TLR5 Antibody | Affinity Biosciences

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Figure 5 The migration of HepG2 cells induced by 25-HC is partly mediated by the up-regulation of TLR4.

Product:	TLR5 Antibody
Catalog:	DF6575
Description:	Rabbit polyclonal antibody to TLR5
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Chicken, Xenopus
Mol.Wt.:	98kDa; 98kD(Calculated).
Uniprot:	O60602
RRID:	AB_2838537

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

TLR5 Antibody detects endogenous levels of total TLR5.

RRID:

AB_2838537
Cite Format: Affinity Biosciences Cat# DF6575, RRID:AB_2838537.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

FLJ10052; MGC126430; MGC126431; SLEB1; TIL 3; TIL3; TLR 5; Tlr5; TLR5_HUMAN; Toll like receptor 5; Toll like receptor 5 precursor; Toll-like receptor 5; Toll/interleukin 1 receptor like protein 3; Toll/interleukin-1 receptor-like protein 3;

Immunogens

Immunogen:

Uniprot:

O60602(TLR5_HUMAN) >>Visit HPA database.

Gene(ID):

TLR5(7100)

Expression:

O60602 TLR5_HUMAN:

Highly expressed on the basolateral surface of intestinal epithelia (PubMed:11489966). Expressed also in other cells such as lung epithelial cells (PubMed:11489966, PubMed:18490781).

Description:

This gene encodes a member of the toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immune responses. These receptors recognize distinct pathogen-associated molecular patterns that are expressed on infectious agents. The protein encoded by this gene recognizes bacterial flagellin, the principal component of bacterial flagella and a virulence factor. The activation of this receptor mobilizes the nuclear factor NF-kappaB, which in turn activates a host of inflammatory-related target genes. Mutations in this gene have been associated with both resistance and susceptibility to systemic lupus erythematosus, and susceptibility to Legionnaire disease.[provided by RefSeq, Dec 2009]

Sequence:

O60602 TLR5_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGDHLDLLLGVVLMAGPVFGIPSCSFDGRIAFYRFCNLTQVPQVLNTTERLLLSFNYIRTVTASSFPFLEQLQLLELGSQYTPLTIDKEAFRNLPNLRILDLGSSKIYFLHPDAFQGLFHLFELRLYFCGLSDAVLKDGYFRNLKALTRLDLSKNQIRSLYLHPSFGKLNSLKSIDFSSNQIFLVCEHELEPLQGKTLSFFSLAANSLYSRVSVDWGKCMNPFRNMVLEILDVSGNGWTVDITGNFSNAISKSQAFSLILAHHIMGAGFGFHNIKDPDQNTFAGLARSSVRHLDLSHGFVFSLNSRVFETLKDLKVLNLAYNKINKIADEAFYGLDNLQVLNLSYNLLGELYSSNFYGLPKVAYIDLQKNHIAIIQDQTFKFLEKLQTLDLRDNALTTIHFIPSIPDIFLSGNKLVTLPKINLTANLIHLSENRLENLDILYFLLRVPHLQILILNQNRFSSCSGDQTPSENPSLEQLFLGENMLQLAWETELCWDVFEGLSHLQVLYLNHNYLNSLPPGVFSHLTALRGLSLNSNRLTVLSHNDLPANLEILDISRNQLLAPNPDVFVSLSVLDITHNKFICECELSTFINWLNHTNVTIAGPPADIYCVYPDSFSGVSLFSLSTEGCDEEEVLKSLKFSLFIVCTVTLTLFLMTILTVTKFRGFCFICYKTAQRLVFKDHPQGTEPDMYKYDAYLCFSSKDFTWVQNALLKHLDTQYSDQNRFNLCFEERDFVPGENRIANIQDAIWNSRKIVCLVSRHFLRDGWCLEAFSYAQGRCLSDLNSALIMVVVGSLSQYQLMKHQSIRGFVQKQQYLRWPEDFQDVGWFLHKLSQQILKKEKEKKKDNNIPLQTVATIS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Xenopus

100

Chicken

100

Pig

Horse

Bovine

Sheep

Dog

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O60602 As Substrate

O60602

Site	PTM Type	Enzyme	Source
N37	N-Glycosylation		Uniprot
N46	N-Glycosylation		Uniprot
Y108	Phosphorylation		Uniprot
Y161	Phosphorylation		Uniprot
N245	N-Glycosylation		Uniprot
N342	N-Glycosylation		Uniprot
N422	N-Glycosylation		Uniprot
N595	N-Glycosylation		Uniprot
N598	N-Glycosylation		Uniprot
K680	Acetylation		Uniprot
Y798	Phosphorylation		Uniprot
S805	Phosphorylation	Q15139 (PRKD1)	Uniprot

Research Backgrounds

O60602_TLR5_HUMAN

Function:

Pattern recognition receptor (PRR) located on the cell surface that participates in the activation of innate immunity and inflammatory response. Recognizes small molecular motifs named pathogen-associated molecular pattern (PAMPs) expressed by pathogens and microbe-associated molecular patterns (MAMPs) usually expressed by resident microbiota. Upon ligand binding such as bacterial flagellins, recruits intracellular adapter proteins MYD88 and TRIF leading to NF-kappa-B activation, cytokine secretion and induction of the inflammatory response. Plays thereby an important role in the relationship between the intestinal epithelium and enteric microbes and contributes to the gut microbiota composition throughout life (By similarity).

PTMs:

Phosphorylated at Ser-805 by PKD/PRKD1; phosphorylation induces the production of inflammatory cytokines.

Phosphorylated at Tyr-798 upon flagellin binding; required for signaling.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Tissue Specificity:

Highly expressed on the basolateral surface of intestinal epithelia. Expressed also in other cells such as lung epithelial cells.

Subunit Structure:

Homodimer. Interacts with MYD88 (via TIR domain). Interacts with TICAM1 (via TIR domain). Interacts with UNC93B1; this interaction is essential for proper TLR5 localization to the plasma membrane.

Family&Domains:

Belongs to the Toll-like receptor family.

Research Fields

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).

· Organismal Systems > Immune system > Toll-like receptor signaling pathway. (View pathway)

References

1). 25-HC promotes hepatocellular carcinoma metastasis through up-regulation of TLR4 dependent FABP4. American Journal of Cancer Research (PubMed: 31720079) [IF=5.3]

Application: WB Species: Human Sample: HepG2 cells

Figure 5 The migration of HepG2 cells induced by 25-HC is partly mediated by the up-regulation of TLR4. (A) HepG2 cells were treated with the indicated concentrations of 25-HC for 24 hours before mRNA was extracted and expressions of TLR2, TLR4, TLR5, TLR7 and TLR9 were examined by RT-qPCR. (B) HepG2 cells were treated with the indicated concentrations of 25-HC for 24 hours or treated with 25-HC at 10 μM for the indicated times before protein was collected to detect the TLR2, TLR4, TLR5, TLR7 and TLR9 expressions by Western blotting. HepG2 cells were transfected with siNC or siTLR4 or siTLR9 for 24 hours, (C) then cells were treated with the indicated concentrations of 25-HC for 24 hours before proteins were collected and the expressions of MMP1, MMP2, MMP3 and MMP9 were determined by Western blotting. (D) Or migratory ability of HepG2 cells after treated with 25-HC for 36 hours was determined by Transwell assay. Results were obtained from 3 independent experiments and are expressed as the means ± SEM. Statistical significance was determined by Student’s t-test. *P<0.05, ***P<0.001.

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TLR5 Antibody - #DF6575