Peroxiredoxin 3 Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/4

Peroxiredoxin 3 Antibody - Western blot analysis of extracts from rat brain, rat spleen, mouse spleen, using PRDX3 Antibody.

Peroxiredoxin 3 Antibody - DF7174 at 1/100 staining Mouse testis tissue by IHC-P.

Peroxiredoxin 3 Antibody - DF7174 at 1/100 staining Rat kidney tissue by IHC-P.

Peroxiredoxin 3 Antibody - DF7174 staining Hela cells by IF/ICC.

Product:	Peroxiredoxin 3 Antibody
Catalog:	DF7174
Description:	Rabbit polyclonal antibody to Peroxiredoxin 3
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.:	28kDa; 28kD(Calculated).
Uniprot:	P30048
RRID:	AB_2839126

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(100%), Bovine(91%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(91%), Xenopus(91%)

Clonality:

Polyclonal

Specificity:

Peroxiredoxin 3 Antibody detects endogenous levels of total Peroxiredoxin 3.

RRID:

AB_2839126
Cite Format: Affinity Biosciences Cat# DF7174, RRID:AB_2839126.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Antioxidant protein 1; AOP 1; AOP-1; AOP1; HBC189; MER5; MGC104387; MGC24293; mitochondrial; peroxiredoxin 3; Peroxiredoxin III; Peroxiredoxin-3; PRDX3; PRDX3_HUMAN; PRO1748; Protein MER5 homolog; PRX III; Prx-III; PRX3; SP 22; SP-22; SP22; Thioredoxin dependent peroxide reductase mitochondrial; Thioredoxin-dependent peroxide reductase;

Immunogens

Immunogen:

Uniprot:

P30048(PRDX3_HUMAN) >>Visit HPA database.

Gene(ID):

PRDX3(10935)

Description:

This gene encodes a protein with antioxidant function and is localized in the mitochondrion. This gene shows significant nucleotide sequence similarity to the gene coding for the C22 subunit of Salmonella typhimurium alkylhydroperoxide reductase. Expression of this gene product in E. coli deficient in the C22-subunit gene rescued resistance of the bacteria to alkylhydroperoxide. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologues suggest that these genes consist of a family that is responsible for regulation of cellular proliferation, differentiation, and antioxidant functions. Two transcript variants encoding two different isoforms have been found for this gene.

Sequence:

P30048 PRDX3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAAVGRLLRASVARHVSAIPWGISATAALRPAACGRTSLTNLLCSGSSQAKLFSTSSSCHAPAVTQHAPYFKGTAVVNGEFKDLSLDDFKGKYLVLFFYPLDFTFVCPTEIVAFSDKANEFHDVNCEVVAVSVDSHFSHLAWINTPRKNGGLGHMNIALLSDLTKQISRDYGVLLEGSGLALRGLFIIDPNGVIKHLSVNDLPVGRSVEETLRLVKAFQYVETHGEVCPANWTPDSPTIKPSPAASKEYFQKVNQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Sheep

100

Zebrafish

100

Rabbit

100

Bovine

Dog

Xenopus

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P30048 As Substrate

P30048

Site	PTM Type	Enzyme	Source
Y71	Phosphorylation		Uniprot
K83	Acetylation		Uniprot
K83	Ubiquitination		Uniprot
S86	Phosphorylation		Uniprot
K91	Acetylation		Uniprot
K91	Ubiquitination		Uniprot
K93	Acetylation		Uniprot
T146	Phosphorylation	Q5S007 (LRRK2)	Uniprot
K149	Ubiquitination		Uniprot
Y172	Phosphorylation		Uniprot
S179	Phosphorylation		Uniprot
K196	Acetylation		Uniprot
K196	Sumoylation		Uniprot
K196	Ubiquitination		Uniprot
S199	Phosphorylation		Uniprot
R207	Methylation		Uniprot
R214	Methylation		Uniprot
C229	S-Nitrosylation		Uniprot
T234	Phosphorylation		Uniprot
S237	Phosphorylation		Uniprot
K241	Ubiquitination		Uniprot
K248	Acetylation		Uniprot
K253	Ubiquitination		Uniprot

Research Backgrounds

P30048_PRDX3_HUMAN

Function:

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides. Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.

PTMs:

Phosphorylated by LRRK2; phosphorylation reduces perodixase activity.

The enzyme can be inactivated by further oxidation of the cysteine sulfenic acid (C(P)-SOH) to sulphinic acid (C(P)-SO2H) and sulphonic acid (C(P)-SO3H) instead of its condensation to a disulfide bond.

Subcellular Location:

Mitochondrion. Cytoplasm. Early endosome.
Note: Localizes to early endosomes in a RPS6KC1-dependent manner.

Subunit Structure:

Homodimer; disulfide-linked, upon oxidation. 6 homodimers assemble to form a ring-like dodecamer. Interacts with NEK6. Interacts with LRRK2. Interacts with MAP3K13. Interacts with RPS6KC1 (via PX domain).

Family&Domains:

Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily.

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Peroxiredoxin 3 Antibody - #DF7174