POSTN Antibody - #DF6746

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Product: POSTN Antibody
Catalog: DF6746
Description: Rabbit polyclonal antibody to POSTN
Application: WB IHC
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Chicken
Mol.Wt.: 93kDa; 93kD(Calculated).
Uniprot: Q15063
RRID: AB_2838708

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(91%), Bovine(82%), Horse(82%), Sheep(82%), Rabbit(91%), Chicken(82%)
Clonality:
Polyclonal
Specificity:
POSTN Antibody detects endogenous levels of total POSTN.
RRID:
AB_2838708
Cite Format: Affinity Biosciences Cat# DF6746, RRID:AB_2838708.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Fasciclin-I like; MGC119510; MGC119511; OSF 2; OSF-2; OSF2; Osteoblast specific factor 2 (fasciclin I like); Osteoblast specific factor 2; Osteoblast specific factor; Osteoblast-specific factor 2; PDLPOSTN; Periodontal ligament specific periostin; Periostin; Periostin isoform thy2; Periostin isoform thy4; Periostin isoform thy6; Periostin isoform thy8; Periostin osteoblast specific factor; PN; POSTN; POSTN_HUMAN; RP11 412K4.1;

Immunogens

Immunogen:

A synthesized peptide derived from human POSTN, corresponding to a region within N-terminal amino acids.

Uniprot:

Q15063(POSTN_HUMAN) >>Visit HPA database.

Gene(ID):
POSTN(10631)
Expression:
Q15063 POSTN_HUMAN:

Widely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus, thyroid tissue and breast. Up-regulated in epithelial ovarian tumors. Not expressed in normal ovaries. Also highly expressed at the tumor periphery of lung carcinoma tissue but not within the tumor. Overexpressed in breast cancers.

Sequence:
MIPFLPMFSLLLLLIVNPINANNHYDKILAHSRIRGRDQGPNVCALQQILGTKKKYFSTCKNWYKKSICGQKTTVLYECCPGYMRMEGMKGCPAVLPIDHVYGTLGIVGATTTQRYSDASKLREEIEGKGSFTYFAPSNEAWDNLDSDIRRGLESNVNVELLNALHSHMINKRMLTKDLKNGMIIPSMYNNLGLFINHYPNGVVTVNCARIIHGNQIATNGVVHVIDRVLTQIGTSIQDFIEAEDDLSSFRAAAITSDILEALGRDGHFTLFAPTNEAFEKLPRGVLERIMGDKVASEALMKYHILNTLQCSESIMGGAVFETLEGNTIEIGCDGDSITVNGIKMVNKKDIVTNNGVIHLIDQVLIPDSAKQVIELAGKQQTTFTDLVAQLGLASALRPDGEYTLLAPVNNAFSDDTLSMDQRLLKLILQNHILKVKVGLNELYNGQILETIGGKQLRVFVYRTAVCIENSCMEKGSKQGRNGAIHIFREIIKPAEKSLHEKLKQDKRFSTFLSLLEAADLKELLTQPGDWTLFVPTNDAFKGMTSEEKEILIRDKNALQNIILYHLTPGVFIGKGFEPGVTNILKTTQGSKIFLKEVNDTLLVNELKSKESDIMTTNGVIHVVDKLLYPADTPVGNDQLLEILNKLIKYIQIKFVRGSTFKEIPVTVYTTKIITKVVEPKIKVIEGSLQPIIKTEGPTLTKVKIEGEPEFRLIKEGETITEVIHGEPIIKKYTKIIDGVPVEITEKETREERIITGPEIKYTRISTGGGETEETLKKLLQEEVTKVTKFIEGGDGHLFEDEEIKRLLQGDTPVRKLQANKKVQGSRRRLREGRSQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
91
Pig
91
Horse
82
Bovine
82
Sheep
82
Chicken
82
Dog
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Induces cell attachment and spreading and plays a role in cell adhesion. Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX (By similarity).

PTMs:

Gamma-carboxylation is controversial. Gamma-carboxyglutamated; gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation; this may be required for calcium binding. According to a more recent report, does not contain vitamin K-dependent gamma-carboxyglutamate residues.

