SMARCA5 Antibody - #DF6744

(1)  
Product: SMARCA5 Antibody
Catalog: DF6744
Description: Rabbit polyclonal antibody to SMARCA5
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 122kDa; 122kD(Calculated).
Uniprot: O60264
RRID: AB_2838706

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
SMARCA5 Antibody detects endogenous levels of total SMARCA5.
RRID:
AB_2838706
Cite Format: Affinity Biosciences Cat# DF6744, RRID:AB_2838706.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

4933427E24Rik; D030040M08Rik; D330027N15Rik; EC 3.6.1.-; hISWI; hSNF2H; ISWI; MommeD4; SMARCA5; SMCA5_HUMAN; Snf2h; Sucrose nonfermenting like 5; Sucrose nonfermenting protein 2 homolog; Sucrose nonfermenting, yeast, homolog of; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5; WCRF135;

Immunogens

Immunogen:
Uniprot:

O60264(SMCA5_HUMAN) >>Visit HPA database.

Gene(ID):
SMARCA5(8467)
Expression:
O60264 SMCA5_HUMAN:

Ubiquitously expressed.

Description:
The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The protein encoded by this gene is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II. The encoded protein is similar in sequence to the Drosophila ISWI chromatin remodeling protein.
Sequence:
MSSAAEPPPPPPPESAPSKPAASIASGGSNSSNKGGPEGVAAQAVASAASAGPADAEMEEIFDDASPGKQKEIQEPDPTYEEKMQTDRANRFEYLLKQTELFAHFIQPAAQKTPTSPLKMKPGRPRIKKDEKQNLLSVGDYRHRRTEQEEDEELLTESSKATNVCTRFEDSPSYVKWGKLRDYQVRGLNWLISLYENGINGILADEMGLGKTLQTISLLGYMKHYRNIPGPHMVLVPKSTLHNWMSEFKRWVPTLRSVCLIGDKEQRAAFVRDVLLPGEWDVCVTSYEMLIKEKSVFKKFNWRYLVIDEAHRIKNEKSKLSEIVREFKTTNRLLLTGTPLQNNLHELWSLLNFLLPDVFNSADDFDSWFDTNNCLGDQKLVERLHMVLRPFLLRRIKADVEKSLPPKKEVKIYVGLSKMQREWYTRILMKDIDILNSAGKMDKMRLLNILMQLRKCCNHPYLFDGAEPGPPYTTDMHLVTNSGKMVVLDKLLPKLKEQGSRVLIFSQMTRVLDILEDYCMWRNYEYCRLDGQTPHDERQDSINAYNEPNSTKFVFMLSTRAGGLGINLATADVVILYDSDWNPQVDLQAMDRAHRIGQTKTVRVFRFITDNTVEERIVERAEMKLRLDSIVIQQGRLVDQNLNKIGKDEMLQMIRHGATHVFASKESEITDEDIDGILERGAKKTAEMNEKLSKMGESSLRNFTMDTESSVYNFEGEDYREKQKIAFTEWIEPPKRERKANYAVDAYFREALRVSEPKAPKAPRPPKQPNVQDFQFFPPRLFELLEKEILFYRKTIGYKVPRNPELPNAAQAQKEEQLKIDEAESLNDEELEEKEKLLTQGFTNWNKRDFNQFIKANEKWGRDDIENIAREVEGKTPEEVIEYSAVFWERCNELQDIEKIMAQIERGEARIQRRISIKKALDTKIGRYKAPFHQLRISYGTNKGKNYTEEEDRFLICMLHKLGFDKENVYDELRQCIRNSPQFRFDWFLKSRTAMELQRRCNTLITLIERENMELEEKEKAEKKKRGPKPSTQKRKMDGAPDGRGRKKKLKL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
75
Zebrafish
75
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O60264 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
S23 Phosphorylation
S26 Phosphorylation
S29 Phosphorylation
S50 Phosphorylation
S66 Phosphorylation
K71 Ubiquitination
Y80 Phosphorylation
K83 Sumoylation
K83 Ubiquitination
T113 Phosphorylation
T115 Phosphorylation
S116 Phosphorylation
K129 Acetylation
K132 Acetylation
K132 Ubiquitination
S137 Phosphorylation
Y141 Phosphorylation
T156 Phosphorylation
K160 Ubiquitination
S171 Phosphorylation
K176 Ubiquitination
K249 Acetylation
T254 Phosphorylation
S257 Phosphorylation
K264 Acetylation
K264 Ubiquitination
K319 Ubiquitination
K397 Acetylation
K402 Acetylation
K418 Ubiquitination
K430 Acetylation
K430 Ubiquitination
S437 Phosphorylation
K440 Acetylation
K440 Ubiquitination
K490 Ubiquitination
K600 Acetylation
K600 Ubiquitination
K644 Ubiquitination
K647 Acetylation
K647 Sumoylation
K647 Ubiquitination
K665 Ubiquitination
T670 Phosphorylation
K683 Acetylation
K683 Ubiquitination
T685 Phosphorylation
K691 Ubiquitination
K694 Sumoylation
K694 Ubiquitination
S698 Phosphorylation
S699 Phosphorylation
Y712 Phosphorylation
Y719 Phosphorylation
K724 Ubiquitination
K735 Ubiquitination
K739 Sumoylation
K739 Ubiquitination
Y742 Phosphorylation
Y747 Phosphorylation
S755 Phosphorylation
Y798 Phosphorylation
K799 Ubiquitination
K814 Ubiquitination
S825 Phosphorylation
K834 Ubiquitination
K836 Ubiquitination
K847 Ubiquitination
K855 Ubiquitination
T923 Phosphorylation
K924 Ubiquitination
K929 Sumoylation
K929 Ubiquitination
K945 Ubiquitination
Y947 Phosphorylation
K961 Ubiquitination
Y970 Phosphorylation
R974 Methylation
K1018 Ubiquitination
R1044 Methylation
K1049 Acetylation

