TRAP220/MED1 Antibody - #DF6578

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Product: TRAP220/MED1 Antibody
Catalog: DF6578
Description: Rabbit polyclonal antibody to TRAP220/MED1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 168kDa; 168kD(Calculated).
Uniprot: Q15648
RRID: AB_2838540

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
TRAP220/MED1 Antibody detects endogenous levels of total TRAP220/MED1.
RRID:
AB_2838540
Cite Format: Affinity Biosciences Cat# DF6578, RRID:AB_2838540.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Activator-recruited cofactor 205 kDa component; ARC205; CRSP1; CRSP200; DRIP205; DRIP230; MED1; MED1_HUMAN; Mediator complex subunit 1; Mediator of RNA polymerase II transcription subunit 1; p53 regulatory protein RB18A; PBP; Peroxisome proliferator-activated receptor-binding protein; PPAR binding protein; PPAR-binding protein; PPARBP; PPARGBP; RB18A; Thyroid hormone receptor-associated protein complex 220 kDa component; Thyroid receptor-interacting protein 2; TR-interacting protein 2; Trap220; TRIP-2; TRIP2; Vitamin D receptor-interacting protein complex component DRIP205;

Immunogens

Immunogen:
Uniprot:

Q15648(MED1_HUMAN) >>Visit HPA database.

Gene(ID):
MED1(5469)
Expression:
Q15648 MED1_HUMAN:

Ubiquitously expressed.

Description:
The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. It also regulates p53-dependent apoptosis and it is essential for adipogenesis. This protein is known to have the ability to self-oligomerize. [provided by RefSeq, Jul 2008]
Sequence:
MKAQGETEESEKLSKMSSLLERLHAKFNQNRPWSETIKLVRQVMEKRVVMSSGGHQHLVSCLETLQKALKVTSLPAMTDRLESIARQNGLGSHLSASGTECYITSDMFYVEVQLDPAGQLCDVKVAHHGENPVSCPELVQQLREKNFDEFSKHLKGLVNLYNLPGDNKLKTKMYLALQSLEQDLSKMAIMYWKATNAGPLDKILHGSVGYLTPRSGGHLMNLKYYVSPSDLLDDKTASPIILHENNVSRSLGMNASVTIEGTSAVYKLPIAPLIMGSHPVDNKWTPSFSSITSANSVDLPACFFLKFPQPIPVSRAFVQKLQNCTGIPLFETQPTYAPLYELITQFELSKDPDPIPLNHNMRFYAALPGQQHCYFLNKDAPLPDGRSLQGTLVSKITFQHPGRVPLILNLIRHQVAYNTLIGSCVKRTILKEDSPGLLQFEVCPLSESRFSVSFQHPVNDSLVCVVMDVQDSTHVSCKLYKGLSDALICTDDFIAKVVQRCMSIPVTMRAIRRKAETIQADTPALSLIAETVEDMVKKNLPPASSPGYGMTTGNNPMSGTTTPTNTFPGGPITTLFNMSMSIKDRHESVGHGEDFSKVSQNPILTSLLQITGNGGSTIGSSPTPPHHTPPPVSSMAGNTKNHPMLMNLLKDNPAQDFSTLYGSSPLERQNSSSGSPRMEICSGSNKTKKKKSSRLPPEKPKHQTEDDFQRELFSMDVDSQNPIFDVNMTADTLDTPHITPAPSQCSTPPTTYPQPVPHPQPSIQRMVRLSSSDSIGPDVTDILSDIAEEASKLPSTSDDCPAIGTPLRDSSSSGHSQSTLFDSDVFQTNNNENPYTDPADLIADAAGSPSSDSPTNHFFHDGVDFNPDLLNSQSQSGFGEEYFDESSQSGDNDDFKGFASQALNTLGVPMLGGDNGETKFKGNNQADTVDFSIISVAGKALAPADLMEHHSGSQGPLLTTGDLGKEKTQKRVKEGNGTSNSTLSGPGLDSKPGKRSRTPSNDGKSKDKPPKRKKADTEGKSPSHSSSNRPFTPPTSTGGSKSPGSAGRSQTPPGVATPPIPKITIQIPKGTVMVGKPSSHSQYTSSGSVSSSGSKSHHSHSSSSSSSASTSGKMKSSKSEGSSSSKLSSSMYSSQGSSGSSQSKNSSQSGGKPGSSPITKHGLSSGSSSTKMKPQGKPSSLMNPSLSKPNISPSHSRPPGGSDKLASPMKPVPGTPPSSKAKSPISSGSGGSHMSGTSSSSGMKSSSGLGSSGSLSQKTPPSSNSCTASSSSFSSSGSSMSSSQNQHGSSKGKSPSRNKKPSLTAVIDKLKHGVVTSGPGGEDPLDGQMGVSTNSSSHPMSSKHNMSGGEFQGKREKSDKDKSKVSTSGSSVDSSKKTSESKNVGSTGVAKIIISKHDGGSPSIKAKVTLQKPGESSGEGLRPQMASSKNYGSPLISGSTPKHERGSPSHSKSPAYTPQNLDSESESGSSIAEKSYQNSPSSDDGIRPLPEYSTEKHKKHKKEKKKVKDKDRDRDRDKDRDKKKSHSIKPESWSKSPISSDQSLSMTSNTILSADRPSRLSPDFMIGEEDDDLMDVALIGN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
83
Pig
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15648 As Substrate

