TMPO Antibody - #DF6994

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Product: TMPO Antibody
Catalog: DF6994
Description: Rabbit polyclonal antibody to TMPO
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 75kDa; 51kD(Calculated).
Uniprot: P42167
RRID: AB_2838950

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
TMPO Antibody detects endogenous levels of total TMPO.
RRID:
AB_2838950
Cite Format: Affinity Biosciences Cat# DF6994, RRID:AB_2838950.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Lamina associated polypeptide 2; LAP 2; LEM domain containing 4; LEMD4; Thymopoietin; Thymopoietin isoform alpha; Thymopoietin related peptide isoform alpha; Thymopoietin related peptide isoforms beta/gamma; Thymopoietin, isoforms beta/gamma; TMPO; TP alpha; TP; TP beta/gamma; TPRP isoform alpha; TPRP isoforms beta/gamma;

Immunogens

Immunogen:

A synthesized peptide derived from human TMPO, corresponding to a region within the internal amino acids.

Uniprot:

P42167(LAP2B_HUMAN) >>Visit HPA database.

Gene(ID):
TMPO(7112)
Expression:
P42167 LAP2B_HUMAN:

Expressed in many tissues. Most abundant in adult thymus and fetal liver.

Description:
The protein encoded by this gene resides in the nucleus and may play a role in the assembly of the nuclear lamina, and thus help maintain the structural organization of the nuclear envelope. It may function as a receptor for the attachment of lamin filaments to the inner nuclear membrane. Mutations in this gene are associated with dilated cardiomyopathy. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
Sequence:
MPEFLEDPSVLTKDKLKSELVANNVTLPAGEQRKDVYVQLYLQHLTARNRPPLPAGTNSKGPPDFSSDEEREPTPVLGSGAAAAGRSRAAVGRKATKKTDKPRQEDKDDLDVTELTNEDLLDQLVKYGVNPGPIVGTTRKLYEKKLLKLREQGTESRSSTPLPTISSSAENTRQNGSNDSDRYSDNEEDSKIELKLEKREPLKGRAKTPVTLKQRRVEHNQSYSQAGITETEWTSGSSKGGPLQALTRESTRGSRRTPRKRVETSEHFRIDGPVISESTPIAETIMASSNESLVVNRVTGNFKHASPILPITEFSDIPRRAPKKPLTRAEVGEKTEERRVERDILKEMFPYEASTPTGISASCRRPIKGAAGRPLELSDFRMEESFSSKYVPKYVPLADVKSEKTKKGRSIPVWIKILLFVVVAVFLFLVYQAMETNQVNPFSNFLHVDPRKSN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P42167 As Substrate

Site PTM Type Enzyme
S9 Phosphorylation
T12 Phosphorylation
K13 Sumoylation
K13 Ubiquitination
K17 Sumoylation
K17 Ubiquitination
S18 Phosphorylation
T26 Phosphorylation
R50 Methylation
T57 Phosphorylation
S59 Phosphorylation
K60 Acetylation
K60 Ubiquitination
S66 Phosphorylation
S67 Phosphorylation
T74 Phosphorylation
S79 Phosphorylation
R86 Methylation
S87 Phosphorylation
T96 Phosphorylation
T99 Phosphorylation
K107 Ubiquitination
T113 Phosphorylation
T116 Phosphorylation
K126 Ubiquitination
Y127 Phosphorylation
T137 Phosphorylation
T154 Phosphorylation
S156 Phosphorylation
S158 Phosphorylation
S159 Phosphorylation
T160 Phosphorylation
T164 Phosphorylation
S166 Phosphorylation
S167 Phosphorylation
S168 Phosphorylation
T172 Phosphorylation
S177 Phosphorylation
S180 Phosphorylation
Y183 Phosphorylation
S184 Phosphorylation
S190 Phosphorylation
K198 Ubiquitination
K207 Acetylation
K207 Methylation
K207 Ubiquitination
T208 Phosphorylation
T211 Phosphorylation
K213 Ubiquitination
S222 Phosphorylation
Y223 Phosphorylation
S224 Phosphorylation
S235 Phosphorylation
K239 Ubiquitination
T247 Phosphorylation
S250 Phosphorylation
T251 Phosphorylation
S254 Phosphorylation
T257 Phosphorylation
T264 Phosphorylation
S265 Phosphorylation
S276 Phosphorylation
T279 Phosphorylation
T284 Phosphorylation
S288 Phosphorylation
S289 Phosphorylation
S292 Phosphorylation
T299 Phosphorylation
K303 Methylation
K303 Ubiquitination
S306 Phosphorylation
T312 Phosphorylation
S315 Phosphorylation
K323 Ubiquitination
K324 Ubiquitination
K334 Acetylation
K334 Ubiquitination
T335 Phosphorylation
K346 Ubiquitination
Y351 Phosphorylation
S354 Phosphorylation
T355 Phosphorylation
T357 Phosphorylation
S360 Phosphorylation
S362 Phosphorylation
K368 Ubiquitination
S378 Phosphorylation
S385 Phosphorylation
S387 Phosphorylation
S388 Phosphorylation
K389 Ubiquitination
Y390 Phosphorylation
K393 Ubiquitination
Y394 Phosphorylation
K401 Ubiquitination
S402 Phosphorylation
K404 Ubiquitination

Research Backgrounds

Function:

May help direct the assembly of the nuclear lamina and thereby help maintain the structural organization of the nuclear envelope. Possible receptor for attachment of lamin filaments to the inner nuclear membrane. May be involved in the control of initiation of DNA replication through its interaction with NAKAP95.

Thymopoietin (TP) and Thymopentin (TP5) may play a role in T-cell development and function. TP5 is an immunomodulating pentapeptide.

PTMs:

Mitosis-specific phosphorylation specifically abolishes its binding to lamin B and chromosomes.

Citrullinated by PADI4.

Subcellular Location:

Nucleus inner membrane>Single-pass type II membrane protein.
Note: Tightly associated with the nuclear lamina.

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in many tissues. Most abundant in adult thymus and fetal liver.

Subunit Structure:

Interacts with LMNB1, LMNB2, BANF1, AKAP8L, GMCL and chromosomes (By similarity). Isoform Zeta interacts with BANF1/BAF and may sequester it in the cytoplasm.

Family&Domains:

Has two structurally independent, non-interacting domains: LEM-like (also called LAP2-N or LEM-D) and LEM (also called LAP2-C or LEM-B). LEM-like binds DNA while LEM interacts with BANF1.

Belongs to the LEM family.

Restrictive clause

 

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