HSF2 Antibody | Affinity Biosciences

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HSF2 Antibody - Western blot analysis of extracts from HepG2 cells, using HSF2 Antibody.

Product:	HSF2 Antibody
Catalog:	DF7072
Description:	Rabbit polyclonal antibody to HSF2
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
Mol.Wt.:	60kDa, 50 kDa; 60kD(Calculated).
Uniprot:	Q03933
RRID:	AB_2839028

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%), Xenopus(83%)

Clonality:

Polyclonal

Specificity:

HSF2 Antibody detects endogenous levels of total HSF2.

RRID:

AB_2839028
Cite Format: Affinity Biosciences Cat# DF7072, RRID:AB_2839028.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Heat shock factor protein 2; Heat shock transcription factor 2; HSF 2; HSF2; HSF2_HUMAN; HSTF 2;

Immunogens

Immunogen:

Uniprot:

Q03933(HSF2_HUMAN) >>Visit HPA database.

Gene(ID):

HSF2(3298)

Description:

The protein encoded by this gene belongs to the HSF family of transcription factors that bind specifically to the heat-shock promoter element and activate transcription. Heat shock transcription factors activate heat-shock response genes under conditions of heat or other stresses. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Sequence:

Q03933 HSF2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKQSSNVPAFLSKLWTLVEETHTNEFITWSQNGQSFLVLDEQRFAKEILPKYFKHNNMASFVRQLNMYGFRKVVHIDSGIVKQERDGPVEFQHPYFKQGQDDLLENIKRKVSSSKPEENKIRQEDLTKIISSAQKVQIKQETIESRLSELKSENESLWKEVSELRAKHAQQQQVIRKIVQFIVTLVQNNQLVSLKRKRPLLLNTNGAQKKNLFQHIVKEPTDNHHHKVPHSRTEGLKPRERISDDIIIYDVTDDNADEENIPVIPETNEDVISDPSNCSQYPDIVIVEDDNEDEYAPVIQSGEQNEPARESLSSGSDGSSPLMSSAVQLNGSSSLTSEDPVTMMDSILNDNINLLGKVELLDYLDSIDCSLEDFQAMLSGRQFSIDPDLLVDLFTSSVQMNPTDYINNTKSENKGLETTKNNVVQPVSEEGRKSKSKPDKQLIQYTAFPLLAFLDGNPASSVEQASTTASSEVLSSVDKPIEVDELLDSSLDPEPTQSKLVRLEPLTEAEASEATLFYLCELAPAPLDSDMPLLDS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Chicken

100

Rabbit

100

Xenopus

Zebrafish

Dog

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q03933 As Substrate

Q03933

Site	PTM Type	Source
K51	Ubiquitination	Uniprot
K54	Ubiquitination	Uniprot
K82	Sumoylation	Uniprot
K82	Ubiquitination	Uniprot
K97	Ubiquitination	Uniprot
K108	Ubiquitination	Uniprot
K110	Ubiquitination	Uniprot
K128	Ubiquitination	Uniprot
K135	Sumoylation	Uniprot
K135	Ubiquitination	Uniprot
K139	Sumoylation	Uniprot
K139	Ubiquitination	Uniprot
T142	Phosphorylation	Uniprot
K151	Sumoylation	Uniprot
K151	Ubiquitination	Uniprot
K159	Ubiquitination	Uniprot
K167	Ubiquitination	Uniprot
K197	Ubiquitination	Uniprot
K210	Ubiquitination	Uniprot
K420	Ubiquitination	Uniprot

Research Backgrounds

Q03933_HSF2_HUMAN

Function:

DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Cytoplasmic during normal growth and moves to the nucleus upon activation.

Subunit Structure:

DNA-binding homotrimer in stressed or heat shocked cells, otherwise found as a homodimer.

Family&Domains:

Belongs to the HSF family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

HSF2 Antibody - #DF7072