Site PTM Type Enzyme
K2 Methylation
S10 Phosphorylation
K15 Acetylation
K15 Ubiquitination
S18 Phosphorylation
K26 Ubiquitination
K145 Ubiquitination
S151 Phosphorylation
K152 Methylation
K168 Ubiquitination
S179 Phosphorylation
K202 Ubiquitination
T212 Phosphorylation
Y224 Phosphorylation
Y225 Phosphorylation
K235 Ubiquitination
S238 Phosphorylation
T262 Phosphorylation
K378 Ubiquitination
K426 Ubiquitination
K431 Ubiquitination
K481 Ubiquitination
K496 Ubiquitination
S503 Phosphorylation
T507 Phosphorylation
T522 Phosphorylation
K537 Ubiquitination
S588 Phosphorylation
S596 Phosphorylation
S620 Phosphorylation
T628 Phosphorylation
S634 Phosphorylation
S663 Phosphorylation
S664 Phosphorylation
S671 Phosphorylation
S672 Phosphorylation
S673 Phosphorylation
S675 Phosphorylation
S682 Phosphorylation
S684 Phosphorylation
T687 Phosphorylation
T704 Phosphorylation O14757 (CHEK1)
S719 Phosphorylation
S770 Phosphorylation
S771 Phosphorylation
S772 Phosphorylation
S774 Phosphorylation
T780 Phosphorylation
S784 Phosphorylation
S795 Phosphorylation
T805 Phosphorylation
S816 Phosphorylation Q13315 (ATM)
S848 Phosphorylation
S850 Phosphorylation
S851 Phosphorylation
S853 Phosphorylation
S872 Phosphorylation Q13315 (ATM)
S874 Phosphorylation Q13315 (ATM)
S887 Phosphorylation Q13315 (ATM)
S900 Phosphorylation
T928 Phosphorylation
S951 Phosphorylation
S953 Phosphorylation Q13315 (ATM)
K965 Acetylation
K973 Acetylation
K991 Acetylation
K994 Acetylation
S1000 Phosphorylation
K1004 Acetylation
K1006 Methylation
K1011 Acetylation
S1021 Phosphorylation
S1023 Phosphorylation
T1032 Phosphorylation P27361 (MAPK3) , P28482 (MAPK1)
S1036 Phosphorylation
S1040 Phosphorylation
S1042 Phosphorylation
S1045 Phosphorylation
S1049 Phosphorylation
T1051 Phosphorylation
T1057 Phosphorylation
K1076 Acetylation
S1081 Phosphorylation
S1088 Phosphorylation
S1092 Phosphorylation
K1126 Acetylation
S1128 Phosphorylation
Y1132 Phosphorylation
S1133 Phosphorylation
S1134 Phosphorylation Q13315 (ATM)
S1141 Phosphorylation Q13315 (ATM)
S1147 Phosphorylation Q13315 (ATM)
S1149 Phosphorylation
S1155 Phosphorylation
S1156 Phosphorylation
T1159 Phosphorylation
K1160 Methylation
K1173 Acetylation
K1177 Acetylation
S1187 Phosphorylation
K1188 Acetylation
S1192 Phosphorylation
S1207 Phosphorylation P24941 (CDK2)
K1210 Acetylation
T1215 Phosphorylation P24941 (CDK2)
S1218 Phosphorylation
S1223 Phosphorylation
S1226 Phosphorylation
S1227 Phosphorylation
S1229 Phosphorylation
S1232 Phosphorylation
S1239 Phosphorylation
S1241 Phosphorylation
S1251 Phosphorylation
S1252 Phosphorylation
S1254 Phosphorylation
S1256 Phosphorylation
T1259 Phosphorylation
S1302 Phosphorylation
K1309 Acetylation
S1347 Phosphorylation
K1354 Acetylation
S1368 Phosphorylation
K1376 Acetylation
T1378 Phosphorylation
T1387 Phosphorylation
S1401 Phosphorylation
S1403 Phosphorylation
Y1431 Phosphorylation
S1433 Phosphorylation
S1437 Phosphorylation
S1439 Phosphorylation
T1440 Phosphorylation
S1449 Phosphorylation
S1451 Phosphorylation
S1453 Phosphorylation
Y1456 Phosphorylation
T1457 Phosphorylation P27361 (MAPK3) , P28482 (MAPK1) , P78527 (PRKDC)
S1463 Phosphorylation
S1465 Phosphorylation
S1467 Phosphorylation
S1475 Phosphorylation
Y1476 Phosphorylation
S1479 Phosphorylation
S1481 Phosphorylation
S1482 Phosphorylation
K1502 Acetylation
K1504 Acetylation
K1529 Acetylation
S1532 Phosphorylation
S1534 Phosphorylation
S1536 Phosphorylation
S1548 Phosphorylation
T1550 Phosphorylation

