GJB3 Antibody - #DF7116

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Product: GJB3 Antibody
Catalog: DF7116
Description: Rabbit polyclonal antibody to GJB3
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Dog
Mol.Wt.: 31kDa; 31kD(Calculated).
Uniprot: O75712
RRID: AB_2839070

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:100
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Dog(90%)
Clonality:
Polyclonal
Specificity:
GJB3 Antibody detects endogenous levels of total GJB3.
RRID:
AB_2839070
Cite Format: Affinity Biosciences Cat# DF7116, RRID:AB_2839070.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Connexin 31; Connexin-31; Connexin31; CX 31; Cx31; CXB3_HUMAN; DFNA 2; DFNA2; DFNA2B; EKV; FLJ22486; Gap junction beta 3 protein; Gap junction beta-3 protein; Gap junction protein beta 3 31kDa; Gap junction protein beta 3; GJB 3; Gjb3; MGC102938;

Immunogens

Immunogen:
Uniprot:

O75712(CXB3_HUMAN) >>Visit HPA database.

Gene(ID):
GJB3(2707)
Description:
This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene can cause non-syndromic deafness or erythrokeratodermia variabilis, a skin disorder. Alternative splicing results in multiple transcript variants encoding the same protein.
Sequence:
MDWKTLQALLSGVNKYSTAFGRIWLSVVFVFRVLVYVVAAERVWGDEQKDFDCNTKQPGCTNVCYDNYFPISNIRLWALQLIFVTCPSLLVILHVAYREERERRHRQKHGDQCAKLYDNAGKKHGGLWWTYLFSLIFKLIIEFLFLYLLHTLWHGFNMPRLVQCANVAPCPNIVDCYIARPTEKKIFTYFMVGASAVCIVLTICELCYLICHRVLRGLHKDKPRGGCSPSSSASRASTCRCHHKLVEAGEVDPDPGNNKLQASAPNLTPI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Dog
90
Rabbit
67
Horse
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75712 As Substrate

Site PTM Type Enzyme
S228 Phosphorylation
K259 Ubiquitination
S263 Phosphorylation
T268 Phosphorylation

Research Backgrounds

Function:

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Cell junction>Gap junction.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

A connexon is composed of a hexamer of connexins. Interacts with CNST (By similarity).

Family&Domains:

Belongs to the connexin family. Beta-type (group I) subfamily.

Restrictive clause

 

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