USO1 Antibody - #DF7086

(3)  
Product: USO1 Antibody
Catalog: DF7086
Description: Rabbit polyclonal antibody to USO1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 108kDa; 108kD(Calculated).
Uniprot: O60763
RRID: AB_2839042

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
USO1 Antibody detects endogenous levels of total USO1.
RRID:
AB_2839042
Cite Format: Affinity Biosciences Cat# DF7086, RRID:AB_2839042.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

General vesicular transport factor; General vesicular transport factor p115; P115; Protein USO1 homolog; TAP; Transcytosis associated protein; Transcytosis-associated protein; Uso1; USO1_HUMAN; VDP; Vesicle docking protein; Vesicle-docking protein;

Immunogens

Immunogen:
Uniprot:

O60763(USO1_HUMAN) >>Visit HPA database.

Gene(ID):
USO1(8615)
Description:
The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Transport from the ER to the cis/medial Golgi compartments requires the action of this gene product, GM130 and giantin in a sequential manner.
Sequence:
MNFLRGVMGGQSAGPQHTEAETIQKLCDRVASSTLLDDRRNAVRALKSLSKKYRLEVGIQAMEHLIHVLQTDRSDSEIIGYALDTLYNIISNEEEEEVEENSTRQSEDLGSQFTEIFIKQQENVTLLLSLLEEFDFHVRWPGVKLLTSLLKQLGPQVQQIILVSPMGVSRLMDLLADSREVIRNDGVLLLQALTRSNGAIQKIVAFENAFERLLDIISEEGNSDGGIVVEDCLILLQNLLKNNNSNQNFFKEGSYIQRMKPWFEVGDENSGWSAQKVTNLHLMLQLVRVLVSPTNPPGATSSCQKAMFQCGLLQQLCTILMATGVPADILTETINTVSEVIRGCQVNQDYFASVNAPSNPPRPAIVVLLMSMVNERQPFVLRCAVLYCFQCFLYKNQKGQGEIVSTLLPSTIDATGNSVSAGQLLCGGLFSTDSLSNWCAAVALAHALQENATQKEQLLRVQLATSIGNPPVSLLQQCTNILSQGSKIQTRVGLLMLLCTWLSNCPIAVTHFLHNSANVPFLTGQIAENLGEEEQLVQGLCALLLGISIYFNDNSLESYMKEKLKQLIEKRIGKENFIEKLGFISKHELYSRASQKPQPNFPSPEYMIFDHEFTKLVKELEGVITKAIYKSSEEDKKEEEVKKTLEQHDNIVTHYKNMIREQDLQLEELRQQVSTLKCQNEQLQTAVTQQVSQIQQHKDQYNLLKIQLGKDNQHQGSYSEGAQMNGIQPEEIGRLREEIEELKRNQELLQSQLTEKDSMIENMKSSQTSGTNEQSSAIVSARDSEQVAELKQELATLKSQLNSQSVEITKLQTEKQELLQKTEAFAKSVEVQGETETIIATKTTDVEGRLSALLQETKELKNEIKALSEERTAIKEQLDSSNSTIAILQTEKDKLELEITDSKKEQDDLLVLLADQDQKILSLKNKLKDLGHPVEEEDELESGDQEDEDDESEDPGKDLDHI

PTMs - O60763 As Substrate

Site PTM Type Enzyme
R5 Methylation
S12 Phosphorylation
K25 Ubiquitination
R29 Methylation
S32 Phosphorylation
R39 Methylation
K47 Ubiquitination
S48 Phosphorylation
S50 Phosphorylation
S111 Phosphorylation
S164 Phosphorylation
S169 Phosphorylation
K202 Acetylation
K202 Ubiquitination
K251 Methylation
T465 Phosphorylation
S483 Phosphorylation
S486 Phosphorylation
K580 Ubiquitination
S585 Phosphorylation
S591 Phosphorylation
K596 Ubiquitination
K618 Ubiquitination
K626 Ubiquitination
K643 Ubiquitination
Y655 Phosphorylation
R670 Methylation
K698 Ubiquitination
K705 Ubiquitination
S717 Phosphorylation
Y718 Phosphorylation
K743 Ubiquitination
S751 Phosphorylation
S758 Phosphorylation
S780 Phosphorylation
K791 Ubiquitination
K798 Ubiquitination
K815 Acetylation
S828 Phosphorylation
S851 Phosphorylation
T857 Phosphorylation
K904 Ubiquitination
K919 Ubiquitination
S942 Phosphorylation
S952 Phosphorylation

Research Backgrounds

Function:

General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity.

PTMs:

Phosphorylated in a cell cycle-specific manner; phosphorylated in interphase but not in mitotic cells. Dephosphorylated protein associates with the Golgi membrane; phosphorylation promotes dissociation.

Subcellular Location:

Cytoplasm>Cytosol. Golgi apparatus membrane>Peripheral membrane protein.
Note: Recycles between the cytosol and the Golgi apparatus during interphase. During interphase, the phosphorylated form is found exclusively in cytosol; the unphosphorylated form is associated with Golgi apparatus membranes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer. Dimerizes by parallel association of the tails, resulting in an elongated structure with two globular head domains side by side, and a long rod-like tail structure (Probable). Interacts with MIF.

Family&Domains:

Composed of a globular head, an elongated tail (coiled-coil) and a highly acidic C-terminal domain.

Belongs to the VDP/USO1/EDE1 family.

Restrictive clause

 

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