Annexin A6 Antibody | Affinity Biosciences

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Annexin A6 Antibody - Western blot analysis of extracts from various samples, using Annexin A6 Antibody.

Annexin A6 Antibody - AF0116 at 1/100 staining Mouse brain tissue by IHC-P.

Product:	Annexin A6 Antibody
Catalog:	AF0116
Description:	Rabbit polyclonal antibody to Annexin A6
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Sheep, Dog
Mol.Wt.:	75kDa; 76kD(Calculated).
Uniprot:	P08133
RRID:	AB_2833266

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC: 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(91%), Bovine(91%), Sheep(82%), Dog(91%)

Clonality:

Polyclonal

Specificity:

Annexin A6 Antibody detects endogenous levels of total Annexin A6.

RRID:

AB_2833266
Cite Format: Affinity Biosciences Cat# AF0116, RRID:AB_2833266.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

67 kDa calelectrin; Annexin A6; Annexin VI; Annexin VI p68; Annexin-6; AnnexinA6; AnnexinVI; ANX 6; ANX A6; ANX6; ANXA 6; ANXA6; ANXA6_HUMAN; Calcium binding protein p68; Calelectrin; Calphobindin II; Calphobindin-II; CalphobindinII; CBP 68; CBP68; Chromobindin 20; Chromobindin-20; Chromobindin20; CPB II; CPB-II; CPBII; Lipocortin VI; LipocortinVI; p68; p70; Protein III; ProteinIII;

Immunogens

Immunogen:

A synthesized peptide derived from human Annexin A6, corresponding to a region within N-terminal amino acids.

Uniprot:

P08133(ANXA6_HUMAN) >>Visit HPA database.

Gene(ID):

ANXA6(309)

Description:

ANXA6 a calcium/phospholipid-binding protein that may associate with CD21. May regulate the release of Ca(2+) from intracellular stores. Phosphorylated in response to growth factor stimulation. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.

Sequence:

P08133 ANXA6_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAKPAQGAKYRGSIHDFPGFDPNQDAEALYTAMKGFGSDKEAILDIITSRSNRQRQEVCQSYKSLYGKDLIADLKYELTGKFERLIVGLMRPPAYCDAKEIKDAISGIGTDEKCLIEILASRTNEQMHQLVAAYKDAYERDLEADIIGDTSGHFQKMLVVLLQGTREEDDVVSEDLVQQDVQDLYEAGELKWGTDEAQFIYILGNRSKQHLRLVFDEYLKTTGKPIEASIRGELSGDFEKLMLAVVKCIRSTPEYFAERLFKAMKGLGTRDNTLIRIMVSRSELDMLDIREIFRTKYEKSLYSMIKNDTSGEYKKTLLKLSGGDDDAAGQFFPEAAQVAYQMWELSAVARVELKGTVRPANDFNPDADAKALRKAMKGLGTDEDTIIDIITHRSNVQRQQIRQTFKSHFGRDLMTDLKSEISGDLARLILGLMMPPAHYDAKQLKKAMEGAGTDEKALIEILATRTNAEIRAINEAYKEDYHKSLEDALSSDTSGHFRRILISLATGHREEGGENLDQAREDAQVAAEILEIADTPSGDKTSLETRFMTILCTRSYPHLRRVFQEFIKMTNYDVEHTIKKEMSGDVRDAFVAIVQSVKNKPLFFADKLYKSMKGAGTDEKTLTRIMVSRSEIDLLNIRREFIEKYDKSLHQAIEGDTSGDFLKALLALCGGED

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Bovine

Dog

Sheep

Xenopus

Chicken

Horse

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

P08133_ANXA6_HUMAN

Function:

May associate with CD21. May regulate the release of Ca(2+) from intracellular stores.

PTMs:

Phosphorylated in response to growth factor stimulation.

Subcellular Location:

Cytoplasm. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Family&Domains:

A pair of annexin repeats may form one binding site for calcium and phospholipid.

Belongs to the annexin family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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Annexin A6 Antibody - #AF0116