SLC1A4 Antibody | Affinity Biosciences

WB
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Cites

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SLC1A4 Antibody - Western blot analysis of extracts from DU145 cells, using SLC1A4 Antibody.

SLC1A4 Antibody - DF7178 at 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P.

SLC1A4 Antibody - DF7178 at 1/100 staining Mouse heart tissue by IHC-P.

SLC1A4 Antibody - DF7178 at 1/100 staining Mouse muscle tissue by IHC-P.

SLC1A4 Antibody - DF7178 at 1/100 staining Rat muscle tissue by IHC-P.

SLC1A4 Antibody - DF7178 at 1/100 staining Rat skin tissue by IHC-P.

SLC1A4 Antibody - DF7178 staining Hela cells by IF/ICC.

SLC1A4 Antibody - Figure 3 Effects of metformin on MuRF1 and MAFbx expression.

Product:	SLC1A4 Antibody
Catalog:	DF7178
Description:	Rabbit polyclonal antibody to SLC1A4
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	56kDa; 56kD(Calculated).
Uniprot:	P43007
RRID:	AB_2839130

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(92%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%)

Clonality:

Polyclonal

Specificity:

SLC1A4 Antibody detects endogenous levels of total SLC1A4.

RRID:

AB_2839130
Cite Format: Affinity Biosciences Cat# DF7178, RRID:AB_2839130.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Alanine/serine/cysteine/threonine transporter 1; ASCT-1; ASCT1; AW045657; Glutamate/neutral amino acid transporter; Neutral amino acid transporter A; OTTHUMP00000159933; OTTHUMP00000235138; SATT; SATT_HUMAN; SLC1A4; Solute carrier family 1 (glutamate/neutral amino acid transporter), member 4; Solute carrier family 1 member 4;

Immunogens

Immunogen:

Uniprot:

P43007(SATT_HUMAN) >>Visit HPA database.

Gene(ID):

SLC1A4(6509)

Expression:

P43007 SATT_HUMAN:

Expressed mostly in brain, muscle, and pancreas but detected in all tissues examined.

Description:

SLC1A4, also known as ASCT1, is a neutral amino acid transporter. Its other name, ASCT1, was given because it mediates obligatory exchange of alanine, serine, cysteine, and threonine (1). SLC1A4 mediates the efflux of glutamate from the neuron into the synaptic junction via calcium-independent release, as well as mediating the efflux of L-serine from glial cells and its uptake by neurons (2). SLC1A4-mediated transport is shown to involve a symmetrical potassium-independent electroneutral exchange of neutral amino acids and sodium, such that the current activated during transport is carried only by chloride ions (3).

Sequence:

P43007 SATT_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEKSNETNGYLDSAQAGPAAGPGAPGTAAGRARRCAGFLRRQALVLLTVSGVLAGAGLGAALRGLSLSRTQVTYLAFPGEMLLRMLRMIILPLVVCSLVSGAASLDASCLGRLGGIAVAYFGLTTLSASALAVALAFIIKPGSGAQTLQSSDLGLEDSGPPPVPKETVDSFLDLARNLFPSNLVVAAFRTYATDYKVVTQNSSSGNVTHEKIPIGTEIEGMNILGLVLFALVLGVALKKLGSEGEDLIRFFNSLNEATMVLVSWIMWYVPVGIMFLVGSKIVEMKDIIVLVTSLGKYIFASILGHVIHGGIVLPLIYFVFTRKNPFRFLLGLLAPFATAFATCSSSATLPSMMKCIEENNGVDKRISRFILPIGATVNMDGAAIFQCVAAVFIAQLNNVELNAGQIFTILVTATASSVGAAGVPAGGVLTIAIILEAIGLPTHDLPLILAVDWIVDRTTTVVNVEGDALGAGILHHLNQKATKKGEQELAEVKVEAIPNCKSEEETSPLVTHQNPAGPVASAPELESKESVL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Zebrafish

Chicken

Xenopus

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P43007 As Substrate

P43007

Site	PTM Type	Source
M1	Acetylation	Uniprot
T7	Phosphorylation	Uniprot
Y10	Phosphorylation	Uniprot
K196	Ubiquitination	Uniprot
N201	N-Glycosylation	Uniprot
N206	N-Glycosylation	Uniprot
S242	Phosphorylation	Uniprot
K483	Ubiquitination	Uniprot
K484	Ubiquitination	Uniprot
K493	Ubiquitination	Uniprot
K501	Ubiquitination	Uniprot
S502	Phosphorylation	Uniprot
T506	Phosphorylation	Uniprot
S507	Phosphorylation	Uniprot
S521	Phosphorylation	Uniprot
S527	Phosphorylation	Uniprot
K528	Ubiquitination	Uniprot
S530	Phosphorylation	Uniprot

Research Backgrounds

P43007_SATT_HUMAN

Function:

Transporter for alanine, serine, cysteine, and threonine. Exhibits sodium dependence.

Subcellular Location:

Membrane>Multi-pass membrane protein. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Tissue Specificity:

Expressed mostly in brain, muscle, and pancreas but detected in all tissues examined.

Family&Domains:

Belongs to the dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family. SLC1A4 subfamily.

References

1). Metformin attenuates angiotensin II‑induced cardiomyocyte hypertrophy by upregulating the MuRF1 and MAFbx pathway. Experimental and Therapeutic Medicine, 2021 (PubMed: 34539827) [IF=2.4]

Application: WB Species: Rat Sample: H9c2 cells

Figure 3 Effects of metformin on MuRF1 and MAFbx expression. (A) Western blot analysis measuring the expression of MuRF1 and MAFbx protein after the indicated treatments. (B) Densitometric analysis of MuRF1 and MAFbx expression in cardiomyocytes. n=3 for each group. *P<0.05. #P<0.05. MuRF1, muscle RING-finger protein-1; MAFbx, muscle atrophy F-box; Ang, angiotensin; Met, metformin.

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SLC1A4 Antibody - #DF7178