P2RY6 Antibody - #DF6956

Figure 1. Macrophage P2Y6R expression is increased in psoriatic lesional skin and involved in Th1 cells mediated psoriasis processes. (A) Expression of purinergic receptors in human and mice psoriasis skin. (B) Linear regression analysis indicated a positive correlation between P2ry6 mRNA expression and PASI score in GSE117468. (C) Flow diagram. (D) Skin thickness was measured from Day 0 to Day 6 (n = 12). (E) Represent images for the psoriatic appearance on IMQ or Vaseline-treated WT mice and P2Y6R−/− mice skin. (F) The representative image of spleen from the mice. (G) The spleen index on Day 6 (n = 12). (H) H&E staining of IMQ-treated WT mice and P2Y6R−/− mice skin (n = 3). (I) Ki67 staining of skin tissues from WT or P2Y6R−/− mice treated with Vaseline or IMQ (n = 3). (J) Immune infiltration correlation coefficient of lymphoid cells and myeloid cells of P2ry6 in GSE181318 and GSE14905. (K) scRNA-seq data of GSE151177 were grouped into 8 clusters with UMAP. (L) Expression of P2Y6R in different cells form scRNA-seq data. (M) Represent immunofluorescence images of Macrophages CD86 (red) and P2Y6R (green) co-localization in mice skin treated with Vaseline or IMQ (n = 3). Scale bar = 50 μm. (N) CellChat analysis indicated the inferred interaction among macrophages in psoriasis patient. (O–P) Th1 cells (CD4+ IFNγ+) proportion was decreased in IMQ-induced P2Y6R−/− mice skin and spleen (n = 3). Data are expressed as mean ± SEM. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.

Figure 4. IMQ-induced psoriasis mice is reduced in Lyz2creP2Y6Rfl/fl mice. (A) Experimental scheme. (B) Representative skin pictures of Lyz2creP2Y6Rfl/fl and P2Y6Rfl/fl mice. (C) Pictures of spleen and (D) spleen index from all groups (n = 12). (E, F) H&E staining (n = 3) and skin thickness, erythema, and scaling PASI score (n = 12) of IMQ or Vaseline-treated Lyz2cre P2Y6Rfl/fl and P2Y6Rfl/fl mice skin. (G) Ki67 staining of IMQ-treated skin tissues from P2Y6Rfl/fl or Lyz2creP2Y6Rfl/fl mice treated with Saline or IL-27 (n = 3). (H) Serum and skin concentrations of IL-27 and IFNγ detected by ELISA assay (n = 4). (I, J) Flow analysis of Th1 cells from skin and spleens of Lyz2creP2Y6Rfl/fl and P2Y6Rfl/fl mice treated with IMQ or Vaseline (n = 3). (K–M) Quantitative analysis of protein expression in mice skin. Normal protein levels were normalized to β-actin, and the values for the phosphorylated forms of proteins were normalized to phosphorylation-independent levels of the same protein. (n = 4). (N) The mRNA expression levels of Th1-related cytokine and IL-27 p28 and Ebi3 in skin tissues (n = 3). All mRNA levels were normalized to GAPDH mRNA. Data are expressed as mean ± SEM. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.