Research Backgrounds

Function:

Helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity. Complexes containing SMARCA5 are capable of forming ordered nucleosome arrays on chromatin; this may require intact histone H4 tails. Also required for replication of pericentric heterochromatin in S-phase specifically in conjunction with BAZ1A. Probably plays a role in repression of polI dependent transcription of the rDNA locus, through the recruitment of the SIN3/HDAC1 corepressor complex to the rDNA promoter. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes. Essential component of the NoRC (nucleolar remodeling complex) complex, a complex that mediates silencing of a fraction of rDNA by recruiting histone-modifying enzymes and DNA methyltransferases, leading to heterochromatin formation and transcriptional silencing.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed.

Subunit Structure:

Catalytic subunit of the four known chromatin-remodeling complexes: CHRAC, RSF, ACF/WCRF, and WICH. Each complex contains subunits which may regulate the specificity or catalytic activity of SMARCA5. ACF/WCRF contains BAZ1A; CHRAC contains BAZ1A, CHRAC1, and POLE3; RSF contains RSF1; WICH contains BAZ1B/WSTF. SMARCA5 is the catalytic subunit of the NoRC chromatin-remodeling complex, which also contains BAZ2A/TIP5. The BAZ2A/TIP5 subunit of NoRC also interacts with DNMT1, DNMT3B and HDAC1, which allows NoRC to actively suppress rDNA transcription by a combination of nucleosome remodeling, histone deacetylation, and DNA methylation. Catalytic subunit of SMARCA5/cohesin/NuRD complexes. Component of the B-WICH complex, at least composed of SMARCA5/SNF2H, BAZ1B/WSTF, SF3B1, DEK, MYO1C, ERCC6, MYBBP1A and DDX21. Interacts with MYO1C (By similarity). Interacts with BEND3.

Family&Domains:

Belongs to the SNF2/RAD54 helicase family. ISWI subfamily.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.