PEPD Antibody | Affinity Biosciences

1/2

PEPD Antibody - Western blot analysis of extracts from Rat lung, using PEPD Antibody.

PEPD Antibody - DF7333 at 1/100 staining Mouse lung tissue by IHC-P.

Product:	PEPD Antibody
Catalog:	DF7333
Description:	Rabbit polyclonal antibody to PEPD
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	54kDa; 55kD(Calculated).
Uniprot:	P12955
RRID:	AB_2839271

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(92%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(90%), Dog(100%), Chicken(92%), Xenopus(83%)

Clonality:

Polyclonal

Specificity:

PEPD Antibody detects endogenous levels of total PEPD.

RRID:

AB_2839271
Cite Format: Affinity Biosciences Cat# DF7333, RRID:AB_2839271.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Aminoacyl L proline hydrolase; Imidodipeptidase; MGC10905; MGC95081; Pep 4; Pep4; pepD; PEPD_HUMAN; Peptidase 4; Peptidase D; Prolidase; Proline dipeptidase; X pro dipeptidase; X-Pro dipeptidase; Xaa-Pro dipeptidase;

Immunogens

Immunogen:

Uniprot:

P12955(PEPD_HUMAN) >>Visit HPA database.

Gene(ID):

PEPD(5184)

Description:

This gene encodes a member of the peptidase family. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues. The enzyme serves an important role in the recycling of proline, and may be rate limiting for the production of collagen. Mutations in this gene result in prolidase deficiency, which is characterized by the excretion of large amount of di- and tri-peptides containing proline. Multiple transcript variants encoding different isoforms have been found for this gene.

Sequence:

P12955 PEPD_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAATGPSFWLGNETLKVPLALFALNRQRLCERLRKNPAVQAGSIVVLQGGEETQRYCTDTGVLFRQESFFHWAFGVTEPGCYGVIDVDTGKSTLFVPRLPASHATWMGKIHSKEHFKEKYAVDDVQYVDEIASVLTSQKPSVLLTLRGVNTDSGSVCREASFDGISKFEVNNTILHPEIVECRVFKTDMELEVLRYTNKISSEAHREVMKAVKVGMKEYELESLFEHYCYSRGGMRHSSYTCICGSGENSAVLHYGHAGAPNDRTIQNGDMCLFDMGGEYYCFASDITCSFPANGKFTADQKAVYEAVLRSSRAVMGAMKPGVWWPDMHRLADRIHLEELAHMGILSGSVDAMVQAHLGAVFMPHGLGHFLGIDVHDVGGYPEGVERIDEPGLRSLRTARHLQPGMVLTVEPGIYFIDHLLDEALADPARASFLNREVLQRFRGFGGVRIEEDVVVTDSGIELLTCVPRTVEEIEACMAGCDKAFTPFSGPK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Dog

100

Bovine

Sheep

Zebrafish

Chicken

Rabbit

Xenopus

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P12955 As Substrate

P12955

Site	PTM Type	Source
A2	Acetylation	Uniprot
K17	Acetylation	Uniprot
K36	Ubiquitination	Uniprot
K110	Acetylation	Uniprot
K110	Ubiquitination	Uniprot
S113	Phosphorylation	Uniprot
K114	Ubiquitination	Uniprot
K120	Ubiquitination	Uniprot
Y128	Phosphorylation	Uniprot
S167	Phosphorylation	Uniprot
T188	Phosphorylation	Uniprot
R196	Methylation	Uniprot
K200	Ubiquitination	Uniprot
K303	Ubiquitination	Uniprot
K484	Ubiquitination	Uniprot
T487	Phosphorylation	Uniprot
S490	Phosphorylation	Uniprot
K493	Acetylation	Uniprot

Research Backgrounds

P12955_PEPD_HUMAN

Function:

Splits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. Plays an important role in collagen metabolism because the high level of iminoacids in collagen.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the peptidase M24B family. Eukaryotic-type prolidase subfamily.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

PEPD Antibody - #DF7333