PADI4 Antibody - #DF6685
| Product: | PADI4 Antibody |
| Catalog: | DF6685 |
| Description: | Rabbit polyclonal antibody to PADI4 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Rabbit |
| Mol.Wt.: | 74kDa; 74kD(Calculated). |
| Uniprot: | Q9UM07 |
| RRID: | AB_2838647 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF6685, RRID:AB_2838647.
Fold/Unfold
EC 3.5.3.15; HL 60 PAD; HL-60 PAD; PAD 4; PAD; PADI 4; PADI 5; PADI H protein; Padi4; PADI4_HUMAN; PADI5; PDI 4; PDI 5; PDI4; PDI5; Peptidyl arginine deiminase type IV; Peptidyl arginine deiminase type V; Peptidylarginine deiminase IV; Protein arginine deiminase; Protein arginine deiminase type 4; Protein arginine deiminase type IV; Protein-arginine deiminase type IV; Protein-arginine deiminase type-4;
Immunogens
Expressed in eosinophils and neutrophils, not expressed in peripheral monocytes or lymphocytes.
- Q9UM07 PADI4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAQGTLIRVTPEQPTHAVCVLGTLTQLDICSSAPEDCTSFSINASPGVVVDIAHGPPAKKKSTGSSTWPLDPGVEVTLTMKVASGSTGDQKVQISYYGPKTPPVKALLYLTGVEISLCADITRTGKVKPTRAVKDQRTWTWGPCGQGAILLVNCDRDNLESSAMDCEDDEVLDSEDLQDMSLMTLSTKTPKDFFTNHTLVLHVARSEMDKVRVFQATRGKLSSKCSVVLGPKWPSHYLMVPGGKHNMDFYVEALAFPDTDFPGLITLTISLLDTSNLELPEAVVFQDSVVFRVAPWIMTPNTQPPQEVYACSIFENEDFLKSVTTLAMKAKCKLTICPEEENMDDQWMQDEMEIGYIQAPHKTLPVVFDSPRNRGLKEFPIKRVMGPDFGYVTRGPQTGGISGLDSFGNLEVSPPVTVRGKEYPLGRILFGDSCYPSNDSRQMHQALQDFLSAQQVQAPVKLYSDWLSVGHVDEFLSFVPAPDRKGFRLLLASPRSCYKLFQEQQNEGHGEALLFEGIKKKKQQKIKNILSNKTLREHNSFVERCIDWNRELLKRELGLAESDIIDIPQLFKLKEFSKAEAFFPNMVNMLVLGKHLGIPKPFGPVINGRCCLEEKVCSLLEPLGLQCTFINDFFTYHIRHGEVHCGTNVRRKPFSFKWWNMVP
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9UM07 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T63 | Phosphorylation | Uniprot | |
| T217 | Phosphorylation | Uniprot | |
| S222 | Phosphorylation | Uniprot | |
| T363 | Phosphorylation | Uniprot | |
| S402 | Phosphorylation | Uniprot | |
| S406 | Phosphorylation | Uniprot | |
| S493 | Phosphorylation | Uniprot | |
| K533 | Acetylation | Uniprot |
Research Backgrounds
Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci). Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1: citrullination of 'Arg-54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance. Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci. Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1.
Autocitrullination at Arg-372 and Arg-374 inactivates the enzyme.
Cytoplasm. Nucleus. Cytoplasmic granule.
Note: Cytoplasmic granules of eosinophils and neutrophils.
Expressed in eosinophils and neutrophils, not expressed in peripheral monocytes or lymphocytes.
Belongs to the protein arginine deiminase family.
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