PIBF1 Antibody - #DF7171
| Product: | PIBF1 Antibody |
| Catalog: | DF7171 |
| Description: | Rabbit polyclonal antibody to PIBF1 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Horse, Sheep, Rabbit, Dog |
| Mol.Wt.: | 90kDa; 90kD(Calculated). |
| Uniprot: | Q8WXW3 |
| RRID: | AB_2839123 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF7171, RRID:AB_2839123.
Fold/Unfold
C13orf24; KIAA1008; PIBF 1; PIBF; PIBF1; PIBF1_HUMAN; Progesterone immunomodulatory binding factor 1; Progesterone induced blocking factor 1; Progesterone-induced-blocking factor 1; RP11 505F3.1;
Immunogens
Expressed at highest levels in testis. Moderate expression is detected in spleen, thymus, prostate, ovary, small intestine, and colon (PubMed:11935316). Expressed in the first trimester pregnancy decidua (PubMed:12516630). Localized to extravillous cytotrophoblast (at protein level). Also found in syncytiotrophoblast and part of the villous cytotrophoblast. Isoform 1 is expressed in first trimester and term villous trophoblast; trophoblast cells can additionally express other isoforms (PubMed:18817979). Overexpressed in solid tumors from stomach and uterus and in cells from ovary, cervical, breast, lymphoma and leukemia cancer (PubMed:25218441).
- Q8WXW3 PIBF1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSRKISKESKKVNISSSLESEDISLETTVPTDDISSSEEREGKVRITRQLIERKELLHNIQLLKIELSQKTMMIDNLKVDYLTKIEELEEKLNDALHQKQLLTLRLDNQLAFQQKDASKYQELMKQEMETILLRQKQLEETNLQLREKAGDVRRNLRDFELTEEQYIKLKAFPEDQLSIPEYVSVRFYELVNPLRKEICELQVKKNILAEELSTNKNQLKQLTETYEEDRKNYSEVQIRCQRLALELADTKQLIQQGDYRQENYDKVKSERDALEQEVIELRRKHEILEASHMIQTKERSELSKEVVTLEQTVTLLQKDKEYLNRQNMELSVRCAHEEDRLERLQAQLEESKKAREEMYEKYVASRDHYKTEYENKLHDELEQIRLKTNQEIDQLRNASREMYERENRNLREARDNAVAEKERAVMAEKDALEKHDQLLDRYRELQLSTESKVTEFLHQSKLKSFESERVQLLQEETARNLTQCQLECEKYQKKLEVLTKEFYSLQASSEKRITELQAQNSEHQARLDIYEKLEKELDEIIMQTAEIENEDEAERVLFSYGYGANVPTTAKRRLKQSVHLARRVLQLEKQNSLILKDLEHRKDQVTQLSQELDRANSLLNQTQQPYRYLIESVRQRDSKIDSLTESIAQLEKDVSNLNKEKSALLQTKNQMALDLEQLLNHREELAAMKQILVKMHSKHSENSLLLTKTEPKHVTENQKSKTLNVPKEHEDNIFTPKPTLFTKKEAPEWSKKQKMKT
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8WXW3 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S68 | Phosphorylation | Uniprot | |
| T71 | Phosphorylation | Uniprot | |
| K84 | Ubiquitination | Uniprot | |
| K91 | Ubiquitination | Uniprot | |
| K99 | Ubiquitination | Uniprot | |
| K115 | Ubiquitination | Uniprot | |
| K119 | Ubiquitination | Uniprot | |
| K125 | Ubiquitination | Uniprot | |
| K136 | Ubiquitination | Uniprot | |
| K168 | Ubiquitination | Uniprot | |
| K204 | Ubiquitination | Uniprot | |
| K205 | Ubiquitination | Uniprot | |
| K216 | Ubiquitination | Uniprot | |
| K220 | Ubiquitination | Uniprot | |
| K231 | Ubiquitination | Uniprot | |
| K251 | Ubiquitination | Uniprot | |
| K284 | Ubiquitination | Uniprot | |
| K304 | Ubiquitination | Uniprot | |
| K318 | Ubiquitination | Uniprot | |
| K352 | Ubiquitination | Uniprot | |
| K376 | Ubiquitination | Uniprot | |
| K387 | Ubiquitination | Uniprot | |
| K434 | Ubiquitination | Uniprot | |
| R441 | Methylation | Uniprot | |
| K452 | Ubiquitination | Uniprot | |
| K461 | Ubiquitination | Uniprot | |
| K463 | Ubiquitination | Uniprot | |
| K490 | Ubiquitination | Uniprot | |
| K494 | Ubiquitination | Uniprot | |
| K532 | Ubiquitination | Uniprot | |
| K571 | Ubiquitination | Uniprot | |
| K589 | Ubiquitination | Uniprot | |
| S592 | Phosphorylation | Uniprot | |
| K596 | Ubiquitination | Uniprot | |
| K602 | Ubiquitination | Uniprot | |
| K639 | Ubiquitination | Uniprot | |
| K652 | Ubiquitination | Uniprot | |
| S697 | Phosphorylation | Uniprot | |
| K698 | Ubiquitination | Uniprot | |
| S700 | Phosphorylation | Uniprot | |
| S703 | Phosphorylation | Uniprot | |
| K708 | Ubiquitination | Uniprot | |
| K721 | Acetylation | Uniprot | |
| K721 | Ubiquitination | Uniprot | |
| K727 | Acetylation | Uniprot | |
| K737 | Ubiquitination | Uniprot |
Research Backgrounds
Plays a role in ciliogenesis.
Pericentriolar protein required to maintain mitotic spindle pole integrity. Required for the centrosomal accumulation of PCM1 and the recruitment of centriolar satellite proteins such as BBS4. Via association with PCM1 may be involved in primary cilia formation. Required for CEP63 centrosomal localization and its interaction with WDR62. Together with CEP63 promotes centriole duplication. Promotes the centrosomal localization of CDK2.
The secreted form is a mediator of progesterone that by acting on the phospholipase A2 enzyme interferes with arachidonic acid metabolism, induces a Th2 biased immune response, and by controlling decidual naturakl killer cells (NK) activity exerts an anti-abortive effect. Increases the production of Th2-type cytokines by signaling via the JAK/STAT pathway. Activates STAT6 and inhibits STAT4 phosphorylation. Signaling via a not identified receptor seems to implicate IL4R and a GPI-anchored protein.
Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm. Secreted.
Note: In progesterone-treated astrocytoma cells a 57 kDa protein and isoform 1 (90 kDa) have been described, both being located in the intracellular medium and secreted. Respective predominant forms are isoform 1 in the intracellular and the 57 kDa protein in the extracellular medium (PubMed:25218441).
Nucleus. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriolar satellite. Secreted.
Note: Localizes to centriolar satellites throughout the cell cycle.
Secreted.
Note: Secreted by progesterone-treated lymphocytes (PubMed:14634107).
Expressed at highest levels in testis. Moderate expression is detected in spleen, thymus, prostate, ovary, small intestine, and colon. Expressed in the first trimester pregnancy decidua. Localized to extravillous cytotrophoblast (at protein level). Also found in syncytiotrophoblast and part of the villous cytotrophoblast. Isoform 1 is expressed in first trimester and term villous trophoblast; trophoblast cells can additionally express other isoforms. Overexpressed in solid tumors from stomach and uterus and in cells from ovary, cervical, breast, lymphoma and leukemia cancer.
Isoform 1 interacts with PCM1, BBS4 and CEP63. Interacts with IL4R.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.