CCNB1 Antibody - #BF0062
*The optimal dilutions should be determined by the end user.
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# BF0062, RRID:AB_2833855.
CCNB 1; CCNB; ccnb1; CCNB1_HUMAN; Cyclin B1; G2 mitotic specific cyclin B1; G2/mitotic-specific cyclin-B1;
Purified recombinant fragment of human CCNB1 expressed in E. Coli.
PTMs - P14635 As Substrate
|S126||Phosphorylation||P06493 (CDK1) , P28482 (MAPK1) , P53350 (PLK1)||Uniprot|
|S128||Phosphorylation||P06493 (CDK1) , P53350 (PLK1) , P28482 (MAPK1)||Uniprot|
|S133||Phosphorylation||P53350 (PLK1) , Q9H4B4 (PLK3)||Uniprot|
PTMs - P14635 As Enzyme
Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site.
Cytoplasm. Nucleus. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Binds HEI10. Interacts with catalytically active RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase. Interacts with CCNF; interaction is required for nuclear localization. Interacts with CDK5RAP3. Interacts with RFPL4A and UBE2A (By similarity). Interacts with INCA1.
Belongs to the cyclin family. Cyclin AB subfamily.
· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.
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