cdc45 Antibody | Affinity Biosciences

1/1

cdc45 Antibody - Western blot analysis of extracts from K562, using cdc45 Antibody.

Product:	cdc45 Antibody
Catalog:	DF6779
Description:	Rabbit polyclonal antibody to cdc45
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Dog, Chicken
Mol.Wt.:	64kDa; 66kD(Calculated).
Uniprot:	O75419
RRID:	AB_2838741

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(92%), Bovine(92%), Horse(92%), Sheep(92%), Dog(100%), Chicken(83%)

Clonality:

Polyclonal

Specificity:

cdc45 Antibody detects endogenous levels of total cdc45.

RRID:

AB_2838741
Cite Format: Affinity Biosciences Cat# DF6779, RRID:AB_2838741.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CDC 45; CDC 45L; CDC45 (cell division cycle 45, S.cerevisiae homolog) like; CDC45; CDC45 cell division cycle 45 like; CDC45 like; CDC45 related protein; CDC45_HUMAN; CDC45L2; Cell division control protein 45 homolog; Cell division cycle 45 like 2; Cell division cycle 45 like; PORC PI 1; PORC-PI-1;

Immunogens

Immunogen:

Uniprot:

O75419(CDC45_HUMAN) >>Visit HPA database.

Gene(ID):

CDC45(8318)

Expression:

O75419 CDC45_HUMAN:

Widely expressed, highest levels are found in adult testis and thymus and in fetal liver.

Description:

The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2010]

Sequence:

O75419 CDC45_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MFVSDFRKEFYEVVQSQRVLLFVASDVDALCACKILQALFQCDHVQYTLVPVSGWQELETAFLEHKEQFHYFILINCGANVDLLDILQPDEDTIFFVCDTHRPVNVVNVYNDTQIKLLIKQDDDLEVPAYEDIFRDEEEDEEHSGNDSDGSEPSEKRTRLEEEIVEQTMRRRQRREWEARRRDILFDYEQYEYHGTSSAMVMFELAWMLSKDLNDMLWWAIVGLTDQWVQDKITQMKYVTDVGVLQRHVSRHNHRNEDEENTLSVDCTRISFEYDLRLVLYQHWSLHDSLCNTSYTAARFKLWSVHGQKRLQEFLADMGLPLKQVKQKFQAMDISLKENLREMIEESANKFGMKDMRVQTFSIHFGFKHKFLASDVVFATMSLMESPEKDGSGTDHFIQALDSLSRSNLDKLYHGLELAKKQLRATQQTIASCLCTNLVISQGPFLYCSLMEGTPDVMLFSRPASLSLLSKHLLKSFVCSTKNRRCKLLPLVMAAPLSMEHGTVTVVGIPPETDSSDRKNFFGRAFEKAAESTSSRMLHNHFDLSVIELKAEDRSKFLDALISLLS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Dog

100

Pig

Horse

Bovine

Sheep

Chicken

Xenopus

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75419 As Substrate

O75419

Site	PTM Type	Source
K8	Ubiquitination	Uniprot
S16	Phosphorylation	Uniprot
Y130	Phosphorylation	Uniprot
S144	Phosphorylation	Uniprot
S148	Phosphorylation	Uniprot
S151	Phosphorylation	Uniprot
T168	Phosphorylation	Uniprot
K237	Ubiquitination	Uniprot
T262	Phosphorylation	Uniprot
K301	Ubiquitination	Uniprot
K309	Ubiquitination	Uniprot
K323	Ubiquitination	Uniprot
K326	Ubiquitination	Uniprot
K328	Ubiquitination	Uniprot
K337	Ubiquitination	Uniprot
K350	Ubiquitination	Uniprot
K354	Ubiquitination	Uniprot
T360	Phosphorylation	Uniprot
K411	Ubiquitination	Uniprot
Y413	Phosphorylation	Uniprot
K420	Ubiquitination	Uniprot
K471	Ubiquitination	Uniprot
K475	Ubiquitination	Uniprot
K482	Ubiquitination	Uniprot
T513	Phosphorylation	Uniprot
R524	Methylation	Uniprot
K528	Sumoylation	Uniprot
K528	Ubiquitination	Uniprot
K550	Ubiquitination	Uniprot

Research Backgrounds

O75419_CDC45_HUMAN

Function:

Required for initiation of chromosomal DNA replication.

Subcellular Location:

Cytoplasm. Nucleus.

Tissue Specificity:

Widely expressed, highest levels are found in adult testis and thymus and in fetal liver.

Subunit Structure:

Associated with ORC2. Interacts with HELB.

Family&Domains:

Belongs to the CDC45 family.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)

References

1). ALKBH5-mediated m 6 A modification of CDC6 mRNA impairs gastric cancer tumorigenesis and has prognostic significance. Research Square, 2023

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

cdc45 Antibody - #DF6779