Research Backgrounds

Function:

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Acts as a coactivator for GATA1-mediated transcriptional activation during erythroid differentiation of K562 erythroleukemia cells.

PTMs:

Phosphorylated by MAPK1 or MAPK3 during G2/M phase which may enhance protein stability and promote entry into the nucleolus. Phosphorylation increases its interaction with PSIP1.

Subcellular Location:

Nucleus.
Note: A subset of the protein may enter the nucleolus subsequent to phosphorylation by MAPK1 or MAPK3.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed.

Subunit Structure:

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. This subunit specifically interacts with a number of nuclear receptors in a ligand-dependent fashion including AR, ESR1, ESR2, PPARA, PPARG, RORA, RXRA, RXRG, THRA, THRB and VDR. Interacts with CTNNB1, GABPA, GLI3, PPARGC1A and TP53. Interacts with YWHAH. Interacts with CLOCK; this interaction requires the presence of THRAP3 (By similarity). Interacts with GATA1 and CCAR1. Interacts with NR4A3 (By similarity). Interacts (via IBM motif) with PSIP1 (via IBD domain); phosphorylation increases its affinity for PSIP1.

Family&Domains:

Belongs to the Mediator complex subunit 1 family.

Research Fields

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

References

1). HOXA5 induces M2 macrophage polarization to attenuate carotid atherosclerosis by activating MED1. IUBMB LIFE, 2021 (PubMed: 34117711) [IF=3.7]

Application: WB    Species: Mouse    Sample: RAW264.7 cells

FIGURE 6 HOXA5 promoted M2 macrophage polarization by the co-regulation of MED1 in carotid arteries. (a) Immunofluorescent images for the colocalization of HOXA5 and CD163. (b, d) Relative mRNA levels of M1 (MARCO and MHCII) and M2 (PPARγ, Arg1, and MRC1) markers were detected by QPCR. (c, e) The levels of IL-6 and IL-10 were determined by ELISA. (f, g) Relative mRNA and protein levels of MED1 were examined using QPCR and Western blot. (h) Immunofluorescent images for the co-localization of MED1 and MAC- 3. & p < .05. n = 6. ELISA, enzyme-linked immunosorbent assay; HOXA5, homeobox A5; MED1, mediator subunit 1; QPCR, quantitative real-time reverse transcription PCR

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