AMBP Antibody | Affinity Biosciences

1/2

AMBP Antibody - Western blot analysis of extracts from Mouse brain, using AMBP Antibody.

AMBP Antibody - DF6656 at 1/100 staining Mouse testis tissue by IHC-P.

Product:	AMBP Antibody
Catalog:	DF6656
Description:	Rabbit polyclonal antibody to AMBP
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	39kDa; 39kD(Calculated).
Uniprot:	P02760
RRID:	AB_2838618

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(90%), Zebrafish(80%), Bovine(90%), Horse(90%), Sheep(80%), Rabbit(100%), Dog(100%), Chicken(90%), Xenopus(80%)

Clonality:

Polyclonal

Specificity:

AMBP Antibody detects endogenous levels of total AMBP.

RRID:

AB_2838618
Cite Format: Affinity Biosciences Cat# DF6656, RRID:AB_2838618.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

A1M; Alpha 1 microglobulin/bikunin precursor; Alpha 1 microglycoprotein; Alpha-1 microglycoprotein; Alpha-1-microglobulin; AMBP; AMBP_HUMAN; Bikunin; Complex-forming glycoprotein heterogeneous in charge; EDC1; Growth inhibiting protein 19; HCP; HI-30; HI30; IATIL; ITI; ITI-LC; ITIL; Microglobulin (alpha 1); Protein AMBP; Protein HC; Trypstatin; Uristatin; Uronic-acid-rich protein; UTI;

Immunogens

Immunogen:

Uniprot:

P02760(AMBP_HUMAN) >>Visit HPA database.

Gene(ID):

AMBP(259)

Expression:

P02760 AMBP_HUMAN:

Expressed by the liver and secreted in plasma. Alpha-1-microglobulin occurs in many physiological fluids including plasma, urine, and cerebrospinal fluid. Inter-alpha-trypsin inhibitor is present in plasma and urine.

Description:

This gene encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes.

Sequence:

P02760 AMBP_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MRSLGALLLLLSACLAVSAGPVPTPPDNIQVQENFNISRIYGKWYNLAIGSTCPWLKKIMDRMTVSTLVLGEGATEAEISMTSTRWRKGVCEETSGAYEKTDTDGKFLYHKSKWNITMESYVVHTNYDEYAIFLTKKFSRHHGPTITAKLYGRAPQLRETLLQDFRVVAQGVGIPEDSIFTMADRGECVPGEQEPEPILIPRVRRAVLPQEEEGSGGGQLVTEVTKKEDSCQLGYSAGPCMGMTSRYFYNGTSMACETFQYGGCMGNGNNFVTEKECLQTCRTVAACNLPIVRGPCRAFIQLWAFDAVKGKCVLFPYGGCQGNGNKFYSEKECREYCGVPGDGDEELLRFSN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Dog

100

Pig

Horse

Bovine

Chicken

Sheep

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P02760 As Substrate

P02760

Site	PTM Type	Source
N36	N-Glycosylation	Uniprot
S66	Phosphorylation	Uniprot
T75	Phosphorylation	Uniprot
N115	N-Glycosylation	Uniprot
S215	O-Glycosylation	Uniprot
N250	N-Glycosylation	Uniprot

Research Backgrounds

P02760_AMBP_HUMAN

Function:

Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization.

Trypstatin is a trypsin inhibitor.

PTMs:

The precursor is proteolytically processed into separately functioning proteins.

3-hydroxykynurenine, an oxidized tryptophan metabolite that is common in biological fluids, reacts with Cys-53, Lys-111, Lys-137, and Lys-149 to form heterogeneous polycyclic chromophores including hydroxanthommatin. The reaction by alpha-1-microglobulin is autocatalytic; the human protein forms chromophore even when expressed in insect and bacterial cells. The chromophore can react with accessible cysteines forming non-reducible thioether cross-links with other molecules of alpha-1-microglobulin or with other proteins such as Ig alpha-1 chain C region 'Cys-352'.

Heavy chains are interlinked with bikunin via a chondroitin 4-sulfate bridge to the their C-terminal aspartate.

N- and O-glycosylated. N-glycan heterogeneity at Asn-115: Hex5HexNAc4 (major), Hex6HexNAc5 (minor) and dHex1Hex6HexNAc5 (minor). N-glycan at Asn-250: Hex5HexNAc4. O-linkage of the glycosaminoglycan, chondroitin sulfate, at Ser-215 allows cross-linking between the three polypeptide chains.

Subcellular Location:

Secreted.

Tissue Specificity:

Subunit Structure:

I-alpha-I plasma protease inhibitors are assembled from one or two heavy chains (H1, H2 or H3) and one light chain, bikunin. Inter-alpha-inhibitor (I-alpha-I) is composed of H1, H2 and bikunin, inter-alpha-like inhibitor (I-alpha-LI) of H2 and bikunin, and pre-alpha-inhibitor (P-alpha-I) of H3 and bikunin. Alpha-1-microglobulin occurs as a monomer and also in complexes with IgA and albumin. Alpha-1-microglobulin interacts with FN1. Trypstatin is a monomer and also occurs as a complex with tryptase in mast cells (By similarity).

(Microbial infection) Alpha-1-microglobulin and bikunin interact (via SH3 domain) with hepatitis E virus/HEV protein ORF3.

Family&Domains:

In the N-terminal section; belongs to the calycin superfamily. Lipocalin family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

AMBP Antibody - #DF6656