TRIM63 Antibody - #DF7187

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Product: TRIM63 Antibody
Catalog: DF7187
Description: Rabbit polyclonal antibody to TRIM63
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 40kDa; 40kD(Calculated).
Uniprot: Q969Q1
RRID: AB_2839139

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:100, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(82%), Dog(100%)
Clonality:
Polyclonal
Specificity:
TRIM63 Antibody detects endogenous levels of total TRIM63.
RRID:
AB_2839139
Cite Format: Affinity Biosciences Cat# DF7187, RRID:AB_2839139.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

E3 ubiquitin-protein ligase TRIM63; FLJ32380; IRF; Iris RING finger protein; MURF 1; MURF-1; MuRF1; MURF2; Muscle specific ring finger protein 1; Muscle specific ring finger protein 2; Muscle-specific RING finger protein 1; OTTHUMP00000008701; RING finger protein 28; RNF 28; RNF28; SMRZ; Striated muscle RING zinc finger protein; TRI63_HUMAN; TRIM 63; Trim63; Tripartite motif containing 63; tripartite motif containing 63, E3 ubiquitin protein ligase; Tripartite motif containing protein 63; Tripartite motif-containing protein 63; Ubiquitin ligase TRIM63;

Immunogens

Immunogen:
Uniprot:

Q969Q1(TRI63_HUMAN) >>Visit HPA database.

Gene(ID):
TRIM63(84676)
Expression:
Q969Q1 TRI63_HUMAN:

Muscle specific. Selectively expressed in heart and skeletal muscle. Also expressed in the iris.

Description:
This gene encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is an E3 ubiquitin ligase that localizes to the Z-line and M-line lattices of myofibrils. This protein plays an important role in the atrophy of skeletal and cardiac muscle and is required for the degradation of myosin heavy chain proteins, myosin light chain, myosin binding protein, and for muscle-type creatine kinase.
Sequence:
MDYKSSLIQDGNPMENLEKQLICPICLEMFTKPVVILPCQHNLCRKCANDIFQAANPYWTSRGSSVSMSGGRFRCPTCRHEVIMDRHGVYGLQRNLLVENIIDIYKQECSSRPLQKGSHPMCKEHEDEKINIYCLTCEVPTCSMCKVFGIHKACEVAPLQSVFQGQKTELNNCISMLVAGNDRVQTIITQLEDSRRVTKENSHQVKEELSQKFDTLYAILDEKKSELLQRITQEQEKKLSFIEALIQQYQEQLDKSTKLVETAIQSLDEPGGATFLLTAKQLIKSIVEASKGCQLGKTEQGFENMDFFTLDLEHIADALRAIDFGTDEEEEEFIEEEDQEEEESTEGKEEGHQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
82
Chicken
45
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q969Q1 As Substrate

Site PTM Type Enzyme
S64 Phosphorylation
S67 Phosphorylation
S69 Phosphorylation

Research Backgrounds

Function:

E3 ubiquitin ligase. Mediates the ubiquitination and subsequent proteasomal degradation of CKM, GMEB1 and HIBADH. Regulates the proteasomal degradation of muscle proteins under amino acid starvation, where muscle protein is catabolized to provide other organs with amino acids. Inhibits de novo skeletal muscle protein synthesis under amino acid starvation. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. May play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. May regulate the organization of myofibrils through TTN in muscle cells.

Subcellular Location:

Cytoplasm. Nucleus. Cytoplasm>Myofibril>Sarcomere>M line. Cytoplasm>Myofibril>Sarcomere>Z line.
Note: Colocalizes with TNNI3 in myocytes (By similarity). Localizes to the M- and Z-lines in skeletal muscle.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Muscle specific. Selectively expressed in heart and skeletal muscle. Also expressed in the iris.

Subunit Structure:

Homodimer. Homooligomer and heterooligomer. Interacts with SUMO2, titin/TTN and GMEB1. Interacts with TRIM54 and probably with TRIM55 and TNNI3. Forms a ternary complex with RACK1 and PRKCE (By similarity). Interacts with CKM.

Family&Domains:

The RING-type zinc finger mediates interaction with SUMO2 and localization to the nucleus. Also required for the E3 ubiquitin ligase activity (By similarity).

The B box-type zinc finger mediates homodimerization.

References

1). Kcnma1 is involved in mitochondrial homeostasis in diabetes-related skeletal muscle atrophy. The FASEB Journal, 2023 (PubMed: 36929614) [IF=4.4]
2). Melatonin protects against sarcopenia in middle-aged mice. Histology and histopathology, 2024 (PubMed: 39385610) [IF=2.5]

Application: WB    Species: Mouse    Sample: GA tissues

Figure 4. The effects of MEL on the PGC-1α/TFAM pathway in GA tissues of middle-aged mice a-c. Western blot and qRT-PCR were applied to detect the effects of MEL on PGC-1α/TFAM pathwayrelated protein and mRNA levels, including cytochrome c oxidase subunit 4 (COX4), cystatin C (CYTC), nuclear respiratory factor 1 (NRF-1), mitochondrial transcription factor A (TFAM), p-P38, P38, and peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α), n=3. Data are manifested as mean ± SD * P
3). A Nanocurcumin and Pyrroloquinoline Quinone Formulation Prevents Hypobaric Hypoxia-Induced Skeletal Muscle Atrophy by Modulating NF-κB Signaling Pathway. HIGH ALTITUDE MEDICINE & BIOLOGY, 2022 (PubMed: 35384739) [IF=1.6]

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