HDGF Antibody | Affinity Biosciences

1/1

HDGF Antibody - Western blot analysis of extracts from VERO cells, using HDGF Antibody.

Product:	HDGF Antibody
Catalog:	DF7289
Description:	Rabbit polyclonal antibody to HDGF
Application:	WB IHC
Reactivity:	Human, Mouse, Rat, Monkey
Mol.Wt.:	26kDa; 27kD(Calculated).
Uniprot:	P51858
RRID:	AB_2839228

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat,Monkey

Clonality:

Polyclonal

Specificity:

HDGF Antibody detects endogenous levels of total HDGF.

RRID:

AB_2839228
Cite Format: Affinity Biosciences Cat# DF7289, RRID:AB_2839228.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

FLJ96580; HDGF; HDGF_HUMAN; Hepatoma derived growth factor; Hepatoma-derived growth factor; High mobility group protein 1 like 2; High mobility group protein 1-like 2; HMG-1L2; HMG1L2;

Immunogens

Immunogen:

Uniprot:

P51858(HDGF_HUMAN) >>Visit HPA database.

Gene(ID):

HDGF(3068)

Expression:

P51858 HDGF_HUMAN:

Ubiquitous.

Description:

This gene encodes a member of the hepatoma-derived growth factor family. The encoded protein has mitogenic and DNA-binding activity and may play a role in cellular proliferation and differentiation. This gene was thought initially to be located on chromosome X, however, that location has been determined to correspond to a related pseudogene. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Sequence:

P51858 HDGF_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSRSNRQKEYKCGDLVFAKMKGYPHWPARIDEMPEAAVKSTANKYQVFFFGTHETAFLGPKDLFPYEESKEKFGKPNKRKGFSEGLWEIENNPTVKASGYQSSQKKSCVEEPEPEPEAAEGDGDKKGNAEGSSDEEGKLVIDEPAKEKNEKGALKRRAGDLLEDSPKRPKEAENPEGEEKEAATLEVERPLPMEVEKNSTPSEPGSGRGPPQEEEEEEDEEEEATKEDAEAPGIRDHESL

PTMs - P51858 As Substrate

P51858

Site	PTM Type	Source
K11	Sumoylation	Uniprot
K11	Ubiquitination	Uniprot
C12	S-Nitrosylation	Uniprot
K21	Ubiquitination	Uniprot
Y23	Phosphorylation	Uniprot
K39	Ubiquitination	Uniprot
K44	Acetylation	Uniprot
Y45	Phosphorylation	Uniprot
Y66	Phosphorylation	Uniprot
K70	Ubiquitination	Uniprot
K80	Sumoylation	Uniprot
K80	Ubiquitination	Uniprot
S83	Phosphorylation	Uniprot
K96	Ubiquitination	Uniprot
S102	Phosphorylation	Uniprot
S103	Phosphorylation	Uniprot
K105	Acetylation	Uniprot
K105	Ubiquitination	Uniprot
K106	Acetylation	Uniprot
K106	Ubiquitination	Uniprot
S107	Phosphorylation	Uniprot
C108	S-Nitrosylation	Uniprot
K125	Acetylation	Uniprot
K126	Ubiquitination	Uniprot
S132	Phosphorylation	Uniprot
S133	Phosphorylation	Uniprot
K146	Acetylation	Uniprot
K146	Ubiquitination	Uniprot
S165	Phosphorylation	Uniprot
K167	Ubiquitination	Uniprot
K180	Acetylation	Uniprot
K180	Ubiquitination	Uniprot
T184	Phosphorylation	Uniprot
K197	Acetylation	Uniprot
K197	Ubiquitination	Uniprot
S199	Phosphorylation	Uniprot
T200	Phosphorylation	Uniprot
S202	Phosphorylation	Uniprot
S206	Phosphorylation	Uniprot
R208	Methylation	Uniprot
T225	Phosphorylation	Uniprot
K226	Ubiquitination	Uniprot
R235	Methylation	Uniprot
S239	Phosphorylation	Uniprot

Research Backgrounds

P51858_HDGF_HUMAN

Function:

Acts as a transcriptional repressor. Has mitogenic activity for fibroblasts. Heparin-binding protein.

Does not have mitogenic activity for fibroblasts. Does not bind heparin.

Has mitogenic activity for fibroblasts. Heparin-binding protein.

PTMs:

Sumoylated with SUMO1. Sumoylation prevents binding to chromatin.

Phosphorylation at Ser-165 is likely to be required for secretion.

Subcellular Location:

Nucleus. Cytoplasm. Secreted>Extracellular exosome.
Note: Secreted by exosomes and is located inside the exosome (PubMed:27926477). May also be secreted as free protein via an as yet unknown pathway (PubMed:27926477).

Nucleus. Cytoplasm. Secreted>Extracellular exosome.
Note: Secreted by exosomes and is located on the outer exosome surface.

Nucleus. Cytoplasm. Secreted>Extracellular exosome.
Note: Secreted by exosomes and is located on the outer exosome surface.

Tissue Specificity:

Ubiquitous.

Subunit Structure:

Monomer, and domain-swapped homodimer.

Interacts with nuclear proteins NCL and YBX1/YB1.

Interacts with cytoskeletal proteins such as actin, dynein, tubulin and vimentin.

Interacts with cytoskeletal proteins such as dynein and tubulin but does not interact with actin or vimentin.

Family&Domains:

The PWWP domain harbors the heparin-binding sites and is responsible for DNA-binding, while the C-terminal region is essentially unstructured.

The N-terminal region does not contain a typical signal sequence but is required for secretion (By similarity). It also determines exosomal location (PubMed:26845719).

Belongs to the HDGF family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

HDGF Antibody - #DF7289