PMS1 Antibody - #DF6906
(3)
| Product: | PMS1 Antibody |
| Catalog: | DF6906 |
| Description: | Rabbit polyclonal antibody to PMS1 |
| Application: | WB IHC |
| Reactivity: | Human, Rat |
| Prediction: | Pig, Dog |
| Mol.Wt.: | 106kDa; 106kD(Calculated). |
| Uniprot: | P54277 |
| RRID: | AB_2838865 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Rat
Prediction:
Pig(82%), Dog(100%)
Clonality:
Polyclonal
Specificity:
PMS1 Antibody detects endogenous levels of total PMS1.
RRID:
AB_2838865
Cite Format: Affinity Biosciences Cat# DF6906, RRID:AB_2838865.
Cite Format: Affinity Biosciences Cat# DF6906, RRID:AB_2838865.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
DNA mismatch repair protein PMS1; FLJ98259; HNPCC3; hPMS1; Human homolog of yeast mutL; Mismatch repair gene PMSL1; pms1; PMS1 postmeiotic segregation increased 1 (S. cerevisiae); PMS1 postmeiotic segregation increased 1; PMS1 protein homolog 1; PMS1_HUMAN; PMSL1; Rhabdomyosarcoma antigen MU RMS 40.10B; Rhabdomyosarcoma antigen MU RMS 40.10E;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Description:
PMS1 belongs to the DNA mismatch repair mutL/hexB family. It is thought to be involved in the repair of DNA mismatches, and it can form heterodimers with MLH1, a known DNA mismatch repair protein. Mutations in PMS1 cause hereditary nonpolyposis colorectal cancer type 3 (HNPCC3) either alone or in combination with mutations in other proteins involved in the HNPCC phenotype, which is also known as Lynch syndrome (1).
Sequence:
- P54277 PMS1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKQLPAATVRLLSSSQIITSVVSVVKELIENSLDAGATSVDVKLENYGFDKIEVRDNGEGIKAVDAPVMAMKYYTSKINSHEDLENLTTYGFRGEALGSICCIAEVLITTRTAADNFSTQYVLDGSGHILSQKPSHLGQGTTVTALRLFKNLPVRKQFYSTAKKCKDEIKKIQDLLMSFGILKPDLRIVFVHNKAVIWQKSRVSDHKMALMSVLGTAVMNNMESFQYHSEESQIYLSGFLPKCDADHSFTSLSTPERSFIFINSRPVHQKDILKLIRHHYNLKCLKESTRLYPVFFLKIDVPTADVDVNLTPDKSQVLLQNKESVLIALENLMTTCYGPLPSTNSYENNKTDVSAADIVLSKTAETDVLFNKVESSGKNYSNVDTSVIPFQNDMHNDESGKNTDDCLNHQISIGDFGYGHCSSEISNIDKNTKNAFQDISMSNVSWENSQTEYSKTCFISSVKHTQSENGNKDHIDESGENEEEAGLENSSEISADEWSRGNILKNSVGENIEPVKILVPEKSLPCKVSNNNYPIPEQMNLNEDSCNKKSNVIDNKSGKVTAYDLLSNRVIKKPMSASALFVQDHRPQFLIENPKTSLEDATLQIEELWKTLSEEEKLKYEEKATKDLERYNSQMKRAIEQESQMSLKDGRKKIKPTSAWNLAQKHKLKTSLSNQPKLDELLQSQIEKRRSQNIKMVQIPFSMKNLKINFKKQNKVDLEEKDEPCLIHNLRFPDAWLMTSKTEVMLLNPYRVEEALLFKRLLENHKLPAEPLEKPIMLTESLFNGSHYLDVLYKMTADDQRYSGSTYLSDPRLTANGFKIKLIPGVSITENYLEIEGMANCLPFYGVADLKEILNAILNRNAKEVYECRPRKVISYLEGEAVRLSRQLPMYLSKEDIQDIIYRMKHQFGNEIKECVHGRPFFHHLTYLPETT
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Dog
100
Pig
82
Bovine
73
Horse
64
Rabbit
64
Sheep
55
Chicken
40
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P54277 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K2 | Ubiquitination | Uniprot | |
| T8 | Phosphorylation | Uniprot | |
| S13 | Phosphorylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| K43 | Ubiquitination | Uniprot | |
| K51 | Ubiquitination | Uniprot | |
| K62 | Ubiquitination | Uniprot | |
| K72 | Ubiquitination | Uniprot | |
| K77 | Ubiquitination | Uniprot | |
| K150 | Ubiquitination | Uniprot | |
| K156 | Ubiquitination | Uniprot | |
| K200 | Ubiquitination | Uniprot | |
| S204 | Phosphorylation | Uniprot | |
| K270 | Ubiquitination | Uniprot | |
| Y280 | Phosphorylation | Uniprot | |
| K283 | Ubiquitination | Uniprot | |
| T311 | Phosphorylation | Uniprot | |
| K314 | Ubiquitination | Uniprot | |
| S315 | Phosphorylation | Uniprot | |
| K362 | Ubiquitination | Uniprot | |
| K372 | Sumoylation | Uniprot | |
| K372 | Ubiquitination | Uniprot | |
| K378 | Ubiquitination | Uniprot | |
| K505 | Methylation | Uniprot | |
| K505 | Sumoylation | Uniprot | |
| K505 | Ubiquitination | Uniprot | |
| S507 | Phosphorylation | Uniprot | |
| K522 | Acetylation | Uniprot | |
| Y563 | Phosphorylation | Uniprot | |
| Y620 | Phosphorylation | Uniprot | |
| K626 | Methylation | Uniprot | |
| S673 | Phosphorylation | Uniprot | |
| K688 | Ubiquitination | Uniprot | |
| K721 | Ubiquitination | Uniprot | |
| T806 | Phosphorylation | Uniprot | |
| Y807 | Phosphorylation | Uniprot | |
| S809 | Phosphorylation | Uniprot | |
| K863 | Acetylation | Uniprot | |
| S875 | Phosphorylation | Uniprot | |
| S885 | Phosphorylation | Uniprot | |
| Y891 | Phosphorylation | Uniprot | |
| S893 | Phosphorylation | Uniprot | |
| K894 | Acetylation | Uniprot | |
| K894 | Ubiquitination | Uniprot | |
| K905 | Acetylation | Uniprot | |
| K913 | Ubiquitination | Uniprot |
Research Backgrounds
Function:
Probably involved in the repair of mismatches in DNA.
Subcellular Location:
Nucleus.
Subunit Structure:
Component of the DNA mismatch repair (MMR) complex composed at least of MSH2, MSH3, MSH6, PMS1 and MLH1. The MutL-beta complex is a heterodimer of PMS1 and MLH1. Interacts with MCM9.
Family&Domains:
Belongs to the DNA mismatch repair MutL/HexB family.
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