CADM1 Antibody - #DF6679

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Product: CADM1 Antibody
Catalog: DF6679
Description: Rabbit polyclonal antibody to CADM1
Application: WB IHC IF/ICC
Cited expt.: IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 49kDa; 49kD(Calculated).
Uniprot: Q9BY67
RRID: AB_2838641

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(91%), Xenopus(82%)
Clonality:
Polyclonal
Specificity:
CADM1 Antibody detects endogenous levels of total CADM1.
RRID:
AB_2838641
Cite Format: Affinity Biosciences Cat# DF6679, RRID:AB_2838641.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BL2; Cadm1; CADM1_HUMAN; Cell adhesion molecule 1; DKFZp686F1789; IgSF4; IGSF4A; Immunoglobulin superfamily member 4; MGC149785; MGC51880; NECL-2; NECL2; Nectin like 2; Nectin-like protein 2; RA175; SgIGSF; Spermatogenic immunoglobulin superfamily; ST17; sTSLC 1; Synaptic cell adhesion molecule; SynCAM; SynCAM1; TSLC-1; TSLC1; Tumor suppressor in lung cancer 1;

Immunogens

Immunogen:

A synthesized peptide derived from human CADM1, corresponding to a region within the internal amino acids.

Uniprot:

Q9BY67(CADM1_HUMAN) >>Visit HPA database.

Gene(ID):
CADM1(23705)
Description:
Homologous to the poliovirus receptor (PVR/CD155), the Nectin immunoglobulin superfamily comprises four known isoforms, Nectin 1, 2, 3 and 4 (also designated TSLC1). TSLC1 is encoded by a tumor-suppressor gene in human non-small-cell lung cancer mapping to chromosome 11q23.2. The TSLC1 protein is an N-linked membrane glycoprotein that co-localizes with the Actin filament-binding protein, afadin, at cadherin-based adherens junctions in MDCKII epithelial cells. TSLC1 also interacts with the tumor-suppressor gene product DAL-1 (for differentially expressed in adenocarcinoma of the lung protein 1) to target Actin rearragement and cellular motility. TSLC1 may also form homodimers that function in homophilic, intracellular adhesion. TSLC1 expression is reduced or absent in a number of characterized cancer cell lines including A549. In prostate and breast cancer, as well as in pancreatic ductal adenocarcinoma, the TSLC1 promoter is commonly silenced by hypermethylation. Unlike other Nectins, which are more widely expressed, TSLC1 is mainly expressed in the placenta.
Sequence:
MASVVLPSGSQCAAAAAAAAPPGLRLRLLLLLFSAAALIPTGDGQNLFTKDVTVIEGEVATISCQVNKSDDSVIQLLNPNRQTIYFRDFRPLKDSRFQLLNFSSSELKVSLTNVSISDEGRYFCQLYTDPPQESYTTITVLVPPRNLMIDIQKDTAVEGEEIEVNCTAMASKPATTIRWFKGNTELKGKSEVEEWSDMYTVTSQLMLKVHKEDDGVPVICQVEHPAVTGNLQTQRYLEVQYKPQVHIQMTYPLQGLTREGDALELTCEAIGKPQPVMVTWVRVDDEMPQHAVLSGPNLFINNLNKTDNGTYRCEASNIVGKAHSDYMLYVYDPPTTIPPPTTTTTTTTTTTTTILTIITDSRAGEEGSIRAVDHAVIGGVVAVVVFAMLCLLIILGRYFARHKGTYFTHEAKGADDAADADTAIINAEGGQNNSEEKKEYFI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
91
Xenopus
82
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Mediates homophilic cell-cell adhesion in a Ca(2+)-independent manner. Also mediates heterophilic cell-cell adhesion with CADM3 and NECTIN3 in a Ca(2+)-independent manner. Acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells. Interaction with CRTAM promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo. May contribute to the less invasive phenotypes of lepidic growth tumor cells. In mast cells, may mediate attachment to and promote communication with nerves. CADM1, together with MITF, is essential for development and survival of mast cells in vivo. Acts as a synaptic cell adhesion molecule and plays a role in the formation of dendritic spines and in synapse assembly (By similarity). May be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons. May play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa.

PTMs:

Glycosylation at Asn-67 and Asn-101 promotes adhesive binding and synapse induction.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein. Cell junction>Synapse.
Note: Localized to the basolateral plasma membrane of epithelial cells in gall bladder.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

The cytoplasmic domain appears to play a critical role in proapoptosis and tumor suppressor activity in NSCLC.

Belongs to the nectin family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

References

1). Single-cell sequencing reveals glial cell involvement in development of neuropathic pain via myelin sheath lesion formation in the spinal cord. Journal of neuroinflammation, 2024 (PubMed: 39217340) [IF=9.3]

Application: IF/ICC    Species: Mouse    Sample: spinal cord

Fig. 10 From: Single-cell sequencing reveals glial cell involvement in development of neuropathic pain via myelin sheath lesion formation in the spinal cord Analysis of receptor–ligand interactions among spinal cord glial cells in CON and CCI groups. Bubble diagram showing differential expression patterns of receptor–ligand interaction pairs between astrocyte and oligodendrocyte subpopulations in the CON and CCI groups: (A) Astrocyte ligands, (B) Oligodendrocyte ligands. (C) Bar plots showing GO pathway annotation in astrocytes in the CON and CCI groups. (D-F) Bubble diagram showing differential expression patterns of receptor–ligand interaction pairs between microglia and oligodendrocyte subpopulations in the CON and CCI groups. Ligands are Microglia-1, -2 and − 8. Receptors are oligodendrocytes. (G) UMAP plots showing distributions of differentially expressed marker genes in spinal cord tissue. (H) Bubble plots showing expression status and interactions of receptor–ligand pairs of glial cell subsets in the CON and CCI groups. (I) Heatmap showing interactions between microglia and astrocyte subpopulations in the CON and CCI groups. (J) Bubble diagram showing expression status and interactions of receptor–ligand pairs for microglia and astrocytes in the CON and CCI groups. (K) Representative images of IF staining for CADM1 in spinal cord tissue of the CON and CCI groups. From left to right: CADM1, CADM1 channel image; DAPI, DAPI channel image: Merge, merged image between CADM1 and DAPI. Scale bar = 100 μm. n = 3; 3 sections per animal. (L) Representative VEGFA and NRP-1 staining images of spinal cord sections from the CON and CCI groups. From left to right: VEGFA, VEGFA channel image; NRP-1, NRP-1 channel image; DAPI, DAPI channel image; Merge, merged image among VEGFA, NRP-1 and DAPI. Scale bar = 100 μm. n = 5. (M) Bar graphs showing quantification of fluorescent images of CADM1, NRP1 and VEGFA as normalized within the CON and CCI groups. Data are presented as means ± SEMs. ###, P 

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