RPGR Antibody | Affinity Biosciences

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RPGR Antibody - Western blot analysis of Mouse heart lysates, using RPGR Antibody.

Product:	RPGR Antibody
Catalog:	DF6654
Description:	Rabbit polyclonal antibody to RPGR
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Dog
Mol.Wt.:	113kDa; 113kD(Calculated).
Uniprot:	Q92834
RRID:	AB_2838616

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(80%), Dog(80%)

Clonality:

Polyclonal

Specificity:

RPGR Antibody detects endogenous levels of total RPGR.

RRID:

AB_2838616
Cite Format: Affinity Biosciences Cat# DF6654, RRID:AB_2838616.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

COD1; Cone Dystrophy 1 (X-Linked); CORDX1; CRD; Orf15; PCDX; Retinitis Pigmentosa 15; Retinitis Pigmentosa GTPase Regulator; RP15; RP3; RPGR; RPGR_HUMAN; X-linked retinitis pigmentosa GTPase regulator; XLRP3;

Immunogens

Immunogen:

Uniprot:

Q92834(RPGR_HUMAN) >>Visit HPA database.

Gene(ID):

RPGR(6103)

Expression:

Q92834 RPGR_HUMAN:

Heart, brain, placenta, lung, liver, muscle, kidney, retina, pancreas and fetal retinal pigment epithelium. Isoform 3 is found only in the retina. Colocalizes with RPGRIP1 in the outer segment of rod photoreceptors and cone outer segments.

Description:

This gene encodes a protein with a series of six RCC1-like domains (RLDs), characteristic of the highly conserved guanine nucleotide exchange factors. The encoded protein is found in the Golgi body and interacts with RPGRIP1. This protein localizes to the outer segment of rod photoreceptors and is essential for their viability. Mutations in this gene have been associated with X-linked retinitis pigmentosa (XLRP). Multiple alternatively spliced transcript variants that encode different isoforms of this gene have been reported, but the full-length natures of only some have been determined. [provided by RefSeq, Dec 2008]

Sequence:

Q92834 RPGR_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MREPEELMPDSGAVFTFGKSKFAENNPGKFWFKNDVPVHLSCGDEHSAVVTGNNKLYMFGSNNWGQLGLGSKSAISKPTCVKALKPEKVKLAACGRNHTLVSTEGGNVYATGGNNEGQLGLGDTEERNTFHVISFFTSEHKIKQLSAGSNTSAALTEDGRLFMWGDNSEGQIGLKNVSNVCVPQQVTIGKPVSWISCGYYHSAFVTTDGELYVFGEPENGKLGLPNQLLGNHRTPQLVSEIPEKVIQVACGGEHTVVLTENAVYTFGLGQFGQLGLGTFLFETSEPKVIENIRDQTISYISCGENHTALITDIGLMYTFGDGRHGKLGLGLENFTNHFIPTLCSNFLRFIVKLVACGGCHMVVFAAPHRGVAKEIEFDEINDTCLSVATFLPYSSLTSGNVLQRTLSARMRRRERERSPDSFSMRRTLPPIEGTLGLSACFLPNSVFPRCSERNLQESVLSEQDLMQPEEPDYLLDEMTKEAEIDNSSTVESLGETTDILNMTHIMSLNSNEKSLKLSPVQKQKKQQTIGELTQDTALTENDDSDEYEEMSEMKEGKACKQHVSQGIFMTQPATTIEAFSDEEVGNDTGQVGPQADTDGEGLQKEVYRHENNNGVDQLDAKEIEKESDGGHSQKESEAEEIDSEKETKLAEIAGMKDLREREKSTKKMSPFFGNLPDRGMNTESEENKDFVKKRESCKQDVIFDSERESVEKPDSYMEGASESQQGIADGFQQPEAIEFSSGEKEDDEVETDQNIRYGRKLIEQGNEKETKPIISKSMAKYDFKCDRLSEIPEEKEGAEDSKGNGIEEQEVEANEENVKVHGGRKEKTEILSDDLTDKAEDHEFSKTEELKLEDVDEEINAENVESKKKTVGDDESVPTGYHSKTEGAERTNDDSSAETIEKKEKANLEERAICEYNENPKGYMLDDADSSSLEILENSETTPSKDMKKTKKIFLFKRVPSINQKIVKNNNEPLPEIKSIGDQIILKSDNKDADQNHMSQNHQNIPPTNTERRSKSCTIL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Dog

Horse

Bovine

Sheep

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92834 As Substrate

Q92834

Site	PTM Type	Source
K143	Ubiquitination	Uniprot
T335	Phosphorylation	Uniprot
Y393	Phosphorylation	Uniprot
S418	Phosphorylation	Uniprot
S514	Phosphorylation	Uniprot
S518	Phosphorylation	Uniprot
S544	Phosphorylation	Uniprot
S866	Phosphorylation	Uniprot
T870	Phosphorylation	Uniprot
S876	Phosphorylation	Uniprot
S883	Phosphorylation	Uniprot
T891	Phosphorylation	Uniprot
Y916	Phosphorylation	Uniprot
S961	Phosphorylation	Uniprot

Research Backgrounds

Q92834_RPGR_HUMAN

Function:

Could be a guanine-nucleotide releasing factor. Plays a role in ciliogenesis. Probably regulates cilia formation by regulating actin stress filaments and cell contractility. Plays an important role in photoreceptor integrity. May play a critical role in spermatogenesis and in intraflagellar transport processes (By similarity). May be involved in microtubule organization and regulation of transport in primary cilia.

PTMs:

Prenylated.

Subcellular Location:

Cytoplasm>Cytoskeleton>Flagellum axoneme. Golgi apparatus. Cell projection>Cilium.
Note: In the retinal photoreceptor cell layer, localizes at the connecting cilium (By similarity). Colocalizes with WHRN in the photoreceptor connecting cilium (By similarity). Colocalizes with CEP290 in the photoreceptor connecting cilium (By similarity). Colocalizes with RPGRIP1 in the photoreceptor connecting cilium (By similarity).

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Cilium basal body. Cytoplasm>Cytoskeleton>Cilium axoneme.

Tissue Specificity:

Subunit Structure:

Interacts with SPATA7 (By similarity). Interacts with CEP290 (By similarity). Interacts with WHRN (By similarity). Interacts with PDE6D. Interacts with RPGRIP1. Interacts with RPGRIP1L. PDE6D, RPGRIP1 and RPGRIP1L may compete for the same binding sites. Isoform 6 interacts with NPM1 (via C-terminus). Isoform 6 interacts with SMC1A and SMC3.

Family&Domains:

The RCC1 repeat region mediates interactions with RPGRIP1.

Restrictive clause

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RPGR Antibody - #DF6654