IGFBP1 Antibody - #DF7130

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Product: IGFBP1 Antibody
Catalog: DF7130
Description: Rabbit polyclonal antibody to IGFBP1
Application: WB IHC
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Prediction: Horse, Rabbit, Dog, Xenopus
Mol.Wt.: 28kDa; 28kD(Calculated).
Uniprot: P08833
RRID: AB_2839084

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:100
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Horse(92%), Rabbit(83%), Dog(83%), Xenopus(83%)
Clonality:
Polyclonal
Specificity:
IGFBP1 Antibody detects endogenous levels of total IGFBP1.
RRID:
AB_2839084
Cite Format: Affinity Biosciences Cat# DF7130, RRID:AB_2839084.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AFBP; Alpha pregnancy associated endometrial globulin; Amniotic fluid binding protein; Binding protein 25; Binding protein 26; Binding protein 28; Growth hormone independent binding protein; hIGFBP 1; hIGFBP1; IBP 1; IBP-1; IBP1; IBP1_HUMAN; IGF binding protein 1; IGF BP25; IGF-binding protein 1; IGFBP 1; IGFBP-1; IGFBP1; Insulin like growth factor binding protein 1; Insulin-like growth factor-binding protein 1; Placental protein 12; PP 12; PP12;

Immunogens

Immunogen:

A synthesized peptide derived from human IGFBP1, corresponding to a region within C-terminal amino acids.

Uniprot:

P08833(IBP1_HUMAN) >>Visit HPA database.

Gene(ID):
IGFBP1(3484)
Description:
This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors.
Sequence:
MSEVPVARVWLVLLLLTVQVGVTAGAPWQCAPCSAEKLALCPPVSASCSEVTRSAGCGCCPMCALPLGAACGVATARCARGLSCRALPGEQQPLHALTRGQGACVQESDASAPHAAEAGSPESPESTEITEEELLDNFHLMAPSEEDHSILWDAISTYDGSKALHVTNIKKWKEPCRIELYRVVESLAKAQETSGEEISKFYLPNCNKNGFYHSRQCETSMDGEAGLCWCVYPWNGKRIPGSPEIRGDPNCQIYFNVQN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
92
Dog
83
Xenopus
83
Rabbit
83
Sheep
73
Chicken
67
Pig
64
Bovine
64
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration.

PTMs:

Phosphorylated; probably by casein kinase II. Phosphorylation alters the affinity of the protein for IGFs. In amniotic fluid, the unmodified protein is the most abundant form, while mono-, bi-, tri- and tetraphosphorylated forms are present in decreasing amounts. The phosphorylation state may influence the propensity to proteolysis.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location

References

1). The Enzyme 15-Hydroxyprostaglandin Dehydrogenase Inhibits a Shift to the Mesenchymal Pattern of Trophoblasts and Decidual Stromal Cells Accompanied by Prostaglandin Transporter in Preeclampsia. International journal of molecular sciences, 2023 (PubMed: 36982197) [IF=5.6]

Application: WB    Species: Rat    Sample:

Figure 5. Function of 15-PGDH. (A) PGE2 levels in cell lysates were quantified by ELISA. The results are representative of at least three independent experiments. (B,C) F-actin staining revealed cytoskeletal changes in dhESCs and HTR8 cells in the NC (normal control) group and SW033291 group. (D,E) Western blot assays show the relative levels of EMT markers and HLA-G in Jeg3. (F,G) Western blot assays show the relative levels of EMT markers and decidualization markers in hESC. Each experiment was independently performed three times. CTB: cytotrophoblast; EVT: extravillous trophoblasts; EMT: epithelial–mesenchymal transition; DSC: decidual stromal cell. The experiment was repeated 3 times independently. Band intensities were quantified and normalized to the GAPDH values. Values are the mean ± SD.
2). A prospective diagnostic and prognostic biomarker for hepatocellular carcinoma that functions in glucose metabolism regulation: Solute carrier family 37 member 3. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 2023 (PubMed: 36773462) [IF=4.2]
3). O-Fucosylation of BMP1 promotes endometrial decidualization by activating BMP/Smad signaling pathway. Biology of reproduction, 2023 (PubMed: 37338142) [IF=3.1]

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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