PTBP1 Antibody - #DF6644

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Product: PTBP1 Antibody
Catalog: DF6644
Description: Rabbit polyclonal antibody to PTBP1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep
Mol.Wt.: 57kDa; 57kD(Calculated).
Uniprot: P26599
RRID: AB_2838606

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%)
Clonality:
Polyclonal
Specificity:
PTBP1 Antibody detects endogenous levels of total PTBP1.
RRID:
AB_2838606
Cite Format: Affinity Biosciences Cat# DF6644, RRID:AB_2838606.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

57 kDa RNA binding protein PPTB 1; 57 kDa RNA-binding protein PPTB-1; Heterogeneous nuclear ribonucleoprotein I; Heterogeneous Nuclear Ribonucleoprotein Polypeptide I; hnRNP I; HNRNP-I; HNRNPI; HNRPI; MGC10830; MGC8461; Polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoprotein I); Polypyrimidine Tract Binding Protein 1; Polypyrimidine tract binding protein; Polypyrimidine tract-binding protein 1; pPTB; PTB 1; PTB 2; PTB 3; PTB 4; PTB; PTB T; PTB1; PTB2; PTB3; PTB4; PTBP 1; PTBP1; PTBP1_HUMAN; PTBT; RNA Binding Protein;

Immunogens

Immunogen:
Uniprot:

P26599(PTBP1_HUMAN) >>Visit HPA database.

Gene(ID):
PTBP1(5725)
Description:
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. This protein is localized in the nucleoplasm and it is also detected in the perinucleolar structure. Alternatively spliced transcript variants encoding different isoforms have been described.
Sequence:
MDGIVPDIAVGTKRGSDELFSTCVTNGPFIMSSNSASAANGNDSKKFKGDSRSAGVPSRVIHIRKLPIDVTEGEVISLGLPFGKVTNLLMLKGKNQAFIEMNTEEAANTMVNYYTSVTPVLRGQPIYIQFSNHKELKTDSSPNQARAQAALQAVNSVQSGNLALAASAAAVDAGMAMAGQSPVLRIIVENLFYPVTLDVLHQIFSKFGTVLKIITFTKNNQFQALLQYADPVSAQHAKLSLDGQNIYNACCTLRIDFSKLTSLNVKYNNDKSRDYTRPDLPSGDSQPSLDQTMAAAFGLSVPNVHGALAPLAIPSAAAAAAAAGRIAIPGLAGAGNSVLLVSNLNPERVTPQSLFILFGVYGDVQRVKILFNKKENALVQMADGNQAQLAMSHLNGHKLHGKPIRITLSKHQNVQLPREGQEDQGLTKDYGNSPLHRFKKPGSKNFQNIFPPSATLHLSNIPPSVSEEDLKVLFSSNGGVVKGFKFFQKDRKMALIQMGSVEEAVQALIDLHNHDLGENHHLRVSFSKSTI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P26599 As Substrate

Site PTM Type Enzyme
M1 Acetylation
T12 Phosphorylation
K13 Sumoylation
K13 Ubiquitination
S16 Phosphorylation P17612 (PRKACA)
S21 Phosphorylation
T22 Phosphorylation
T25 O-Glycosylation
S32 Phosphorylation
S35 Phosphorylation
S37 Phosphorylation
K45 Acetylation
K46 Acetylation
S51 Phosphorylation
R52 Methylation
S53 Phosphorylation
K65 Sumoylation
K65 Ubiquitination
K84 Sumoylation
K84 Ubiquitination
K92 Ubiquitination
K94 Ubiquitination
Y127 Phosphorylation
S131 Phosphorylation
K134 Acetylation
K134 Ubiquitination
K137 Sumoylation
K137 Ubiquitination
T138 Phosphorylation
S140 Phosphorylation
S141 Phosphorylation
S159 Phosphorylation
S167 Phosphorylation
S181 Phosphorylation
Y193 Phosphorylation
K212 Ubiquitination
K218 Sumoylation
K218 Ubiquitination
Y228 Phosphorylation
S233 Phosphorylation
C250 S-Nitrosylation
C251 S-Nitrosylation
K259 Acetylation
K259 Sumoylation
K259 Ubiquitination
K266 Acetylation
K266 Sumoylation
K266 Ubiquitination
Y267 Phosphorylation
K271 Ubiquitination
S272 Phosphorylation
S337 Phosphorylation
S342 Phosphorylation
K368 Acetylation
K368 Ubiquitination
K374 Ubiquitination
S392 Phosphorylation
K402 Ubiquitination
K410 Acetylation
K410 Ubiquitination
T427 Phosphorylation
K428 Ubiquitination
Y430 Phosphorylation
S433 Phosphorylation
S443 Phosphorylation
S466 Phosphorylation
S476 Phosphorylation
K482 Ubiquitination
K485 Acetylation
K485 Sumoylation
K485 Ubiquitination
K489 Acetylation
K489 Ubiquitination
K528 Acetylation
K528 Ubiquitination
S529 Phosphorylation
T530 Phosphorylation

Research Backgrounds

Function:

Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Activates exon skipping of its own pre-mRNA during muscle cell differentiation. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA. Represses the splicing of MAPT/Tau exon 10. In case of infection by picornaviruses, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Monomer. Part of a ternary complex containing KHSRP, PTBP1, PTBP2 and HNRPH1. Interacts with RAVER1 and SFPQ.

References

1). PTBP1 knockdown promotes neural differentiation of glioblastoma cells through UNC5B receptor. Theranostics, 2022 (PubMed: 35664063) [IF=12.4]

Application: WB    Species: Human    Sample: sh-Luci and sh-PTBP1-3 U251 cells

Figure 1 PTBP1 knockdown reprograms glioblastoma cells into a neural differentiation state. (A) Knockdown efficiency of three different sh-PTBP1 lentiviruses detected by qRT-PCR (n = 3). (B) Western blot analysis of PTBP1 protein in sh-Luci and sh-PTBP1-3 U251 cells (n = 3). GAPDH was used as an internal reference protein. (C) The scheme of cell reprogramming. More than 90% of U251 (D, G), U87 (E, H), KNS89 (F, I) cells expressed neuron markers (TUJ1 and MAP2) at 14 dpi (9 random fields from triplicate samples were captured for quantification; 829 U251, 204 U87 and 540 KNS89 cells (M-cherry+) were tracked per field in sh-Luci group; 164 U251, 20 U87 and 166 KNS89 cells (M-cherry+) were tracked per field in sh-PTBP1 group). The data are presented as mean ± SD. **P < 0.01, ***P < 0.001 vs. sh-Luci group. GM: glioblastoma cell medium; ND: not detected; NM: neuronal induction medium. Scale: 100 µm.

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