MYSM1 Antibody - #DF7188

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Product: MYSM1 Antibody
Catalog: DF7188
Description: Rabbit polyclonal antibody to MYSM1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 95kD; 95kD(Calculated).
Uniprot: Q5VVJ2
RRID: AB_2839140

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:100, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
MYSM1 Antibody detects endogenous levels of total MYSM1.
RRID:
AB_2839140
Cite Format: Affinity Biosciences Cat# DF7188, RRID:AB_2839140.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

2A-DUB; 2ADUB; Histone H2A deubiquitinase; Histone H2A deubiquitinase MYSM1; KIAA1915; Myb like SWIRM and MPN domains 1; Myb like, SWIRM and MPN domain containing protein; Myb like, SWIRM and MPN domains 1; Myb-like; Myb-like, SWIRM and MPN domains 1; MYSM 1; mysm1; MYSM1 Myb-like, SWIRM and MPN domains 1; MYSM1_HUMAN; RP4-592A1.1; SWIRM and MPN domain-containing protein 1;

Immunogens

Immunogen:
Uniprot:

Q5VVJ2(MYSM1_HUMAN) >>Visit HPA database.

Gene(ID):
MYSM1(114803)
Sequence:
MAAEEADVDIEGDVVAAAGAQPGSGENTASVLQKDHYLDSSWRTENGLIPWTLDNTISEENRAVIEKMLLEEEYYLSKKSQPEKVWLDQKEDDKKYMKSLQKTAKIMVHSPTKPASYSVKWTIEEKELFEQGLAKFGRRWTKISKLIGSRTVLQVKSYARQYFKNKVKCGLDKETPNQKTGHNLQVKNEDKGTKAWTPSCLRGRADPNLNAVKIEKLSDDEEVDITDEVDELSSQTPQKNSSSDLLLDFPNSKMHETNQGEFITSDSQEALFSKSSRGCLQNEKQDETLSSSEITLWTEKQSNGDKKSIELNDQKFNELIKNCNKHDGRGIIVDARQLPSPEPCEIQKNLNDNEMLFHSCQMVEESHEEEELKPPEQEIEIDRNIIQEEEKQAIPEFFEGRQAKTPERYLKIRNYILDQWEICKPKYLNKTSVRPGLKNCGDVNCIGRIHTYLELIGAINFGCEQAVYNRPQTVDKVRIRDRKDAVEAYQLAQRLQSMRTRRRRVRDPWGNWCDAKDLEGQTFEHLSAEELAKRREEEKGRPVKSLKVPRPTKSSFDPFQLIPCNFFSEEKQEPFQVKVASEALLIMDLHAHVSMAEVIGLLGGRYSEVDKVVEVCAAEPCNSLSTGLQCEMDPVSQTQASETLAVRGFSVIGWYHSHPAFDPNPSLRDIDTQAKYQSYFSRGGAKFIGMIVSPYNRNNPLPYSQITCLVISEEISPDGSYRLPYKFEVQQMLEEPQWGLVFEKTRWIIEKYRLSHSSVPMDKIFRRDSDLTCLQKLLECMRKTLSKVTNCFMAEEFLTEIENLFLSNYKSNQENGVTEENCTKELLM

PTMs - Q5VVJ2 As Substrate

Site PTM Type Enzyme
K67 Ubiquitination
Y74 Phosphorylation
Y75 Phosphorylation
K78 Ubiquitination
K79 Ubiquitination
K84 Ubiquitination
K90 Ubiquitination
K98 Ubiquitination
S110 Phosphorylation
T112 Phosphorylation
S118 Phosphorylation
K126 Ubiquitination
K135 Ubiquitination
K145 Ubiquitination
S149 Phosphorylation
K173 Ubiquitination
T175 Phosphorylation
K187 Sumoylation
T197 Phosphorylation
S218 Phosphorylation
T226 Phosphorylation
S234 Phosphorylation
T236 Phosphorylation
S242 Phosphorylation
S267 Phosphorylation
K284 Ubiquitination
K315 Ubiquitination
K321 Ubiquitination
S340 Phosphorylation
K391 Ubiquitination
T405 Phosphorylation
Y409 Phosphorylation
K411 Acetylation
K533 Ubiquitination
K675 Ubiquitination

Research Backgrounds

Function:

Metalloprotease that specifically deubiquitinates monoubiquitinated histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Preferentially deubiquitinates monoubiquitinated H2A in hyperacetylated nucleosomes. Deubiquitination of histone H2A leads to facilitate the phosphorylation and dissociation of histone H1 from the nucleosome. Acts as a coactivator by participating in the initiation and elongation steps of androgen receptor (AR)-induced gene activation. Required for correct regulation of hematopoiesis and lymphocyte differentiation.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of a large chromatin remodeling complex, at least composed of MYSM1, PCAF, RBM10 and KIF11/TRIP5. Binds histones.

Family&Domains:

Binds double-stranded DNA via the SANT domain. The SWIRM domain does not bind double-stranded DNA.

Belongs to the peptidase M67A family. MYSM1 subfamily.

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