p-ERK, p-p65, SP, TRPV1 and TRPV4 was determined by western blotting. GAPDH was used as a loading control. t-BOOH: tert-butyl hydroperoxide; PBN: phenyl-N-tert-butylnitrone (ROS scavenger); data are represented as the mean ± standard deviation; n=4 per group; ## P

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Substance P Antibody - #DF6541