IGFBP7 Antibody | Affinity Biosciences

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IGFBP7 Antibody - Western blot analysis of extracts from COLO205 cells(heat-shock treatment), using IGFBP7 Antibody at 1/1000 dilution.

IGFBP7 Antibody - Western blot analysis of extracts from EC304 cells(heat-shock treatment), using IGFBP7 Antibody at 1/1000 dilution.

IGFBP7 Antibody - DF7131 staining Hela cells by IF/ICC.

IGFBP7 Antibody - Figure 2 SM22α loss contributes to interaction of VSMCs with macrophages via expression of VCAM-1.

Product:	IGFBP7 Antibody
Catalog:	DF7131
Description:	Rabbit polyclonal antibody to IGFBP7
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Sheep, Rabbit, Dog
Mol.Wt.:	29kDa; 29kD(Calculated).
Uniprot:	Q16270
RRID:	AB_2839085

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:100, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Bovine(100%), Sheep(100%), Rabbit(92%), Dog(92%)

Clonality:

Polyclonal

Specificity:

IGFBP7 Antibody detects endogenous levels of total IGFBP7.

RRID:

AB_2839085
Cite Format: Affinity Biosciences Cat# DF7131, RRID:AB_2839085.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AGM; Angiomodulin; FSTL2; IBP-7; IBP7; IBP7_HUMAN; IGF binding protein 7; IGF-binding protein 7; IGFBP rP1; IGFBP-7; IGFBP-rP1; IGFBP7; IGFBPRP1; Insulin like growth factor binding protein 7; Insulin-like growth factor-binding protein 7; MAC25; MAC25 protein; PGI2 stimulating factor; PGI2-stimulating factor; Prostacyclin stimulating factor; Prostacyclin-stimulating factor; PSF; RAMSVPS; TAF; Tumor-derived adhesion factor;

Immunogens

Immunogen:

Uniprot:

Q16270(IBP7_HUMAN) >>Visit HPA database.

Gene(ID):

IGFBP7(3490)

Description:

This gene encodes a member of the insulin-like growth factor (IGF)-binding protein (IGFBP) family. IGFBPs bind IGFs with high affinity, and regulate IGF availability in body fluids and tissues and modulate IGF binding to its receptors. This protein binds IGF-I and IGF-II with relatively low affinity, and belongs to a subfamily of low-affinity IGFBPs. It also stimulates prostacyclin production and cell adhesion. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and one variant has been associated with retinal arterial macroaneurysm.

Sequence:

Q16270 IBP7_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MERPSLRALLLGAAGLLLLLLPLSSSSSSDTCGPCEPASCPPLPPLGCLLGETRDACGCCPMCARGEGEPCGGGGAGRGYCAPGMECVKSRKRRKGKAGAAAGGPGVSGVCVCKSRYPVCGSDGTTYPSGCQLRAASQRAESRGEKAITQVSKGTCEQGPSIVTPPKDIWNVTGAQVYLSCEVIGIPTPVLIWNKVKRGHYGVQRTELLPGDRDNLAIQTRGGPEKHEVTGWVLVSPLSKEDAGEYECHASNSQGQASASAKITVVDALHEIPVKKGEGAEL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

100

Sheep

100

Dog

Rabbit

Chicken

Pig

Horse

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q16270 As Substrate

Q16270

Site	PTM Type	Source
K114	Ubiquitination	Uniprot
Y117	Phosphorylation	Uniprot
S122	Phosphorylation	Uniprot
S137	Phosphorylation	Uniprot
S142	Phosphorylation	Uniprot
K146	Ubiquitination	Uniprot
K153	Ubiquitination	Uniprot
N171	N-Glycosylation	Uniprot
Y201	Phosphorylation	Uniprot
T206	O-Glycosylation	Uniprot
T220	O-Glycosylation	Uniprot
T230	O-Glycosylation	Uniprot
S236	O-Glycosylation	Uniprot
S239	Phosphorylation	Uniprot
S253	Phosphorylation	Uniprot
T264	O-Glycosylation	Uniprot

Research Backgrounds

Q16270_IBP7_HUMAN

Function:

Binds IGF-I and IGF-II with a relatively low affinity. Stimulates prostacyclin (PGI2) production. Stimulates cell adhesion.

PTMs:

N-glycosylated.

Subcellular Location:

Secreted.

Subunit Structure:

May interact with VPS24/CHMP3; the relevance of such interaction however remains unclear.

References

1). SM22α Loss Contributes to Apoptosis of Vascular Smooth Muscle Cells via Macrophage-Derived circRasGEF1B. Oxidative Medicine and Cellular Longevity, 2021 (PubMed: 33859778)

Application: WB Species: Mice Sample: VSMCs

Figure 2 SM22α loss contributes to interaction of VSMCs with macrophages via expression of VCAM-1. (a) Relative quantification for RAW264.7 cell migration induced by the CM from WT and Sm22α−/− VSMCs treated with (+) or without (-) Ang II. (b) The fluorescent intensity quantification of calcein-AM-labeled RAW264.7 cell adhesion to WT or Sm22α−/− VSMCs. (c) qRT-PCR of the mRNA of TNF-α, MCP-1, IL-6, and IL-1β in RAW264.7 cells stimulated by CM from WT or Sm22α−/− VSMCs. (d) The relative number of RAW264.7 cell adhesion to Ad-vector- or Ad-SM22α-infected Sm22α−/− VSMCs. (e) Relative quantification for RAW264.7 cell migration induced by the CM from Ad-vector- or Ad-SM22α-infected Sm22α−/− VSMCs. (f) qRT-PCR of the mRNA of TNF-α, MCP-1, IL-6, and IL-1β in RAW264.7 cells stimulated by CM from Ad-vector- or Ad-SM22α-infected Sm22α−/− VSMCs. (g) Western blot analysis of differentially expressed adhesion molecules in CM from WT or Sm22α−/− VSMCs. (h, i) The fluorescent intensity quantification of calcein-AM-labeled RAW264.7 cell adhesion to Sm22α−/− VSMCs transfected with si-VCAM-1 (h) or preincubated with IgG or VCAM-1 neutralizing antibody (i). Data are presented as mean ± SD of three independent experiments. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001.

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IGFBP7 Antibody - #DF7131