MSMB Antibody - #DF6720

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Product: MSMB Antibody
Catalog: DF6720
Description: Rabbit polyclonal antibody to MSMB
Application: WB IHC
Cited expt.: WB
Reactivity: Human, Mouse
Mol.Wt.: 13kDa; 13kD(Calculated).
Uniprot: P08118
RRID: AB_2838682

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Clonality:
Polyclonal
Specificity:
MSMB Antibody detects endogenous levels of total MSMB.
RRID:
AB_2838682
Cite Format: Affinity Biosciences Cat# DF6720, RRID:AB_2838682.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Beta microseminoprotein [Precursor]; Beta-microseminoprotein; HPC 13; HPC13; IGBF; Immunoglobulin binding factor; Immunoglobulin-binding factor; microseminoprotein beta; Msmb; MSMB_HUMAN; MSP; MSPB; PN 44; PN44; Prostate secreted seminal plasma protein; Prostate secretory protein of 94 amino acids; Prostate secretory protein PSP94; PRPS; PRSP; PSP 57; PSP; PSP-94; PSP57; PSP94; Seminal plasma beta inhibin; Seminal plasma beta-inhibin;

Immunogens

Immunogen:

A synthesized peptide derived from human MSMB, corresponding to a region within the internal amino acids.

Uniprot:

P08118(MSMB_HUMAN) >>Visit HPA database.

Gene(ID):
MSMB(4477)
Expression:
P08118 MSMB_HUMAN:

Strongly expressed in prostate, liver, kidney, breast and penis. Also expressed in pancreas, esophagus, stomach, deodenum, colon, trachea, lung, salivary glands and fallopian tube. PSP94 is expressed in lung and breast, whereas PSP57 is found in kidney and bladder.

Description:
The protein encoded by this gene is a member of the immunoglobulin binding factor family. It is synthesized by the epithelial cells of the prostate gland and secreted into the seminal plasma. This protein has inhibin-like activity. It may have a role as an autocrine paracrine factor in uterine, breast and other female reproductive tissues. The expression of the encoded protein is found to be decreased in prostate cancer. Two alternatively spliced transcript variants encoding different isoforms are described for this gene. The use of alternate polyadenylation sites has been found for this gene.
Sequence:
MNVLLGSVVIFATFVTLCNASCYFIPNEGVPGDSTRKCMDLKGNKHPINSEWQTDNCETCTCYETEISCCTLVSTPVGYDKDNCQRIFKKEDCKYIVVEKKDPKKTCSVSEWII

Research Backgrounds

Subcellular Location:

Secreted.
Note: Sperm surface.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Strongly expressed in prostate, liver, kidney, breast and penis. Also expressed in pancreas, esophagus, stomach, deodenum, colon, trachea, lung, salivary glands and fallopian tube. PSP94 is expressed in lung and breast, whereas PSP57 is found in kidney and bladder.

Family&Domains:

Belongs to the beta-microseminoprotein family.

References

1). Identification and validation of MSMB as a critical gene for prostate cancer development in obese people. American journal of cancer research, 2023 (PubMed: 37168344) [IF=3.6]

Application: WB    Species: human    Sample:

Figure 4. MSMB downregulated in obese PCA patients and influence PCA cells proliferation. The mRNA (A) and protein (B) level of MSMB between PCA tumor tissues and adjacent normal tissues in obese PCA patients. (C, D) The protein level of MSMB in two types of PCA cell lines: PC-3 (C) and DU145 (D) after PCA cells transfected by oeMSMB plasmids or not. Cell proliferation of PC-3 (E) and DU145 (F) was detected after cells transfected by oeControl and oeMSMB plasmids. *represents P < 0.05. **represents P < 0.01. The RT-qPCR and Western blot used GAPDH as endogenous control. N: normal tissues, T: tumor tissues.

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