DLK1 Antibody | Affinity Biosciences

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DLK1 Antibody - Western blot analysis of extracts from mouse brain, using DLK1 Antibody.

Product:	DLK1 Antibody
Catalog:	DF7062
Description:	Rabbit polyclonal antibody to DLK1
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Rabbit
Mol.Wt.:	41kDa; 41kD(Calculated).
Uniprot:	P80370
RRID:	AB_2839018

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Rabbit(100%)

Clonality:

Polyclonal

Specificity:

DLK1 Antibody detects endogenous levels of total DLK1.

RRID:

AB_2839018
Cite Format: Affinity Biosciences Cat# DF7062, RRID:AB_2839018.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Adipocyte differentiation inhibitor protein; Brevideltin; Delta like 1 homolog; Delta like homolog; Delta like protein; Delta1; DLK 1; DLK; DLK-1; DLK1; DLK1_HUMAN; FA 1; FA1; Fetal antigen 1; pG2; Preadipocyte factor 1; Pref 1; Pref; Pref1; Protein delta homolog 1; Secredeltin; ZOG;

Immunogens

Immunogen:

Uniprot:

P80370(DLK1_HUMAN) >>Visit HPA database.

Gene(ID):

DLK1(8788)

Expression:

P80370 DLK1_HUMAN:

Found within the stromal cells in close contact to the vascular structure of placental villi, yolk sac, fetal liver, adrenal cortex and pancreas and in the beta cells of the islets of Langerhans in the adult pancreas. Found also in some forms of neuroendocrine lung tumor tissue.

Description:

DLK1 (delta-like-1), also known as fetal antigen 1 (FA1) and preadipocyte factor 1 (pref-1), is a member of the epidermal growth factor (EGF)-like family of proteins, containing six tandem EGF-like repeats (1,2). DLK1 is a paternally expressed, imprinted gene that plays an important role in normal development and in the maintenence of homeostasis of adipose tissue mass (3). DLK1 deficient mice display growth retardation, obesity, skeletal malformation, and increased serum lipid metabolites (4). It has been reported that the ectodomain of DLK1 is shredded from the cell surface and inhibits adipocyte differentiation ( 5-7).

Sequence:

P80370 DLK1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MTATEALLRVLLLLLAFGHSTYGAECFPACNPQNGFCEDDNVCRCQPGWQGPLCDQCVTSPGCLHGLCGEPGQCICTDGWDGELCDRDVRACSSAPCANNRTCVSLDDGLYECSCAPGYSGKDCQKKDGPCVINGSPCQHGGTCVDDEGRASHASCLCPPGFSGNFCEIVANSCTPNPCENDGVCTDIGGDFRCRCPAGFIDKTCSRPVTNCASSPCQNGGTCLQHTQVSYECLCKPEFTGLTCVKKRALSPQQVTRLPSGYGLAYRLTPGVHELPVQQPEHRILKVSMKELNKKTPLLTEGQAICFTILGVLTSLVVLGTVGIVFLNKCETWVSNLRYNHMLRKKKNLLLQYNSGEDLAVNIIFPEKIDMTTFSKEAGDEEI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Pig

Horse

Bovine

Dog

Chicken

Sheep

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P80370 As Substrate

P80370

Site	PTM Type	Source
S94	O-Glycosylation	Uniprot
N100	N-Glycosylation	Uniprot
T143	O-Glycosylation	Uniprot
S163	O-Glycosylation	Uniprot
N165	N-Glycosylation	Uniprot
N172	N-Glycosylation	Uniprot
S214	O-Glycosylation	Uniprot
T222	O-Glycosylation	Uniprot
S251	O-Glycosylation	Uniprot
T256	O-Glycosylation	Uniprot
S260	O-Glycosylation	Uniprot
T269	O-Glycosylation	Uniprot
Y353	Phosphorylation	Uniprot
S355	Phosphorylation	Uniprot
K376	Ubiquitination	Uniprot

Research Backgrounds

P80370_DLK1_HUMAN

Function:

May have a role in neuroendocrine differentiation.

PTMs:

N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.

Subcellular Location:

Membrane>Single-pass type I membrane protein. Cytoplasm.

Tissue Specificity:

Subunit Structure:

Monomer. Interacts with SH3RF2 (By similarity).

Restrictive clause

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For Research Use Only.
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DLK1 Antibody - #DF7062