Subcellular Location:

Golgi apparatus. Secreted. Secreted>Extracellular space>Extracellular matrix.
Note: Colocalizes with BMP1 in the Golgi.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus, thyroid tissue and breast. Up-regulated in epithelial ovarian tumors. Not expressed in normal ovaries. Also highly expressed at the tumor periphery of lung carcinoma tissue but not within the tumor. Overexpressed in breast cancers.

References

1). Neuropeptide Precursor VGF Promotes Neuroendocrine Differentiation and Cancer-Associated Fibroblast Activation in Small Cell Lung Cancer. Cancer research, 2025 (PubMed: 40857617) [IF=12.5]

Application: WB    Species: human    Sample: MRC-5 cell

Figure 4. VGF activates CAFs to support SCLC proliferation and survival in the TME, A, RT-qPCR analysis of mRNA levels of CAF activation markers in MRC-5 cells after coculture with CM from indicated SCLC cells. B, Western blot analysis of αSMA and POSTN protein levels, two classic markers of CAF activation, in MRC-5 cells after coculture with CM from indicated SCLC cells. C–E, RT-qPCR analysis of mRNA levels of CAF activation markers in MRC-5 cells after coculture with CM from SCLC cells with VGF knockdown (C and D) or VGF overexpression (E). F, Western blot analysis of αSMA and POSTN protein levels in MRC-5 cells after coculture with CM from SCLC cells with VGF knockdown or overexpression. G and H, Immunofluorescence (G) and quantitative analysis (H) of αSMA expression and fibrillation in MRC-5 cells after coculture with CM from SCLC cells with VGF knockdown or overexpression. Scale bar, 5 μm. I and J, Wound healing and transwell assays to assess migration and invasion of MRC-5 cells after coculture with CM from SCLC cells with VGF knockdown or overexpression. Scale bar, 1 mm. K, Western blot analysis of TGFBR2, pSMAD2/3, and SMAD2/3 protein levels in MRC-5 cells following VGF overexpression or treatment with rVGF. L, CCK-8 assay measuring cell viability of SCLC cells after coculture with human primary isolated CAFs activated by CM from indicated SCLC cells. M, CCK-8 assay measuring cell viability of SCLC cells after coculture with human primary isolated CAFs activated by CM from SCLC cells with VGF knockdown or overexpression. Differences between groups were assessed using the unpaired t test. *, P < 0.05; **, P < 0.01; ***, P < 0.001. n = 3. Data are presented as the means ± SEM.
2). Sodium Alginate Microgel-Wrapped Primary Human Peritoneal Mesothelial Cells for the Treatment of Peritoneal Fibrosis Caused by Dialysis via the POSTN/NF-κB/CXCL8 Pathway. Frontiers in bioscience (Landmark edition), 2025 (PubMed: 41351397) [IF=3.3]

Application: IF/ICC    Species: human    Sample:

Fig. 3. Increased expression of periostin/nuclear factor kappa-B/C-X-C motif chemokine ligand 8 (POSTN/NF-κB/CXCL8) in long-term PD patients with peritoneal fibrosis. (a) Heatmap showing differential gene expression in PMCs from 1W-PD and 1Y-PD patients. (b) Representative immunofluorescence images of staining for POSTN, NF-κB P65, p-P65, CXCL8, α-smooth muscle actin (α-SMA), collagen I (Col-1), transforming growth factor-β (TGF-β), and FN in PMCs from 1W-PD and 1Y-PD patients. Scale bar = 50 µm. (c–i) mRNA expression levels in the two groups. (j–o) Representative Western blot images and quantitative analysis of POSTN, p-P65/P65, CXCL8, α-SMA, and Col-1 expression in the two groups.
3). Periostin Induces Epithelial-Mesenchymal Transition via p38-MAPK Pathway in Human Renal Tubular Cells by High Glucose. Immunity, inflammation and disease, 2024 (PubMed: 39570100) [IF=3.2]

Application: WB    Species: human    Sample: HK-2 cells

Figure 2 Western blot analysis of periostin protein expression in HK-2 cells stimulated by HG (22 mmol/L) and high concentration of TGF-β1 (10 ng/mL); Image J software was used to collect the gray value of protein bands. *p 
4). POSTN Regulates Fibroblast Proliferation and Migration in Laryngotracheal Stenosis Through the TGF-β/RHOA Pathway. The Laryngoscope, 2024 (PubMed: 38771155) [IF=2.2]

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