GMNN Antibody - #DF7270

(2)  
Product: GMNN Antibody
Catalog: DF7270
Description: Rabbit polyclonal antibody to GMNN
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Rabbit
Mol.Wt.: 23kDa; 24kD(Calculated).
Uniprot: O75496
RRID: AB_2839209

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(80%), Rabbit(80%)
Clonality:
Polyclonal
Specificity:
GMNN Antibody detects endogenous levels of total GMNN.
RRID:
AB_2839209
Cite Format: Affinity Biosciences Cat# DF7270, RRID:AB_2839209.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DNA replication inhibitor; Gem; GEMI_HUMAN; Geminin; Geminin DNA replication inhibitor; GMNN; OTTHUMP00000039393; RP3 369A17.3;

Immunogens

Immunogen:
Uniprot:

O75496(GEMI_HUMAN) >>Visit HPA database.

Gene(ID):
GMNN(51053)
Description:
This gene encodes a protein that plays a critical role in cell cycle regulation. The encoded protein inhibits DNA replication by binding to DNA replication factor Cdt1, preventing the incorporation of minichromosome maintenance proteins into the pre-replication complex. The encoded protein is expressed during the S and G2 phases of the cell cycle and is degraded by the anaphase-promoting complex during the metaphase-anaphase transition. Increased expression of this gene may play a role in several malignancies including colon, rectal and breast cancer. Alternatively spliced transcript variants have been observed for this gene, and two pseudogenes of this gene are located on the short arm of chromosome 16.
Sequence:
MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELSAGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAEKRRKALYEALKENEKLHKEIEQKDNEIARLKKENKELAEVAEHVQYMAELIERLNGEPLDNFESLDNQEFDSEEETVEDSLVEDSEIGTCAEGTVSSSTDAKPCI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
80
Pig
80
Dog
75
Horse
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75496 As Substrate

Site PTM Type Enzyme
K17 Ubiquitination
T25 Phosphorylation O14965 (AURKA)
K27 Acetylation
K27 Ubiquitination
S32 Phosphorylation
S34 Phosphorylation
S36 Phosphorylation
S45 Phosphorylation
S49 Phosphorylation
K50 Acetylation
K50 Ubiquitination
K52 Ubiquitination
T59 Phosphorylation
S60 Phosphorylation
T61 Phosphorylation
S63 Phosphorylation
S64 Phosphorylation
S72 Phosphorylation
K76 Ubiquitination
S85 Phosphorylation
K91 Ubiquitination
S95 Phosphorylation
S96 Phosphorylation
Y98 Phosphorylation
K100 Ubiquitination
K105 Acetylation
K108 Ubiquitination
K115 Ubiquitination
K122 Ubiquitination
K127 Ubiquitination
K139 Ubiquitination
Y150 Phosphorylation
S184 Phosphorylation P68400 (CSNK2A1)
S200 Phosphorylation
S201 Phosphorylation
S202 Phosphorylation
T203 Phosphorylation

Research Backgrounds

Function:

Inhibits DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle.

Inhibits the transcriptional activity of a subset of Hox proteins, enrolling them in cell proliferative control.

PTMs:

Phosphorylated during mitosis. Phosphorylation at Ser-184 by CK2 results in enhanced binding to Hox proteins and more potent inhibitory effect on Hox transcriptional activity.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Mainly cytoplasmic but can be relocalized to the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homotetramer. Interacts with CDT1; this inhibits binding of the MCM complex to origins of replication. The complex with CDT1 exists in two forms, a 'permissive' heterotrimer and an 'inhibitory' heterohexamer. Interacts (via coiled-coil domain) with IDAS (via coiled-coil domain); this targets GMNN to the nucleus. The heterodimer formed by GMNN and MCIDAS has much lower affinity for CDT1 than the GMNN homodimer. Interacts with a subset of Hox proteins, affinity increasing from anterior to posterior types, the strongest interaction being with HOXB1, HOXC9 and HOXD10. Interacts with LRWD1 from G1/S to mitosis.

Family&Domains:

Belongs to the geminin family.

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