CX3CR1 Antibody - #DF7096

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Product: CX3CR1 Antibody
Catalog: DF7096
Description: Rabbit polyclonal antibody to CX3CR1
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 44kDa; 40kD(Calculated).
Uniprot: P49238
RRID: AB_2839051

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
CX3CR1 Antibody detects endogenous levels of total CX3CR1.
RRID:
AB_2839051
Cite Format: Affinity Biosciences Cat# DF7096, RRID:AB_2839051.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Beta chemokine receptor-like 1; C X3 C CKR 1; C-X3-C CKR-1; CCRL1; Chemokine C X3 C motif receptor 1; CMK BRL 1; CMK-BRL-1; CMK-BRL1; CMKBLR1; CMKDR1; CX3C chemokine receptor 1; CX3C CKR1; CX3C1_HUMAN; CX3CR1; Fractalkine receptor; G-protein coupled receptor 13; GPR13; GPRV28; V28;

Immunogens

Immunogen:
Uniprot:

P49238(CX3C1_HUMAN) >>Visit HPA database.

Gene(ID):
CX3CR1(1524)
Expression:
P49238 CX3C1_HUMAN:

Expressed in lymphoid and neural tissues.

Description:
Fractalkine is a transmembrane protein and chemokine involved in the adhesion and migration of leukocytes. The protein encoded by this gene is a receptor for fractalkine. The encoded protein also is a coreceptor for HIV-1, and some variations in this gene lead to increased susceptibility to HIV-1 infection and rapid progression to AIDS. Four transcript variants encoding two different isoforms have been found for this gene.
Sequence:
MDQFPESVTENFEYDDLAEACYIGDIVVFGTVFLSIFYSVIFAIGLVGNLLVVFALTNSKKPKSVTDIYLLNLALSDLLFVATLPFWTHYLINEKGLHNAMCKFTTAFFFIGFFGSIFFITVISIDRYLAIVLAANSMNNRTVQHGVTISLGVWAAAILVAAPQFMFTKQKENECLGDYPEVLQEIWPVLRNVETNFLGFLLPLLIMSYCYFRIIQTLFSCKNHKKAKAIKLILLVVIVFFLFWTPYNVMIFLETLKLYDFFPSCDMRKDLRLALSVTETVAFSHCCLNPLIYAFAGEKFRRYLYHLYGKCLAVLCGRSVHVDFSSSESQRSRHGSVLSSNFTYHTSDGDALLLL

PTMs - P49238 As Substrate

Site PTM Type Enzyme
Y128 Phosphorylation
S137 Phosphorylation
Y303 Phosphorylation
S329 Phosphorylation

Research Backgrounds

Function:

Receptor for the CX3C chemokine fractalkine (CX3CL1); binds to CX3CL1 and mediates both its adhesive and migratory functions. Acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro). Isoform 2 and isoform 3 seem to be more potent HIV-1 coreceptors than isoform 1.

PTMs:

This protein is not N-glycosylated which is unusual for G-protein-coupled receptors.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in lymphoid and neural tissues.

Subunit Structure:

Found in a ternary complex with CX3CL1 and ITGAV:ITGB3 or ITGA4:ITGB1.

(Microbial infection) Interacts with human respiratory syncytial virus (HRSV) protein G; this interaction modulates host immune response.

(Microbial infection) Interacts with HIV-1 envelope polyprotein gp160.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

References

1). Targeting hepatocyte MagL regulates the progression of colorectal liver metastases during liver ischemia-reperfusion. Research Square, 2022

Application: IF/ICC    Species: Mouse    Sample:

Figure 4. Hepatocyte MagL promotes inflammation resolution after LIR by affecting macrophage M2 polarization The mice in the siMagL group and the siCtrl group were subjected to partial warm ischemia for 90 minutes, and the ischemic lobes of the mice were harvested after 3 days of reperfusion. A NPCs were sorted, and the expressions of Ly6C, CD11b, CX3CR1 and their corresponding quantification were analyzed by flow cytometry. B Representative immunofluorescence images of CD206 (red) and Mertk (green) in ischemic lobes of the liver, Scale bar, 100mm. C Representative immunofluorescence images of Arg1 (red), CX3CR1 (green) and TGF-β (pink) in the ischemic lobe of the liver, Scale bar, 100mm. D Detection of M2 polarization-related markers and cytokine gene expression in liver ischemic lobes by qPCR. E The protein levels of M2 polarization-related markers in ischemic lobes of liver were detected by Western Blot. Data are presented as mean ± SD. ( *, P < 0.05; * *, P < 0.01). The data represent three separate experiments.

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