HDGF Antibody - #DF7289

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Product: HDGF Antibody
Catalog: DF7289
Description: Rabbit polyclonal antibody to HDGF
Application: WB IHC
Reactivity: Human, Mouse, Rat, Monkey
Mol.Wt.: 26kDa; 27kD(Calculated).
Uniprot: P51858
RRID: AB_2839228

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Clonality:
Polyclonal
Specificity:
HDGF Antibody detects endogenous levels of total HDGF.
RRID:
AB_2839228
Cite Format: Affinity Biosciences Cat# DF7289, RRID:AB_2839228.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FLJ96580; HDGF; HDGF_HUMAN; Hepatoma derived growth factor; Hepatoma-derived growth factor; High mobility group protein 1 like 2; High mobility group protein 1-like 2; HMG-1L2; HMG1L2;

Immunogens

Immunogen:

A synthesized peptide derived from human HDGF, corresponding to a region within the internal amino acids.

Uniprot:

P51858(HDGF_HUMAN) >>Visit HPA database.

Gene(ID):
HDGF(3068)
Expression:
P51858 HDGF_HUMAN:

Ubiquitous.

Description:
This gene encodes a member of the hepatoma-derived growth factor family. The encoded protein has mitogenic and DNA-binding activity and may play a role in cellular proliferation and differentiation. This gene was thought initially to be located on chromosome X, however, that location has been determined to correspond to a related pseudogene. Alternatively spliced transcript variants encoding distinct isoforms have been described.
Sequence:
MSRSNRQKEYKCGDLVFAKMKGYPHWPARIDEMPEAAVKSTANKYQVFFFGTHETAFLGPKDLFPYEESKEKFGKPNKRKGFSEGLWEIENNPTVKASGYQSSQKKSCVEEPEPEPEAAEGDGDKKGNAEGSSDEEGKLVIDEPAKEKNEKGALKRRAGDLLEDSPKRPKEAENPEGEEKEAATLEVERPLPMEVEKNSTPSEPGSGRGPPQEEEEEEDEEEEATKEDAEAPGIRDHESL

Research Backgrounds

Function:

Acts as a transcriptional repressor. Has mitogenic activity for fibroblasts. Heparin-binding protein.

Does not have mitogenic activity for fibroblasts. Does not bind heparin.

Has mitogenic activity for fibroblasts. Heparin-binding protein.

PTMs:

Sumoylated with SUMO1. Sumoylation prevents binding to chromatin.

Phosphorylation at Ser-165 is likely to be required for secretion.

Subcellular Location:

Nucleus. Cytoplasm. Secreted>Extracellular exosome.
Note: Secreted by exosomes and is located inside the exosome (PubMed:27926477). May also be secreted as free protein via an as yet unknown pathway (PubMed:27926477).

Nucleus. Cytoplasm. Secreted>Extracellular exosome.
Note: Secreted by exosomes and is located on the outer exosome surface.

Nucleus. Cytoplasm. Secreted>Extracellular exosome.
Note: Secreted by exosomes and is located on the outer exosome surface.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Family&Domains:

The PWWP domain harbors the heparin-binding sites and is responsible for DNA-binding, while the C-terminal region is essentially unstructured.

The N-terminal region does not contain a typical signal sequence but is required for secretion (By similarity). It also determines exosomal location (PubMed:26845719).

Belongs to the HDGF family.

Restrictive clause

 

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