GFRA1 Antibody | Affinity Biosciences

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IHC
Cites

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Western blot analysis of extracts from mouse brain, using GFRA1 Antibody.

DF7309 at 1/100 staining Rat brain tissue by IHC-P.

Figure 6 The expression levels of TGF-β1 (a), TβRI (b), TβRII (c), p-Smad2 (d), p-Smad3 (e), and Smad7 (f) protein and their protein band ((g), (h)) in the lung tissue of mice in each group.

Product:	GFRA1 Antibody
Catalog:	DF7309
Description:	Rabbit polyclonal antibody to GFRA1
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Sheep, Dog
Mol.Wt.:	51kDa; 51kD(Calculated).
Uniprot:	P56159
RRID:	AB_2839247

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(80%), Bovine(90%), Sheep(90%), Dog(80%)

Clonality:

Polyclonal

Specificity:

GFRA1 Antibody detects endogenous levels of total GFRA1.

RRID:

AB_2839247
Cite Format: Affinity Biosciences Cat# DF7309, RRID:AB_2839247.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

GDNF family receptor alpha 1; GDNF family receptor alpha-1; GDNF R; GDNF RA; GDNF receptor alpha; GDNF receptor alpha-1; GDNFR alpha 1; GDNFR alpha; GDNFR; GDNFR-alpha-1; GDNFRA; GDNFRalpha; GFR alpha 1; GFR alpha1; GFR-alpha-1; GFRA 1; Gfra1; GFRA1_HUMAN; GFRalpha1; Glial cell line derived neurotrophic factor receptor alpha; GPI linked anchor protein; MGC23045; PI linked cell surface accessory protein; RET 1L; RET ligand 1; RET1L; RETL 1; RETL1; TGF beta related neurotrophic factor receptor 1; TGF-beta-related neurotrophic factor receptor 1; TRNR 1; TRNR1;

Immunogens

Immunogen:

Uniprot:

P56159(GFRA1_HUMAN) >>Visit HPA database.

Gene(ID):

GFRA1(2674)

Description:

Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The protein encoded by this gene is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This gene is a candidate gene for Hirschsprung disease. Multiple alternatively spliced transcript variants have been described for this gene.

Sequence:

P56159 GFRA1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MFLATLYFALPLLDLLLSAEVSGGDRLDCVKASDQCLKEQSCSTKYRTLRQCVAGKETNFSLASGLEAKDECRSAMEALKQKSLYNCRCKRGMKKEKNCLRIYWSMYQSLQGNDLLEDSPYEPVNSRLSDIFRVVPFISDVFQQVEHIPKGNNCLDAAKACNLDDICKKYRSAYITPCTTSVSNDVCNRRKCHKALRQFFDKVPAKHSYGMLFCSCRDIACTERRRQTIVPVCSYEEREKPNCLNLQDSCKTNYICRSRLADFFTNCQPESRSVSSCLKENYADCLLAYSGLIGTVMTPNYIDSSSLSVAPWCDCSNSGNDLEECLKFLNFFKDNTCLKNAIQAFGNGSDVTVWQPAFPVQTTTATTTTALRVKNKPLGPAGSENEIPTHVLPPCANLQAQKLKSNVSGNTHLCISNGNYEKEGLGASSHITTKSMAAPPSCGLSPLLVLVVTALSTLLSLTETS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

Sheep

Pig

Dog

Rabbit

Horse

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P56159 As Substrate

P56159

Site	PTM Type	Source
S183	Phosphorylation	Uniprot
T432	O-Glycosylation	Uniprot
T433	O-Glycosylation	Uniprot

Research Backgrounds

P56159_GFRA1_HUMAN

Function:

Receptor for GDNF. Mediates the GDNF-induced autophosphorylation and activation of the RET receptor (By similarity).

Subcellular Location:

Cell membrane>Lipid-anchor. Golgi apparatus>trans-Golgi network. Endosome. Endosome>Multivesicular body.
Note: Localizes mainly to the plasma membrane. In the presence of SORL1, shifts to vesicular structures, including trans-Golgi network, endosomes and multivesicular bodies.

Subunit Structure:

2 molecules of GDNFR-alpha are thought to form a complex with the disulfide-linked GDNF dimer and with 2 molecules of RET (By similarity). Interacts with RET. Interacts with SORL1, either alone or in complex with GDNF. Interaction between SORL1 and GFRA1 leads to GFRA1 internalization, but not degradation.

Family&Domains:

Belongs to the GDNFR family.

References

1). Scorpion Venom Polypeptide Inhibits Pulmonary Epithelial-Mesenchymal Transition in Systemic Sclerosis-Interstitial Lung Disease Model Mice by Intervening TGF-β1/Smad Signaling Pathway. Evidence-based Complementary and Alternative Medicine (PubMed: 35463079)

Application: WB Species: Mouse Sample: lung tissue

Figure 6 The expression levels of TGF-β1 (a), TβRI (b), TβRII (c), p-Smad2 (d), p-Smad3 (e), and Smad7 (f) protein and their protein band ((g), (h)) in the lung tissue of mice in each group. NC, normal control group; BLM, bleomycin-induced systemic sclerosis model group; PESV-L, low-dose PESV intervention group; PESV-M, medium-dose PESV intervention group; PESV-H, high-dose PESV intervention group; DXM, dexamethasone intervention group.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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GFRA1 Antibody - #DF7309