Mitofilin Antibody - #DF7074

(2)  
Product: Mitofilin Antibody
Catalog: DF7074
Description: Rabbit polyclonal antibody to Mitofilin
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 84kDa, 100 kDa; 84kD(Calculated).
Uniprot: Q16891
RRID: AB_2839030

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:100, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Bovine(83%), Horse(92%), Sheep(83%), Rabbit(83%), Dog(92%)
Clonality:
Polyclonal
Specificity:
Mitofilin Antibody detects endogenous levels of total Mitofilin.
RRID:
AB_2839030
Cite Format: Affinity Biosciences Cat# DF7074, RRID:AB_2839030.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cell proliferation inducing protein 52; Cell proliferation-inducing gene 4/52 protein; Heart muscle protein; HMP; IMMT; IMMT_HUMAN; Inner membrane protein mitochondrial; Inner mitochrondial membrane; MICOS complex subunit MIC60; MINOS2; Mitochondrial inner membrane organizing system 2; Mitochondrial inner membrane protein; Mitofilin; Motor protein; p87; p87/89; p89; pig4; PIG52; Proliferation-inducing gene 4;

Immunogens

Immunogen:
Uniprot:

Q16891(MIC60_HUMAN) >>Visit HPA database.

Gene(ID):
IMMT(10989)
Description:
Mitochondrial inner membrane protein is a protein that in humans is encoded by the IMMT gene.IMMT has been shown to interact with BAT2.The mitochondrial inner membrane (IM) serves as the site for ATP production by hosting the oxidative phosphorylation complex machinery most notably on the crista membranes. Disruption of the crista structure has been implicated in a variety of cardiovascular and neurodegenerative diseases.
Sequence:
MLRACQLSGVTAAAQSCLCGKFVLRPLRPCRRYSTSGSSGLTTGKIAGAGLLFVGGGIGGTILYAKWDSHFRESVEKTIPYSDKLFEMVLGPAAYNVPLPKKSIQSGPLKISSVSEVMKESKQPASQLQKQKGDTPASATAPTEAAQIISAAGDTLSVPAPAVQPEESLKTDHPEIGEGKPTPALSEEASSSSIRERPPEEVAARLAQQEKQEQVKIESLAKSLEDALRQTASVTLQAIAAQNAAVQAVNAHSNILKAAMDNSEIAGEKKSAQWRTVEGALKERRKAVDEAADALLKAKEELEKMKSVIENAKKKEVAGAKPHITAAEGKLHNMIVDLDNVVKKVQAAQSEAKVVSQYHELVVQARDDFKRELDSITPEVLPGWKGMSVSDLADKLSTDDLNSLIAHAHRRIDQLNRELAEQKATEKQHITLALEKQKLEEKRAFDSAVAKALEHHRSEIQAEQDRKIEEVRDAMENEMRTQLRRQAAAHTDHLRDVLRVQEQELKSEFEQNLSEKLSEQELQFRRLSQEQVDNFTLDINTAYARLRGIEQAVQSHAVAEEEARKAHQLWLSVEALKYSMKTSSAETPTIPLGSAVEAIKANCSDNEFTQALTAAIPPESLTRGVYSEETLRARFYAVQKLARRVAMIDETRNSLYQYFLSYLQSLLLFPPQQLKPPPELCPEDINTFKLLSYASYCIEHGDLELAAKFVNQLKGESRRVAQDWLKEARMTLETKQIVEILTAYASAVGIGTTQVQPE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
92
Horse
92
Dog
92
Bovine
83
Sheep
83
Rabbit
83
Xenopus
75
Chicken
75
Zebrafish
67
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q16891 As Substrate

Site PTM Type Enzyme
S34 Phosphorylation
S69 Phosphorylation
S74 Phosphorylation
K77 Ubiquitination
T78 Phosphorylation
S82 Phosphorylation
Y95 Phosphorylation
K102 Ubiquitination
S103 Phosphorylation
S106 Phosphorylation
K110 Ubiquitination
S112 Phosphorylation
S113 Phosphorylation
S115 Phosphorylation
S121 Phosphorylation
K122 Ubiquitination
S126 Phosphorylation
K130 Ubiquitination
K180 Ubiquitination
S186 Phosphorylation
S190 Phosphorylation
K211 Acetylation
K211 Ubiquitination
K216 Acetylation
S219 Phosphorylation
K222 Acetylation
S223 Phosphorylation
S263 Phosphorylation
K282 Acetylation
K282 Ubiquitination
K306 Acetylation
S307 Phosphorylation
S356 Phosphorylation
Y358 Phosphorylation
K385 Acetylation
K385 Ubiquitination
S388 Phosphorylation
S390 Phosphorylation
K395 Ubiquitination
K427 Ubiquitination
T431 Phosphorylation
K436 Ubiquitination
S447 Phosphorylation
K451 Acetylation
K451 Ubiquitination
S458 Phosphorylation
R495 Methylation
K506 Acetylation
K506 Ubiquitination
S507 Phosphorylation
S514 Phosphorylation
K516 Ubiquitination
S528 Phosphorylation
S555 Phosphorylation
S572 Phosphorylation
Y578 Phosphorylation
S579 Phosphorylation
K581 Ubiquitination
S583 Phosphorylation
S584 Phosphorylation
T587 Phosphorylation
T589 Phosphorylation
K600 Ubiquitination
Y626 Phosphorylation
T630 Phosphorylation
Y636 Phosphorylation
K640 Ubiquitination
T651 Phosphorylation
Y693 Phosphorylation
S695 Phosphorylation
Y696 Phosphorylation
K714 Ubiquitination
K726 Ubiquitination
T731 Phosphorylation
T734 Phosphorylation

Research Backgrounds

Function:

Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Plays an important role in the maintenance of the MICOS complex stability and the mitochondrial cristae morphology.

Subcellular Location:

Mitochondrion inner membrane>Single-pass membrane protein. Mitochondrion.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the mitochondrial contact site and cristae organizing system (MICOS) complex, composed of at least MICOS10/MIC10, CHCHD3/MIC19, CHCHD6/MIC25, APOOL/MIC27, IMMT/MIC60, APOO/MIC23/MIC26 and MICOS13/MIC13. This complex was also known under the names MINOS or MitOS complex. The MICOS complex associates with mitochondrial outer membrane proteins SAMM50, MTX1 and MTX2 (together described as components of the mitochondrial outer membrane sorting assembly machinery (SAM) complex) and DNAJC11, mitochondrial inner membrane protein TMEM11 and with HSPA9. The MICOS and SAM complexes together with DNAJC11 are part of a large protein complex spanning both membranes termed the mitochondrial intermembrane space bridging (MIB) complex. Interacts with HSPA1A/HSPA1B and OPA1, preferentially with the soluble OPA1 form (By similarity). Interacts with MICOS13/MIC13, MICOS10/MIC10, CHCHD3/MIC19, CHCHD6/MIC25, SAMM50 and TMEM11. Interacts with APOO/MIC23/MIC26 and APOOL/MIC27.

Family&Domains:

Belongs to the MICOS complex subunit Mic60 family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.