Vitamin D Receptor Antibody | Affinity Biosciences

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Vitamin D Receptor Antibody - Western blot analysis of extracts from T-47D, using VDR antibody.

Vitamin D Receptor Antibody - DF6917 at 1/100 staining Rat heart tissue by IHC-P.

Product:	Vitamin D Receptor Antibody
Catalog:	DF6917
Description:	Rabbit polyclonal antibody to Vitamin D Receptor
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Dog, Chicken
Mol.Wt.:	48kDa; 48kD(Calculated).
Uniprot:	P11473
RRID:	AB_2838876

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(83%), Horse(86%), Dog(86%), Chicken(100%)

Clonality:

Polyclonal

Specificity:

Vitamin D Receptor Antibody detects endogenous levels of total Vitamin D Receptor.

RRID:

AB_2838876
Cite Format: Affinity Biosciences Cat# DF6917, RRID:AB_2838876.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

1 25 dihydroxyvitamin D3 receptor; 1; 1,25 dihydroxyvitamin D3 receptor; 1,25-@dihydroxyvitamin D3 receptor; 25-dihydroxyvitamin D3 receptor; Member 1; NR1I1; Nuclear receptor subfamily 1 group I member 1; PPP1R163; Protein phosphatase 1, regulatory subunit 163; VDR; VDR_HUMAN; Vitamin D (1,25- dihydroxyvitamin D3) receptor; Vitamin D hormone receptor; Vitamin D nuclear receptor variant 1; Vitamin D receptor; Vitamin D3 receptor;

Immunogens

Immunogen:

Uniprot:

P11473(VDR_HUMAN) >>Visit HPA database.

Gene(ID):

VDR(7421)

Description:

The vitamin D receptor (VDR), also known as the calcitriol receptor, and also known as NR1I1 (nuclear receptor subfamily 1, group I, member 1), is a member of the nuclear receptor family of transcription factors. Upon activation by vitamin D, the VDR forms a heterodimer with the retinoid-X receptor and binds to hormone response elements on DNA resulting in expression or trans-repression of specific gene products.It is an intracellular hormone receptor that specifically binds 1,25(OH)2D3 and mediates its effects. Downstream targets of this nuclear hormone receptor are principally involved in mineral metabolism though the receptor regulates a variety of other metabolic pathways, such as those involved in the immune response and cancer. Defects in VDR are the cause of rickets vitamin D-dependent type 2A (VDDR2A). A disorder of vitamin D metabolism results in severe rickets, hypocalcemia and secondary hyperparathyroidism. Most patients have total alopecia in addition to rickets. This antibody is a rabbit polyclonal antibody to human VDR.

Sequence:

P11473 VDR_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEAMAASTSLPDPGDFDRNVPRICGVCGDRATGFHFNAMTCEGCKGFFRRSMKRKALFTCPFNGDCRITKDNRRHCQACRLKRCVDIGMMKEFILTDEEVQRKREMILKRKEEEALKDSLRPKLSEEQQRIIAILLDAHHKTYDPTYSDFCQFRPPVRVNDGGGSHPSRPNSRHTPSFSGDSSSSCSDHCITSSDMMDSSSFSNLDLSEEDSDDPSVTLELSQLSMLPHLADLVSYSIQKVIGFAKMIPGFRDLTSEDQIVLLKSSAIEVIMLRSNESFTMDDMSWTCGNQDYKYRVSDVTKAGHSLELIEPLIKFQVGLKKLNLHEEEHVLLMAICIVSPDRPGVQDAALIEAIQDRLSNTLQTYIRCRHPPPGSHLLYAKMIQKLADLRSLNEEHSKQYRCLSFQPECSMKLTPLVLEVFGNEIS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Chicken

100

Horse

Dog

Bovine

Xenopus

Rabbit

Sheep

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P11473 As Substrate

P11473

Site	PTM Type	Enzyme	Source
S51	Phosphorylation	P17252 (PRKCA) , P05771 (PRKCB)	Uniprot
K117	Ubiquitination		Uniprot
S119	Phosphorylation		Uniprot
K123	Ubiquitination		Uniprot
S125	Phosphorylation		Uniprot
S172	Phosphorylation		Uniprot
T175	Phosphorylation		Uniprot
S182	Phosphorylation	P17612 (PRKACA)	Uniprot
S208	Phosphorylation	P68400 (CSNK2A1)	Uniprot
Y293	Phosphorylation		Uniprot
K302	Ubiquitination		Uniprot
Y380	Phosphorylation		Uniprot
K386	Ubiquitination		Uniprot
S392	Phosphorylation		Uniprot
K399	Ubiquitination		Uniprot

Research Backgrounds

P11473_VDR_HUMAN

Function:

Nuclear receptor for calcitriol, the active form of vitamin D3 which mediates the action of this vitamin on cells. Enters the nucleus upon vitamin D3 binding where it forms heterodimers with the retinoid X receptor/RXR. The VDR-RXR heterodimers bind to specific response elements on DNA and activate the transcription of vitamin D3-responsive target genes. Plays a central role in calcium homeostasis (By similarity).

Subcellular Location:

Nucleus. Cytoplasm.
Note: Localizes mainly to the nucleus (PubMed:28698609, PubMed:12145331). Localization to the nucleus is enhanced by vitamin D3.

Subunit Structure:

Homodimer in the absence of bound vitamin D3. Heterodimer with RXRA after vitamin D3 binding. Interacts with MED1, NCOA1, NCOA2, NCOA3 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Interacts with the corepressor NCOR1. Interacts with SNW1. Interacts with IRX4, the interaction does not affect its transactivation activity. Interacts with CRY1 (By similarity). Interacts with CRY2 in a ligand-dependent manner (By similarity).

Family&Domains:

Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.

The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.

Belongs to the nuclear hormone receptor family. NR1 subfamily.

Research Fields

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Organismal Systems > Excretory system > Endocrine and other factor-regulated calcium reabsorption.

· Organismal Systems > Digestive system > Mineral absorption.

References

1). Vitexin Regulates Angiogenesis and Osteogenesis in Ovariectomy-Induced Osteoporosis of Rats via the VDR/PI3K/AKT/eNOS Signaling Pathway. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2022 (PubMed: 36538589) [IF=5.7]

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Vitamin D Receptor Antibody - #DF6917