CD151 Antibody - #DF6699

(3)  
Product: CD151 Antibody
Catalog: DF6699
Description: Rabbit polyclonal antibody to CD151
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Dog, Xenopus
Mol.Wt.: 28kDa; 28kD(Calculated).
Uniprot: P48509
RRID: AB_2838661

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(80%), Horse(100%), Dog(90%), Xenopus(90%)
Clonality:
Polyclonal
Specificity:
CD151 Antibody detects endogenous levels of total CD151.
RRID:
AB_2838661
Cite Format: Affinity Biosciences Cat# DF6699, RRID:AB_2838661.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CD151; CD151 antigen; CD151 molecule (Raph blood group); CD151 molecule; CD151_HUMAN; GP27; hemidesmosomal tetraspanin CD151; Membrane glycoprotein SFA-1; MER2; PETA-3; PETA3; Platelet endothelial tetraspan antigen 3; platelet surface glycoprotein gp27; Platelet-endothelial cell tetraspan antigen 3; Platelet-endothelial tetraspan antigen 3; RAPH; Red blood cell antigen MER2; SFA 1; SFA1; Tetraspanin-24; Tspan-24; TSPAN24;

Immunogens

Immunogen:
Uniprot:

P48509(CD151_HUMAN) >>Visit HPA database.

Gene(ID):
CD151(977)
Expression:
P48509 CD151_HUMAN:

Expressed in a variety of tissues including vascular endothelium and epidermis. Expressed on erythroid cells, with a higher level of expression in erythroid precursors than on mature erythrocytes.

Description:
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It is involved in cellular processes including cell adhesion and may regulate integrin trafficking and/or function. This protein enhances cell motility, invasion and metastasis of cancer cells. Multiple alternatively spliced transcript variants that encode the same protein have been described for this gene.
Sequence:
MGEFNEKKTTCGTVCLKYLLFTYNCCFWLAGLAVMAVGIWTLALKSDYISLLASGTYLATAYILVVAGTVVMVTGVLGCCATFKERRNLLRLYFILLLIIFLLEIIAGILAYAYYQQLNTELKENLKDTMTKRYHQPGHEAVTSAVDQLQQEFHCCGSNNSQDWRDSEWIRSQEAGGRVVPDSCCKTVVALCGQRDHASNIYKVEGGCITKLETFIQEHLRVIGAVGIGIACVQVFGMIFTCCLYRSLKLEHY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
90
Xenopus
90
Bovine
80
Chicken
70
Pig
60
Sheep
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P48509 As Substrate

Site PTM Type Enzyme
K7 Ubiquitination
K8 Ubiquitination
K17 Ubiquitination
Y48 Phosphorylation
K132 Ubiquitination
K186 Ubiquitination
S199 Phosphorylation
K203 Ubiquitination
K249 Ubiquitination
Y253 Phosphorylation

Research Backgrounds

Function:

Essential for the proper assembly of the glomerular and tubular basement membranes in kidney.

(Microbial infection) Plays a role in human papillomavirus 16/HPV-16 endocytosis upon binding to cell surface receptor.

Subcellular Location:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in a variety of tissues including vascular endothelium and epidermis. Expressed on erythroid cells, with a higher level of expression in erythroid precursors than on mature erythrocytes.

Subunit Structure:

Interacts with integrins ITGA3:ITGB1, ITGA5:ITGB1, ITGA3:ITGB1 and ITGA6:ITGB4 and with CD9 and CD181. Interacts (via the second extracellular domain) with integrin ITGAV:ITGB3.

Family&Domains:

Belongs to the tetraspanin (TM4SF) family.

Restrictive clause

 

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For Research Use Only.
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