SP-C Antibody - #DF6647

Fig. 4: Activation of TRβ constrains the metaplasia and hyperplasia of AT2 cells in fibrosis. a The uniform manifold approximation and projection (UMAP) plot displays cells colored by cell type identity in scRNA-seq. b UMAP visualization of Thrb regulon activity. c GO enrichment analysis of GC-1-regulated genes in differential expression between BLM and PBS groups in mouse lung bulk RNA-seq (n = 3). d Immunochemistry staining for SFTPC and KRT8 in lung sections of mice, same as Fig. 3. Arrows indicate normal AT2 cells, and arrowheads indicate elongated or hypertrophic AT2 cells. Scale bar, 100 μm. e Protein analysis in lung homogenates for M-AT2 cell marker CLDN4. f, g Workflow, representative IF images, and quantification of Ki67-positive AT2 cells in lineage-labeled mice lungs with or without GC-1 after BLM challenge. Arrowheads indicate basaloid cells from lineage-labeled cells (indicated by arrows) (n = 3). Scale bar, 50 μm. h Representative pictures and quantification of EdU assay (n = 12, images from four experiments) in A549. GC-1 15 nM for 36 h. Scale bar, 100 μm. i–l AT2 cells isolated from different groups were used to stimulate normal primary fibroblasts for 48 h. The activation of lung fibroblasts was assayed by the 3D-collagen gel contraction (j, n = 3), expression of a-SMA (k, Scale bar, 20 μm), COL1A1, and FN1 (l). The value of n indicates biologically independent samples (c, g, j). Data of different views from four biologically independent samples (h). Similar results were repeated in two biologically independent experiments. Significant differences were assessed using two-tailed unpaired Student’s t test (h) and one-way ANOVA with Tukey test (g, j). Results are presented as mean ± SEM.

Fig. 2. Analysis of lung development; itaconic acid (ITA) improves the alveolar simplification in BPD rats. A Flow chart of hyperoxia-induced BPD rat model. Control group pups are housed in room air (21%), BPD group pups are housed in O2-enriched air (85%) and injected daily with solvent, and age-matched O2-exposed (85%) pups were injected daily with itaconic acid (ITA; 100 mg/kg) until 14 days of age (Created with BioRender.com). B The body weights and mortality in BPD VS control group. C Newborn rats are sacrificed on the 7th and 14th day post birth. Lung morphometry, including the quantification of terminal air spaces, secondary septa counts, and mean linear intercept, is analyzed using hematoxylin and eosin staining of representative lung tissue sections. N = 3 animals/group. Bar = 100 μm. Values are expressed as mean ± SD. **p < 0.01, ***p < 0.001, ****p < 0.0001. D Immunofluorescence staining of SP-C (red) and AQP-5 (green) in lung tissues at P7. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Fig. 1 Single-walled carbon nanotubes (SWCNTs) induced pulmonary fibrosis and senescence of AECIIs in mouse lung tissues. Mice were exposed to 40 μg SWCNT by intratracheal instillation, then lung tissues were collected on days 3, 7 and 28. A Scheme of workflow for evaluation of cellular senescence and pulmonary fibrosis induced by SWCNTs in vivo. B–D Western Blotting analysis of p21 and p16 protein expressions (n = 3) in lung tissues. Contents of TGF-β (E) and PAI-1 (F) in bronchoalveolar lavage fluid (BALF) quantified by ELISA (n = 5). HE staining (G) and Masson’s trichrome staining (H) of mouse lung tissues (200×). I The semiquantitative Ashcroft scores for the severity of pulmonary fibrosis (n = 4). J The hydroxyproline (HYP) level (n = 4) in lung tissues of mice. K Immunohistochemistry (IHC) of COL I expression (n = 5) in lung tissues of mice. L, M The correlation of hydroxyproline contents in mice lung tissues and senescence-associated secretory phenotype (SASP) factors (TGF-β and PAI-1) in BALF. N Immunostaining for p16 (a senescence-related marker) and SP-C (an AECIIs marker) from SWCNTs-exposed lung tissues of mice on day 28 was detected by immunofluorescence (IF) (400×). *P 

Fig. 5 UXM analysis identified various cell populations in EM-seq and were verified by Immunohistochemistry (A) Cell proportion score between tumor (red) and normal (green) tissue analyzed by UXM. (B) The cellular composition of each tumor and normal tissue identified by UXM. (C) Representative immunohistochemistry image shows that CD3、CD19、SP-C were highly expressed in NSCLC tumor tissues (C-E); CD31were highly expressed in normal lung tissue (F); CD56 were weakly expressed in normal lung tissue (G).

Fig. 7: The effect of lipid micelle-encapsulated GW806742X targeting MLKL in AT II in ALI. a The process of preparing SPC-modified PEG-DSPE micelle containing GW806742X(SPC-M-GW) targeting AT II. b Transmission electron micrographs of lipid nanomicelles with unconjugated antibody (M-GW) and conjugated antibody (SPC-M-GW), the scale length is 100 nm. c, d Statistical plots of the hydration diameters and zeta potentials of the M-GW and the SPC-M-GW. e Fluorescence microscope images of mouse lung tissue stained with DAPI, SPC and Cy5 can be seen to appear in aggregates in AT II. f Confocal microscopy images of MLE, with or without treatment, where blue (DAPI) represents the cell nucleus, red (Tubulin) represents the cytoskeleton, and green illustrates the distribution of MLKL within the cells, the scale bar is 10 µm. g-i The changes of mRNA levels for IL-1β, IL-6, TNF-α in the lung tissues in Control, ALI and ALI/SPC-M-GW 1 mg/kg treatment groups. j The total cell count in BALF. k Immunohistochemical staining images of E-cadherin protein in mouse lung tissue. l Microscope images of HE-stained lung tissues of mice with LPS, GW (GW806742X) or SPC-M-GW, the dose for two groups of GW is 1 mg/kg, and lung injury was scored (m). n Survival curve of ALI and SPC-M-GW treatment mice. After airway instillation for modeling and treatment, the number of deaths and body weight of mice were recorded daily. Mice were considered deceased when their body weight dropped more than 20% from the original weight. Significance was determined with p-values